{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T00:08:42Z","timestamp":1790726922430,"version":"4.1.0"},"reference-count":37,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2016,9,19]],"date-time":"2016-09-19T00:00:00Z","timestamp":1474243200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation","doi-asserted-by":"crossref","award":["81471547"],"award-info":[{"award-number":["81471547"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation","doi-asserted-by":"crossref","award":["81273214"],"award-info":[{"award-number":["81273214"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation","doi-asserted-by":"crossref","award":["81172785"],"award-info":[{"award-number":["81172785"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Natural Science Fund of Jiangsu Province","award":["BK2011449"],"award-info":[{"award-number":["BK2011449"]}]},{"name":"Natural Science Fund of Jiangsu Province","award":["BK2008215"],"award-info":[{"award-number":["BK2008215"]}]},{"name":"Collaborative Fund of Yangzhou University and Yangzhou Municipality","award":["2012038-5"],"award-info":[{"award-number":["2012038-5"]}]},{"name":"Social Development Fund of Yangzhou Municipality","award":["YZ2014179"],"award-info":[{"award-number":["YZ2014179"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2017,2]]},"abstract":"Abstract<\/jats:title>Wogonin exerts anti\u2010tumour activities via<\/jats:italic> multiple mechanisms. We have identified that high\u2010dose wogonin (50 or 100 mg\/kg) could inhibit the growth of transplanted tumours by directly inducing tumour apoptosis and promoting DC<\/jats:styled-content>, T and NK<\/jats:styled-content> cell recruitment into tumour tissues to enhance immune surveillance. However, wogonin (20\u201350 \u03bcM) ex vivo<\/jats:italic> prevents inflammation by inhibiting NF<\/jats:styled-content>\u2010\u03baB and Erk signalling of macrophages and epithelial cells. It is elusive whether high\u2010dose wogonin promotes or prevents inflammation. To investigate the effects of high\u2010dose wogonin on murine colitis induced by dextran sodium sulphate (DSS<\/jats:styled-content>), mice were co\u2010treated with DSS<\/jats:styled-content> and various doses of wogonin. Intraperitoneal administration of wogonin (100 mg\/kg) exacerbated DSS<\/jats:styled-content>\u2010induced murine colitis. More CD<\/jats:styled-content>4+<\/jats:sup> CD<\/jats:styled-content>44+<\/jats:sup> and CD<\/jats:styled-content>8+<\/jats:sup> CD<\/jats:styled-content>44+<\/jats:sup> cells were located in the inflamed colons in the wogonin (100 mg\/kg) treatment group than in the other groups. Frequencies of CD<\/jats:styled-content>4+<\/jats:sup> CD<\/jats:styled-content>25+<\/jats:sup> CD<\/jats:styled-content>127\u2212<\/jats:sup> and CD<\/jats:styled-content>4+<\/jats:sup> CD<\/jats:styled-content>25+<\/jats:sup> Foxp3+<\/jats:sup> cells in the colons and spleen respectively, were reduced by wogonin treatment. Ex vivo<\/jats:italic> stimulations with high\u2010dose wogonin (50\u2013100 \u03bcg\/ml equivalent to 176\u2013352 \u03bcM) could synergize with IL<\/jats:styled-content>\u20102 to promote the functions of CD<\/jats:styled-content>4+<\/jats:sup> and CD<\/jats:styled-content>8+<\/jats:sup> cells. However, regulatory T cell induction was inhibited. Wogonin stimulated the activation of NF<\/jats:styled-content>\u2010\u03baB and Erk but down\u2010regulated STAT<\/jats:styled-content>3 phosphorylation in the CD<\/jats:styled-content>4+<\/jats:sup> T cells. Wogonin down\u2010regulated Erk and STAT<\/jats:styled-content>3\u2010Y705 phosphorylation in the regulatory T cells but promoted NF<\/jats:styled-content>\u2010\u03baB and STAT<\/jats:styled-content>3\u2010S727 activation. Our study demonstrated that high\u2010dose wogonin treatments would enhance immune activity by stimulating the effector T cells and by down\u2010regulating regulatory T cells.<\/jats:p>","DOI":"10.1111\/jcmm.12964","type":"journal-article","created":{"date-parts":[[2016,9,19]],"date-time":"2016-09-19T11:56:47Z","timestamp":1474286207000},"page":"286-298","source":"Crossref","is-referenced-by-count":19,"title":["High\u2010dose wogonin exacerbates DSS<\/scp>\u2010induced colitis by up\u2010regulating effector T cell function and inhibiting Treg cell"],"prefix":"10.1111","volume":"21","author":[{"given":"Weiming","family":"Xiao","sequence":"first","affiliation":[{"name":"Department of Gastroenterology Affiliated hospital Yangzhou University Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Min","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Affiliated hospital Yangzhou University Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keyan","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Affiliated hospital Yangzhou University Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guotao","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Affiliated hospital Yangzhou University Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bin","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Affiliated hospital Yangzhou University Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Li","family":"Qian","sequence":"additional","affiliation":[{"name":"Department of Immunology School of Medicine Yangzhou University Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoqing","family":"Jia","sequence":"additional","affiliation":[{"name":"Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yanbing","family":"Ding","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Affiliated hospital Yangzhou University Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Weijuan","family":"Gong","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Affiliated hospital Yangzhou University Yangzhou China"},{"name":"Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases Yangzhou China"},{"name":"Department of Immunology School of Medicine Yangzhou University Yangzhou China"},{"name":"Jiangsu Key Laboratory of Zoonosis Yangzhou China"},{"name":"Jiangsu Co\u2010innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses Yangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2016,9,19]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2014.05.011"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phymed.2012.10.016"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2010.07.015"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms13089877"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.3892\/or.2015.3896"},{"key":"e_1_2_7_7_1","first-page":"217","article-title":"Wogonin triggers apoptosis in human osteosarcoma U\u20102 OS cells through the endoplasmic reticulum stress, mitochondrial dysfunction and caspase\u20103\u2010dependent signaling pathways","volume":"39","author":"Lin CC","year":"2011","journal-title":"Int J Oncol"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2014.09.008"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.taap.2013.04.031"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.6796"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vph.2014.01.010"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2014.221"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/mc.21921"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10059-011-0041-7"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.intimp.2011.12.004"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2015.4353"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules20046888"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.22032"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/tox.21847"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0030294"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/imm.12305"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.3013"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/ptr.2645"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1271\/bbb.68.2505"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.3390\/molecules19022536"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00262-011-1130-3"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1097\/CJI.0000000000000080"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.315"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2007.258"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-10-313684"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00281-010-0212-1"},{"key":"e_1_2_7_32_1","first-page":"1409","article-title":"Harnessing regulatory T cells for the treatment of inflammatory bowel disease","volume":"21","author":"Geem D","year":"2015","journal-title":"Inflamm Bowel Dis"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-367-7_20"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2013.12.006"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1586\/17474124.2015.1065177"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.8815"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2008.448"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2013.11.005"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjcmm.12964","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjcmm.12964","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.12964","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jcmm.12964","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.12964","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,8,8]],"date-time":"2024-08-08T09:28:54Z","timestamp":1723109334000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.12964"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2016,9,19]]},"references-count":37,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2017,2]]}},"alternative-id":["10.1111\/jcmm.12964"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.12964","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2016,9,19]]}}}