{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T04:25:11Z","timestamp":1792297511248,"version":"4.4.0"},"reference-count":47,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2016,8,25]],"date-time":"2016-08-25T00:00:00Z","timestamp":1472083200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["8157041627"],"award-info":[{"award-number":["8157041627"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81400584"],"award-info":[{"award-number":["81400584"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2017,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Syndecan\u20101 (<jats:styled-content style=\"fixed-case\">SDC<\/jats:styled-content>1), with a variable ectodomain carrying heparan sulphate (<jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content>) chains between different Syndecans, participates in many steps of inflammatory responses. In the process of proteolysis, the <jats:styled-content style=\"fixed-case\">HS<\/jats:styled-content> chains of the complete extracellular domain can be shed from the cell surface, by which they can mediate most of <jats:styled-content style=\"fixed-case\">SDC<\/jats:styled-content>1's function. However, the exact impact on <jats:styled-content style=\"fixed-case\">SDC<\/jats:styled-content>1 which anchored on the cell surface has not been clearly reported. In our study, we established the models by transfection with the cleavable resistant <jats:styled-content style=\"fixed-case\">SDC<\/jats:styled-content>1 mutant plasmid, in which <jats:styled-content style=\"fixed-case\">SDC<\/jats:styled-content>1 shedding can be suppressed during stimulation. Role of membrane <jats:styled-content style=\"fixed-case\">SDC<\/jats:styled-content>1 in inflammatory pathway, pro\u2010inflammatory cytokine secretion as well as neutrophil transmigration, and how suppressing its shedding will benefit colitis were further investigated. We found that the patients suffered ulcerative colitis had high serum <jats:styled-content style=\"fixed-case\">SDC<\/jats:styled-content>1 levels,presented with increased levels of P65, tumour necrosis factor alpha (<jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b1) and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101\u03b2 and higher circulating neutrophils. <jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>\u2010\u03baB pathway was activated, and secretion of <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b1, interleukin\u20101beta (<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20101\u03b2), <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106 and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20108 were increased upon lipopolysaccharide stimuli in intestinal epithelial cells. Syndecan\u20101, <jats:italic>via<\/jats:italic> its anchored ectodomain, significantly lessened these up\u2010regulation extents. It also functioned in inhibiting transmigration of neutrophils by decreasing <jats:styled-content style=\"fixed-case\">CXCL<\/jats:styled-content>\u20101 secretion. Moreover, <jats:styled-content style=\"fixed-case\">SDC<\/jats:styled-content>1 ameliorated colitis activity and improved histological disturbances of colon in mice. Taken together, we conclude that suppression of <jats:styled-content style=\"fixed-case\">SDC<\/jats:styled-content>1 shedding from intestinal epithelial cells relieves severity of intestinal inflammation and neutrophil transmigration by inactivating key inflammatory regulators <jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>\u2010\u03baB, and down\u2010regulating pro\u2010inflammatory cytokine expressions. These indicated that compenstion and shedding suppression of cytomembrane <jats:styled-content style=\"fixed-case\">SDC<\/jats:styled-content>1 might be the optional therapy for intestinal inflammation.<\/jats:p>","DOI":"10.1111\/jcmm.12934","type":"journal-article","created":{"date-parts":[[2016,8,25]],"date-time":"2016-08-25T09:03:20Z","timestamp":1472115800000},"page":"13-25","source":"Crossref","is-referenced-by-count":38,"title":["Cell surface\u2010anchored syndecan\u20101 ameliorates intestinal inflammation and neutrophil transmigration in ulcerative colitis"],"prefix":"10.1111","volume":"21","author":[{"given":"Yan","family":"Zhang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Organ Failure Research Guangdong Provincial Key Laboratory of Gastroenterology Department of Gastroenterology Nanfang Hospital Southern Medical University Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongqiu","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Organ Failure Research Guangdong Provincial Key Laboratory of Gastroenterology Department of Gastroenterology Nanfang Hospital Southern Medical University Guangzhou Guangdong China"},{"name":"Department of Radiation Oncology and Cyberknife Center Key Laboratory of Cancer Prevention and Therapy Tianjin Medical University Cancer Institute &amp; Hospital National Clinical Research Center for Cancer Tianjin China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Liu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Organ Failure Research Guangdong Provincial Key Laboratory of Gastroenterology Department of Gastroenterology Nanfang Hospital Southern Medical University Guangzhou Guangdong China"},{"name":"Department of Gastroenterology Liuzhou Worker's Hospital Liuzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaoheng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Zhujiang Hospital Southern Medical University Guangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxi","family":"Fei","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Organ Failure Research Guangdong Provincial Key Laboratory of Gastroenterology Department of Gastroenterology Nanfang Hospital Southern Medical University Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jing","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Organ Failure Research Guangdong Provincial Key Laboratory of Gastroenterology Department of Gastroenterology Nanfang Hospital Southern Medical University Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ting","family":"Zhang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Organ Failure Research Guangdong Provincial Key Laboratory of Gastroenterology Department of Gastroenterology Nanfang Hospital Southern Medical University Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jide","family":"Wang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Organ Failure Research Guangdong Provincial Key Laboratory of Gastroenterology Department of Gastroenterology Nanfang Hospital Southern Medical University Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pyong W.","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Medicine Children's Hospital Harvard Medical School Boston MA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ye","family":"Chen","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Organ Failure Research Guangdong Provincial Key Laboratory of Gastroenterology Department of Gastroenterology Nanfang Hospital Southern Medical University Guangzhou Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,8,25]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)07499-8"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)60751-X"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1257"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1016-8478(23)07324-7"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.matbio.2011.10.001"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2012.178"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1097\/MIB.0000000000000421"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/febs.12174"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.330803"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M404506200"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(02)01079-6"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2010.080639"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.2741\/3542"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1001293"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2012.24"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2009-02-204966"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1155\/2005\/269076"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/ibd.20520"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/930124"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi050620i"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V83.3.799.799"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-010-1398-8"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2156"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.2174\/138945011796818261"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1097\/MIB.0b013e318280298f"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-015-3589-9"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00011-009-0091-x"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI32335"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.12523"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1097\/QAI.0b013e3181ecfeca"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M608542200"},{"key":"e_1_2_8_33_1","first-page":"456","article-title":"Cytokine\u2010induced neutrophil chemoattractant 1 (CINC\u20101) mediates the sympathetic component of inflammatory mechanical hypersensitivitiy in rats","volume":"13","author":"Lorenzetti BB","year":"2002","journal-title":"Eur Cytokine Netw"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M807001200"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M115.660852"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.42.4.477"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/nri2655"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1200057"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.12.040194.001041"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellimm.2014.09.005"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2014.07.016"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2014.286"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1003243"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0066397"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2036.1997.00252.x"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2036.2006.02870.x"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1527-3466.2004.tb00127.x"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-10-2476"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjcmm.12934","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjcmm.12934","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.12934","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,12]],"date-time":"2025-03-12T08:24:45Z","timestamp":1741767885000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.12934"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,8,25]]},"references-count":47,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2017,1]]}},"alternative-id":["10.1111\/jcmm.12934"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.12934","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,8,25]]}}}