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The underlying molecular mechanisms of viral oncolysis are largely unknown. As cancer in companion animals shares striking similarities with human counterparts, we chose a permanent canine histiocytic sarcoma cell line (DH82 cells) to identify global transcriptome changes following infection with canine distemper virus (CDV), a paramyxovirus closely related to human measles virus. Microarray analysis identified 3054 differentially expressed probe sets (DEPs), encoding for 892 up\u2010 and 869 down\u2010regulated unique canine genes, respectively, in DH82 cells persistently infected with the vaccine strain Onderstepoort of CDV (DH82\u2010Ond\u2010pi), compared to non\u2010infected DH82 cells. Up\u2010regulated genes were predominantly related to immune processes, as demonstrated by functional enrichment analysis. Moreover, there was substantial enrichment of genes characteristic for classically activated M1 and alternatively activated M2 macrophages in DH82\u2010Ond\u2010pi; however, significant polarization into either of both categories was lacking. \u2018Angiogenesis\u2019 was the dominant enriched functional term for the down\u2010regulated genes, highlighting decreased blood vessel generation as a potential mechanism of paramyxovirus\u2010induced oncolysis in DH82 cells. The anti\u2010angiogenic effect of infection was verified by immunohistochemistry, which revealed a lower blood vessel density in an <jats:italic>in vivo<\/jats:italic> mouse model, xenotransplanted with DH82\u2010Ond\u2010pi, compared to mice transplanted with non\u2010infected DH82 cells. Reduction in angiogenesis appears to be an important oncolytic mechanism of CDV in DH82 cells, suggesting that similar mechanisms might account for human histiocytic sarcoma and maybe other tumours in conjunction with measles virus.<\/jats:p>","DOI":"10.1111\/jcmm.13023","type":"journal-article","created":{"date-parts":[[2016,11,17]],"date-time":"2016-11-17T06:55:17Z","timestamp":1479365717000},"page":"816-830","source":"Crossref","is-referenced-by-count":29,"title":["Reduced angiogenic gene expression in morbillivirus\u2010triggered oncolysis in a translational model for histiocytic sarcoma"],"prefix":"10.1111","volume":"21","author":[{"given":"Vanessa Maria","family":"Pfankuche","sequence":"first","affiliation":[{"name":"Department of Pathology University of Veterinary Medicine Hannover Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingo","family":"Spitzbarth","sequence":"additional","affiliation":[{"name":"Department of Pathology University of Veterinary 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