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A group of mice underwent endurance training while another set led a sedentary lifestyle and were freely mobile within cages. All experimental groups were treated with either <jats:styled-content style=\"fixed-case\">ND<\/jats:styled-content> or peanut oil at different doses for 6 weeks. Testosterone serum levels were measured <jats:italic>via<\/jats:italic> liquid chromatography\u2013mass spectrometry. Western blot analysis and quantitative real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> were utilized to determine gene and protein expression levels of the primary enzymes implicated in testosterone biosynthesis and gene expression levels of the blood\u2013testis barrier (<jats:styled-content style=\"fixed-case\">BTB<\/jats:styled-content>) components. Immunohistochemistry and immunofluorescence were conducted for testicular morphological evaluation. The study demonstrated that moderate to high doses of <jats:styled-content style=\"fixed-case\">ND<\/jats:styled-content> induced a diminished serum testosterone level and altered the expression level of the key steroidogenic enzymes involved in testosterone biosynthesis. At the morphological level, <jats:styled-content style=\"fixed-case\">ND<\/jats:styled-content> induced degradation of the <jats:styled-content style=\"fixed-case\">BTB<\/jats:styled-content> by targeting the tight junction protein\u20101 (<jats:styled-content style=\"fixed-case\">TJP<\/jats:styled-content>1). <jats:styled-content style=\"fixed-case\">ND<\/jats:styled-content> stimulation deregulated metalloproteinase\u20109, metalloproteinase\u20102 (MMP\u20102) and the tissue inhibitor of MMP\u20102. Moreover, <jats:styled-content style=\"fixed-case\">ND<\/jats:styled-content> administration resulted in a mislocalization of mucin\u20101. In conclusion, <jats:styled-content style=\"fixed-case\">ND<\/jats:styled-content> abuse induces a decline in testosterone production that is unable to regulate the internalization and redistribution of <jats:styled-content style=\"fixed-case\">TJP<\/jats:styled-content>1 and may induce the deregulation of other <jats:styled-content style=\"fixed-case\">BTB<\/jats:styled-content> constituents <jats:italic>via<\/jats:italic> the inhibition of <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>\u20102. <jats:styled-content style=\"fixed-case\">ND<\/jats:styled-content> may well be considered as both a potential inducer of male infertility and a potential risk factor to a low endogenous bioavailable testosterone.<\/jats:p>","DOI":"10.1111\/jcmm.13092","type":"journal-article","created":{"date-parts":[[2017,2,28]],"date-time":"2017-02-28T12:04:14Z","timestamp":1488283454000},"page":"1636-1647","source":"Crossref","is-referenced-by-count":31,"title":["Nandrolone decanoate 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