{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T08:25:44Z","timestamp":1790670344984,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2016,7,15]],"date-time":"2016-07-15T00:00:00Z","timestamp":1468540800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002803","name":"Fondazione Cariplo","doi-asserted-by":"publisher","award":["2013\u20100815"],"award-info":[{"award-number":["2013\u20100815"]}],"id":[{"id":"10.13039\/501100002803","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2016,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s (mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s) belong to a family of small non\u2010coding <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s (snc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s) playing important roles in human carcinogenesis. Multiple investigations reported mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s aberrantly expressed in several cancers, including high\u2010grade serous ovarian carcinoma (<jats:styled-content style=\"fixed-case\">HGS<\/jats:styled-content>\u2010OvCa). Quantitative PCR is widely used in studies investigating mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> expression and the identification of reliable endogenous controls is crucial for proper data normalization. In this study, we aimed to experimentally identify the most stable reference snc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s for normalization of mi<jats:styled-content style=\"fixed-case\">RNA qPCR<\/jats:styled-content> expression data in <jats:styled-content style=\"fixed-case\">HGS<\/jats:styled-content>\u2010OvCa. Eleven putative reference snc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s for normalization (U6, <jats:styled-content style=\"fixed-case\">SNORD<\/jats:styled-content>48, miR\u201092a\u20103p, let\u20107a\u20105p, <jats:styled-content style=\"fixed-case\">SNORD<\/jats:styled-content>61, <jats:styled-content style=\"fixed-case\">SNORD<\/jats:styled-content>72, <jats:styled-content style=\"fixed-case\">SNORD<\/jats:styled-content>68, miR\u2010103a\u20103p, miR\u2010423\u20103p, miR\u2010191\u20105p, miR\u201016\u20105p) were analysed on a total of 75 <jats:styled-content style=\"fixed-case\">HGS<\/jats:styled-content>\u2010OvCa and 30 normal tissues, using a highly specific <jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content>. Both the normal tissues considered to initiate <jats:styled-content style=\"fixed-case\">HGS<\/jats:styled-content>\u2010OvCa malignant transformation, namely ovary and fallopian tube epithelia, were included in our study. Stability of candidate endogenous controls was evaluated using an equivalence test and validated by geNorm and NormFinder algorithms. Combining results from the three different statistical approaches, <jats:styled-content style=\"fixed-case\">SNORD<\/jats:styled-content>48 emerged as stably and equivalently expressed between malignant and normal tissues. Among malignant samples, considering groups based on residual tumour, miR\u2010191\u20105p was identified as the most equivalent snc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>. On the basis of our results, we support the use of <jats:styled-content style=\"fixed-case\">SNORD<\/jats:styled-content>48 as best reference snc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> for relative quantification in mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> expression studies between <jats:styled-content style=\"fixed-case\">HGS<\/jats:styled-content>\u2010OvCa and normal controls, including the first time both the normal tissues supposed to be <jats:styled-content style=\"fixed-case\">HGS<\/jats:styled-content>\u2010OvCa progenitors. In addition, we recommend miR\u2010191\u20105p as best reference snc<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> in mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> expression studies with prognostic intent on <jats:styled-content style=\"fixed-case\">HGS<\/jats:styled-content>\u2010OvCa tissues.<\/jats:p>","DOI":"10.1111\/jcmm.12927","type":"journal-article","created":{"date-parts":[[2016,7,15]],"date-time":"2016-07-15T16:17:17Z","timestamp":1468599437000},"page":"2341-2348","source":"Crossref","is-referenced-by-count":55,"title":["Identification of stably expressed reference small non\u2010coding <scp>RNA<\/scp>s for micro<scp>RNA<\/scp> quantification in high\u2010grade serous ovarian carcinoma tissues"],"prefix":"10.1111","volume":"20","author":[{"given":"Eliana","family":"Bignotti","sequence":"first","affiliation":[{"name":"Division of Obstetrics and Gynecology ASST Spedali Civili di Brescia Brescia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefano","family":"Calza","sequence":"additional","affiliation":[{"name":"Department of Molecular and Translational Medicine University of Brescia Brescia Italy"},{"name":"Department of Medical Epidemiology and Biostatistics Karolinska Institutet Stockholm Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Renata A.","family":"Tassi","sequence":"additional","affiliation":[{"name":"\u201cAngelo Nocivelli\u201d Institute of Molecular Medicine Division of Obstetrics and Gynecology University of Brescia Brescia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laura","family":"Zanotti","sequence":"additional","affiliation":[{"name":"\u201cAngelo Nocivelli\u201d Institute of Molecular Medicine Division of Obstetrics and Gynecology University of Brescia Brescia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisabetta","family":"Bandiera","sequence":"additional","affiliation":[{"name":"\u201cAngelo Nocivelli\u201d Institute of Molecular Medicine Division of Obstetrics and Gynecology University of Brescia Brescia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Enrico","family":"Sartori","sequence":"additional","affiliation":[{"name":"Division of Obstetrics and Gynecology University of Brescia Brescia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franco E.","family":"Odicino","sequence":"additional","affiliation":[{"name":"Division of Obstetrics and Gynecology University of Brescia Brescia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonella","family":"Ravaggi","sequence":"additional","affiliation":[{"name":"\u201cAngelo Nocivelli\u201d Institute of Molecular Medicine Division of Obstetrics and Gynecology University of Brescia Brescia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paola","family":"Todeschini","sequence":"additional","affiliation":[{"name":"\u201cAngelo Nocivelli\u201d Institute of Molecular Medicine Division of Obstetrics and Gynecology University of Brescia Brescia Italy"},{"name":"Doctorate School of Molecular and Translational Medicine University of Milan Milan Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara","family":"Romani","sequence":"additional","affiliation":[{"name":"\u201cAngelo Nocivelli\u201d Institute of Molecular Medicine Division of Obstetrics and Gynecology University of Brescia Brescia Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,7,15]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0050232"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3389\/fonc.2014.00081"},{"issue":"1","key":"e_1_2_9_4_1","first-page":"S22","article-title":"Update on the management of ovarian cancer","volume":"8","author":"Ozols RF","year":"2002","journal-title":"Cancer J"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-07-1936"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0034546"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(04)00045-5"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.sbi.2005.05.006"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature10166"},{"key":"e_1_2_9_10_1","first-page":"517","article-title":"Cancer Genome Atlas Research Network. 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