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Twenty\u2010eight patients diagnosed with psoriasis vulgaris were selected as a case group with their lesional and non\u2010lesional skin tissues collected. A control group consisted of 20 patients who underwent plastic surgery with their healthy skin tissues collected. Real\u2010time quantitative fluorescence polymerase chain reaction (RT\u2010qPCR), <jats:italic>in situ<\/jats:italic> hybridization and immunohistochemistry were used to detect the expressions of miR\u2010181b and TLR4 in HEKs of healthy skin, psoriatic lesional skin and non\u2010lesional skin respectively. The 3\u2032 untranslated region (3\u2032UTR) of TLR4 combined with miR\u2010181b was verified by a dual\u2010luciferase reporter assay. Western blotting and bromodeoxyuridine were applied for corresponding detection of TLR4 expression and cell mitosis. The expression of miR\u2010181b in HEKs of psoriatic lesional skin was less than healthy skin and psoriatic non\u2010lesional skin. In psoriatic lesional and non\u2010lesional skin, TLR4\u2010positive cell rates and the number of positive cells per square millimetre were higher than healthy skin. The dual\u2010luciferase reporter assay verified that miR\u2010181b targets TLR4. HEKs transfected with miR\u2010181b mimics had decreased expression of TLR4, along with the decrease of mitotic indexes and Brdu labelling indexes. However, HEKs transfected with miR\u2010181b inhibitors showed increased TLR4 expression, mitotic indexes and Brdu labelling indexes. HEKs transfected with both miR\u2010181b inhibitors and siTLR4 had decreased mitotic indexes and Brdu labelling indexes. These results indicate that miR\u2010181b can negatively regulate the proliferation of HEKs in psoriasis by targeting TLR4.<\/jats:p>","DOI":"10.1111\/jcmm.12963","type":"journal-article","created":{"date-parts":[[2016,9,19]],"date-time":"2016-09-19T07:33:29Z","timestamp":1474270409000},"page":"278-285","source":"Crossref","is-referenced-by-count":30,"title":["<i>Retracted:<\/i> MicroRNA\u2010181b negatively regulates the proliferation of human epidermal keratinocytes in psoriasis through targeting TLR4"],"prefix":"10.1111","volume":"21","author":[{"given":"Cheng","family":"Feng","sequence":"first","affiliation":[{"name":"Aesthetic Plastic Department of Peking Union Medical Collage Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Bai","sequence":"additional","affiliation":[{"name":"Aesthetic Plastic Department of Peking Union Medical Collage Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan\u2010Ze","family":"Yu","sequence":"additional","affiliation":[{"name":"Aesthetic Plastic Department of Peking Union Medical Collage Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiao\u2010Jun","family":"Wang","sequence":"additional","affiliation":[{"name":"Aesthetic Plastic Department of Peking Union Medical Collage Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zeng","family":"Liu","sequence":"additional","affiliation":[{"name":"Aesthetic Plastic Department of Peking Union Medical Collage Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,9,19]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"67","article-title":"Psoriasis: epidemiology, natural history, and differential diagnosis","volume":"2","year":"2012","journal-title":"Psoriasis Targets Ther"},{"key":"e_1_2_7_3_1","first-page":"27","volume-title":"Psoriasis: epidemiology, Potential Triggers, Disease Course. 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