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Pannexin 3 (Panx3), a member of Panxs (Pannexins), has been recently found to be involved in inflammation. However, the mechanism of Panx3 in human dental pulp inflammation remains unclear. In this study, the role of Panx3 in inflammatory response was firstly explored, and its potential mechanism was proposed. Immunohistochemical staining showed that Panx3 levels were diminished in inflamed human and rat dental pulp tissues. <jats:italic>In vitro<\/jats:italic>, Panx3 expression was significantly down\u2010regulated in <jats:styled-content style=\"fixed-case\">HDPC<\/jats:styled-content>s following a <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b1 challenge in a concentration\u2010dependent way, which reached the lowest level at 10 ng\/ml of <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b1. Such decrease could be reversed by <jats:styled-content style=\"fixed-case\">MG<\/jats:styled-content>132, a proteasome inhibitor. Unlike <jats:styled-content style=\"fixed-case\">MG<\/jats:styled-content>132, <jats:styled-content style=\"fixed-case\">BAY<\/jats:styled-content> 11\u20107082, a <jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>\u2010\u03baB inhibitor, even reinforced the inhibitory effect of <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b1. Quantitative real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">qRT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>) and enzyme\u2010linked immunosorbent assay (<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>) were used to investigate the role of Panx3 in inflammatory response of <jats:styled-content style=\"fixed-case\">HDPC<\/jats:styled-content>s. <jats:styled-content style=\"fixed-case\">TNF<\/jats:styled-content>\u2010\u03b1\u2010induced pro\u2010inflammatory cytokines, interleukin (<jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>)\u20101\u03b2 and <jats:styled-content style=\"fixed-case\">IL<\/jats:styled-content>\u20106, were significantly lessened when Panx3 was overexpressed in <jats:styled-content style=\"fixed-case\">HDPC<\/jats:styled-content>s. Conversely, Panx3 knockdown exacerbated the expression of pro\u2010inflammatory cytokines. Moreover, Western blot, dual\u2010luciferase reporter assay, immunofluorescence staining, <jats:styled-content style=\"fixed-case\">qRT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> results showed that Panx3 participated in dental pulp inflammation in a <jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>\u2010\u03baB\u2010dependent manner. These findings suggested that Panx3 has a defensive role in dental pulp inflammation, serving as a potential target to be exploited for the intervention of human dental pulp inflammation.<\/jats:p>","DOI":"10.1111\/jcmm.12988","type":"journal-article","created":{"date-parts":[[2016,9,29]],"date-time":"2016-09-29T07:48:26Z","timestamp":1475135306000},"page":"444-455","source":"Crossref","is-referenced-by-count":22,"title":["Pannexin3 inhibits <scp>TNF<\/scp>\u2010\u03b1\u2010induced inflammatory response by suppressing <scp>NF<\/scp>\u2010\u03baB signalling pathway in human dental pulp cells"],"prefix":"10.1111","volume":"21","author":[{"given":"Fangfang","family":"Song","sequence":"first","affiliation":[{"name":"The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei\u2010MOST) &amp; Key Laboratory of Oral Biomedicine Ministry of Education (KLOBM) School &amp; Hospital of Stomatology Wuhan University Wuhan Hubei 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