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The leading cause of OLF is thoracic spinal canal stenosis and myelopathy. In this study, the role of miR\u2010199b\u20105p and jagged 1 (JAG1) in primary ligamentum flavum cell osteogenesis was examined. MiR\u2010199b\u20105p was found to be down\u2010regulated during osteogenic differentiation in ligamentum flavum cells, while miR\u2010199b\u20105p overexpression inhibited osteogenic differentiation. In addition, JAG1 was found to be up\u2010regulated during osteogenic differentiation in ligamentum flavum cells, while JAG1 knockdown <jats:italic>via<\/jats:italic> RNA interference caused an inhibition of Notch signalling and osteogenic differentiation. Moreover, target prediction analysis and dual luciferase reporter assays supported the notion that JAG1 was a direct target of miR\u2010199b\u20105p, with miR\u2010199b\u20105p found to down\u2010regulate both JAG1 and Notch. Further, JAG1 knockdown was demonstrated to block the effect of miR\u2010199b\u20105p inhibition. These findings imply that miR\u2010199b\u20105p performs an inhibitory role in osteogenic differentiation in ligamentum flavum cells by potentially targeting JAG1 and influencing the Notch signalling pathway.<\/jats:p>","DOI":"10.1111\/jcmm.13047","type":"journal-article","created":{"date-parts":[[2016,12,13]],"date-time":"2016-12-13T07:32:45Z","timestamp":1481614365000},"page":"1159-1170","source":"Crossref","is-referenced-by-count":38,"title":["MiR\u2010199b\u20105p inhibits osteogenic differentiation in ligamentum flavum cells by targeting JAG1 and modulating the Notch signalling pathway"],"prefix":"10.1111","volume":"21","author":[{"given":"Xiaochen","family":"Qu","sequence":"first","affiliation":[{"name":"Department of Orthopaedics Peking University Third Hospital Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhongqiang","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Orthopaedics Peking University Third Hospital 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