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MiR expression profiling at various stages of <jats:styled-content style=\"fixed-case\">NPC<\/jats:styled-content> was performed by miR array and further verified using quantitative real\u2010time <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. Pathway enrichment analysis was carried out to identify the functional pathways regulated by the miRs. The expression of a selected group of identified miRs was verified in stage I <jats:styled-content style=\"fixed-case\">NPC<\/jats:styled-content> by <jats:italic>in\u00a0situ<\/jats:italic> hybridization (<jats:styled-content style=\"fixed-case\">ISH<\/jats:styled-content>). A total of 449 miRs were identified with significantly different expressions between <jats:styled-content style=\"fixed-case\">NPC<\/jats:styled-content> tissues and normal pharyngeal tissues. Eighty\u2010four miRs were dysregulated only in stage I <jats:styled-content style=\"fixed-case\">NPC<\/jats:styled-content>, among which 45 miRs were up\u2010regulated and the other 39 were down\u2010regulated. Pathway enrichment assay revleaed that three significantly down\u2010regulated and three significantly up\u2010regulated miRs involved in 12 pathways associating with tumour formation and progression. Quantitative <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> confirmed the miR array result. In addition, the low expression levels of hsa\u2010miR\u20104324, hsa\u2010miR\u2010203a and hsa\u2010miR\u2010199b\u20105p were further validated in stage I <jats:styled-content style=\"fixed-case\">NPC<\/jats:styled-content> by <jats:styled-content style=\"fixed-case\">ISH<\/jats:styled-content>. This present study identifed the miR signature in stage I <jats:styled-content style=\"fixed-case\">NPC<\/jats:styled-content>, providing the basis for early detection and treatment of <jats:styled-content style=\"fixed-case\">NPC<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/jcmm.12906","type":"journal-article","created":{"date-parts":[[2016,8,4]],"date-time":"2016-08-04T10:53:48Z","timestamp":1470308028000},"page":"2259-2268","source":"Crossref","is-referenced-by-count":13,"title":["Distinctive micro<scp>RNA<\/scp> expression in early stage nasopharyngeal carcinoma patients"],"prefix":"10.1111","volume":"20","author":[{"given":"Shuna","family":"Li","sequence":"first","affiliation":[{"name":"Department of Otolaryngology and Head\u2010Neck Surgery Zhenjiang First People's Hospital The Affiliated People's Hospital of Jiangsu University Zhenjiang Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lihua","family":"Hang","sequence":"additional","affiliation":[{"name":"Department of Anesthesia Zhenjiang First People's Hospital The Affiliated People's Hospital of Jiangsu University Zhenjiang Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongming","family":"Ma","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology and Head\u2010Neck Surgery Zhenjiang First People's Hospital The Affiliated People's Hospital of Jiangsu University Zhenjiang Jiangsu China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chaoyang","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Radiation Oncology Zhenjiang First People's Hospital The Affiliated People's Hospital of Jiangsu University Zhenjiang Jiangsu 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