{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T18:53:46Z","timestamp":1790708026273,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2016,12,20]],"date-time":"2016-12-20T00:00:00Z","timestamp":1482192000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004497","name":"Onderzoeksraad, KU Leuven","doi-asserted-by":"publisher","award":["EF\/95\/010"],"award-info":[{"award-number":["EF\/95\/010"]}],"id":[{"id":"10.13039\/501100004497","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2017,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The mast\/stem cell growth factor receptor <jats:styled-content style=\"fixed-case\">KIT<\/jats:styled-content> has long been assumed to be a specific marker for interstitial cells of Cajal (<jats:styled-content style=\"fixed-case\">ICC<\/jats:styled-content>) in the bladder, with possible druggable perspectives. However, several authors have challenged the presence of <jats:styled-content style=\"fixed-case\">KIT<\/jats:styled-content><jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">ICC<\/jats:styled-content> in recent years. The aim of this study was therefore to attempt to clarify the conflicting reports on <jats:styled-content style=\"fixed-case\">KIT<\/jats:styled-content> expression in the bladder of human beings, rat, mouse and guinea pig and to elucidate the possible role of antibody\u2010related issues and interspecies differences in this matter. Fresh samples were obtained from human, rat, mouse and guinea pig cystectomies and processed for single\/double immunohistochemistry\/immunofluorescence. Specific antibodies against <jats:styled-content style=\"fixed-case\">KIT<\/jats:styled-content>, mast cell tryptase (<jats:styled-content style=\"fixed-case\">MCT<\/jats:styled-content>), anoctamin\u20101 (<jats:styled-content style=\"fixed-case\">ANO<\/jats:styled-content>1) and vimentin were used to characterize the cell types expressing <jats:styled-content style=\"fixed-case\">KIT<\/jats:styled-content>. Gut (jejunum) tissue was used as an external antibody control. Our results revealed <jats:styled-content style=\"fixed-case\">KIT<\/jats:styled-content> expression on mast cells but not on <jats:styled-content style=\"fixed-case\">ICC<\/jats:styled-content> in human, rat, mouse and guinea pig bladder. Parallel immunohistochemistry showed <jats:styled-content style=\"fixed-case\">KIT<\/jats:styled-content> expression on <jats:styled-content style=\"fixed-case\">ICC<\/jats:styled-content> in human, rat, mouse and guinea pig gut, which confirmed the selectivity of the <jats:styled-content style=\"fixed-case\">KIT<\/jats:styled-content> antibody clones. In conclusion, we have shown that <jats:styled-content style=\"fixed-case\">KIT<\/jats:styled-content><jats:sup>+<\/jats:sup> cells in human, rat, mouse and guinea pig bladder are mast cells and not <jats:styled-content style=\"fixed-case\">ICC<\/jats:styled-content>. The present report is important as it opposes the idea that <jats:styled-content style=\"fixed-case\">KIT<\/jats:styled-content><jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">ICC<\/jats:styled-content> are present in bladder. In this perspective, functional concepts of <jats:styled-content style=\"fixed-case\">KIT<\/jats:styled-content><jats:sup>+<\/jats:sup> <jats:styled-content style=\"fixed-case\">ICC<\/jats:styled-content> being involved in sensory and\/or motor aspects of bladder physiology should be revised.<\/jats:p>","DOI":"10.1111\/jcmm.13054","type":"journal-article","created":{"date-parts":[[2016,12,20]],"date-time":"2016-12-20T21:48:54Z","timestamp":1482270534000},"page":"1206-1216","source":"Crossref","is-referenced-by-count":23,"title":["The stem cell growth factor receptor <scp>KIT<\/scp> is not expressed on interstitial cells in bladder"],"prefix":"10.1111","volume":"21","author":[{"given":"Thomas","family":"Gevaert","sequence":"first","affiliation":[{"name":"Laboratory of Experimental Urology Organ Systems KU Leuven Leuven Belgium"},{"name":"Translational Cell and Tissue Research Department of Imaging and Pathology KU Leuven Leuven Belgium"},{"name":"Department of Pathology AZ Klina 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