{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T01:46:01Z","timestamp":1793151961421,"version":"4.5.0"},"reference-count":43,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2017,5,24]],"date-time":"2017-05-24T00:00:00Z","timestamp":1495584000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Natural Science Fund","award":["81000730"],"award-info":[{"award-number":["81000730"]}]},{"name":"National Natural Science Fund","award":["81470851"],"award-info":[{"award-number":["81470851"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2017,11]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>\n                    Mesenchymal stem cell (MSC) transplantation alone may be insufficient for treatment of liver fibrosis because of complicated histopathological changes in the liver. Given that miR\u2010122 plays an essential role in liver fibrosis by negatively regulating the proliferation and transactivation of hepatic stellate cells (HSCs), this study investigated whether miR\u2010122 modification can improve the therapeutic efficacy of adipose tissue\u2010derived MSCs in treating liver fibrosis. MiR\u2010122\u2010modified AMSCs (AMSC\u2010122) were constructed through lentivirus\u2010mediated transfer of pre\u2010miR\u2010122. MiR\u2010122\u2010modified AMSCs expressed high level of miR\u2010122, while they retained their phenotype and differentiation potential as na\u00efve AMSCs. AMSC\u2010122 more effectively suppressed the proliferation of and collagen maturation in HSCs than scramble miRNA\u2010modified AMSCs. In addition, AMSC\u2010derived exosomes mediated the miR\u2010122 communication between AMSCs and HSCs, further affecting the expression levels of miR\u2010122 target genes, such as insulin\u2010like growth factor receptor 1 (IGF1R), Cyclin G(1) (CCNG1) and prolyl\u20104\u2010hydroxylase \u03b11 (P4HA1), which are involved in proliferation of and collagen maturation in HSCs. Moreover, miR\u2010122 modification enhanced the therapeutic efficacy of AMSCs in the treatment of carbon tetrachloride (CCl\n                    <jats:sub>4<\/jats:sub>\n                    )\u2010induced liver fibrosis by suppressing the activation of HSCs and alleviating collagen deposition. Results demonstrate that miR\u2010122 modification improves the therapeutic efficacy of AMSCs through exosome\u2010mediated miR\u2010122 communication; thus, miR\u2010122 modification is a new potential strategy for treatment of liver fibrosis.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.13208","type":"journal-article","created":{"date-parts":[[2017,5,25]],"date-time":"2017-05-25T13:06:35Z","timestamp":1495717595000},"page":"2963-2973","source":"Crossref","is-referenced-by-count":196,"title":["MiR\u2010122 modification enhances the therapeutic efficacy of adipose tissue\u2010derived mesenchymal stem cells against liver fibrosis"],"prefix":"10.1111","volume":"21","author":[{"given":"Guohua","family":"Lou","sequence":"first","affiliation":[{"name":"Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases State Key Laboratory for Diagnosis and Treatment of Infectious Diseases The First Affiliated Hospital School of Medicine Zhejiang University Hangzhou 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