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However, the role of EZH2 in liver fibrosis is largely unknown. In this study, it was identified that EZH2 promoted Wnt pathway\u2010stimulated fibroblasts <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic> by repressing Dkk\u20101, which is a Wnt pathway antagonist. The expression of EZH2 was increased in CCl<jats:sub>4<\/jats:sub>\u2010induced rat liver and primary HSCs as well as TGF\u2010\u03b21\u2010treated HSC\u2010T6, whereas the expression of Dkk1 was reduced. Silencing of EZH2 prevented TGF\u2010\u03b21\u2010induced proliferation of HSC\u2010T6 cells and the expression of \u03b1\u2010SMA. In addition, knockdown of Dkk1 promoted TGF\u2010\u03b21\u2010induced activation of HSCs. Moreover, silencing of EZH2 could restore the repression of Dkk\u20101 through trimethylation of H3K27me3 in TGF\u2010\u03b21\u2010treated HSC\u2010T6 cells. Interestingly, inhibition of EZH2 had almost no effect on the activation of HSC when Dkk1 was silenced. Collectively, EZH2\u2010mediated repression of Dkk1 promotes the activation of Wnt\/\u03b2\u2010catenin pathway, which is an essential event for HSC activation.<\/jats:p>","DOI":"10.1111\/jcmm.13153","type":"journal-article","created":{"date-parts":[[2017,3,24]],"date-time":"2017-03-24T03:30:35Z","timestamp":1490326235000},"page":"2317-2328","source":"Crossref","is-referenced-by-count":57,"title":["EZH2\u2010mediated repression of Dkk1 promotes hepatic stellate cell activation and hepatic fibrosis"],"prefix":"10.1111","volume":"21","author":[{"given":"Yang","family":"Yang","sequence":"first","affiliation":[{"name":"The key of Laboratory Precision Medicine for Severe Autoimmune Diseases of Anhui Province Anhui Institute of Innovative Drugs Anhui Medical University Hefei China"},{"name":"The Key Laboratory of Anti\u2010inflammatory and Immune Medicines Ministry of Education Hefei China"},{"name":"Institute for Liver Diseases of Anhui Medical University Hefei 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