{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T06:43:07Z","timestamp":1790318587288,"version":"4.1.0"},"reference-count":0,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,6,13]],"date-time":"2017-06-13T00:00:00Z","timestamp":1497312000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000925","name":"National Health and Medical Research Council","doi-asserted-by":"publisher","award":["1032695"],"award-info":[{"award-number":["1032695"]}],"id":[{"id":"10.13039\/501100000925","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2017,12]]},"abstract":"Abstract<\/jats:title>The extracellular matrix (ECM<\/jats:styled-content>) creates the microenvironment of the tissue; an altered ECM<\/jats:styled-content> in the asthmatic airway may be central in airway inflammation and remodelling. Tumstatin is a collagen IV<\/jats:styled-content>\u2010derived matrikine reduced in the asthmatic airway wall that reverses airway inflammation and remodelling in small and large animal models of asthma. This study hypothesized that the mechanisms underlying the broad asthma\u2010resolving effects of tumstatin were due to autocrine remodelling of the ECM<\/jats:styled-content>. Neutrophils and endothelial cells were seeded on decellularized ECM<\/jats:styled-content> of non\u2010asthmatic (NA<\/jats:styled-content>) or asthmatic (A) airway smooth muscle (ASM<\/jats:styled-content>) cells previously exposed to tumstatin in the presence or absence of a broad matrix metalloproteinase inhibitor, Marimastat. Gene expression in NA<\/jats:styled-content> and A ASM<\/jats:styled-content> induced by tumstatin was assessed using RT<\/jats:styled-content>\u2010PCR<\/jats:styled-content> arrays. The presence of tumstatin during ECM<\/jats:styled-content> deposition affected neutrophil and endothelial cell properties on both NA<\/jats:styled-content> and A ASM<\/jats:styled-content>\u2010derived matrices and this was only partly due to MMP<\/jats:styled-content> activity. Gene expression patterns in response to tumstatin in NA<\/jats:styled-content> and A ASM<\/jats:styled-content> cells were different. Tumstatin may foster an anti\u2010inflammatory and anti\u2010angiogenic microenvironment by modifying ASM<\/jats:styled-content>\u2010derived ECM<\/jats:styled-content>. Further work is required to examine whether restoring tumstatin levels in the asthmatic airway represents a potential novel therapeutic approach.<\/jats:p>","DOI":"10.1111\/jcmm.13232","type":"journal-article","created":{"date-parts":[[2017,6,13]],"date-time":"2017-06-13T13:44:25Z","timestamp":1497361465000},"page":"3288-3297","source":"Crossref","is-referenced-by-count":36,"title":["Tumstatin regulates the angiogenic and inflammatory potential of airway smooth muscle extracellular matrix"],"prefix":"10.1111","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-1665-3482","authenticated-orcid":false,"given":"Louise Margaret","family":"Harkness","sequence":"first","affiliation":[{"name":"Respiratory Cell and Molecular Biology Woolcock Institute of Medical Research Sydney NSW Australia"},{"name":"Discipline of Pharmacology The University of Sydney Sydney NSW Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Markus","family":"Weckmann","sequence":"additional","affiliation":[{"name":"Section for Pediatric Pneumology and Allergology University Medical Center Schleswig\u2010Holstein Campus Centrum Luebeck Airway Research Centre North (ARCN) Member of the German Centre of Lung Research (DZL) Luebeck Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthias","family":"Kopp","sequence":"additional","affiliation":[{"name":"Section for Pediatric Pneumology and Allergology University Medical Center Schleswig\u2010Holstein Campus Centrum Luebeck Airway Research Centre North (ARCN) Member of the German Centre of Lung Research (DZL) Luebeck Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tim","family":"Becker","sequence":"additional","affiliation":[{"name":"Fraunhofer Institute for Marine Biotechnology (Fraunhofer EMB) Luebeck Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony Wayne","family":"Ashton","sequence":"additional","affiliation":[{"name":"Division of Perinatal Research Kolling Institute of Medical Research Sydney NSW Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janette Kay","family":"Burgess","sequence":"additional","affiliation":[{"name":"Respiratory Cell and Molecular Biology Woolcock Institute of Medical Research Sydney NSW Australia"},{"name":"Discipline of Pharmacology The University of Sydney Sydney NSW Australia"},{"name":"University of Groningen University Medical Center Groningen Department of Pathology and Medical Biology Groningen Research Institute for Asthma and COPD (GRIAC) Groningen The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,6,13]]},"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjcmm.13232","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.13232","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,1]],"date-time":"2023-10-01T15:33:05Z","timestamp":1696174385000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.13232"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,6,13]]},"references-count":0,"journal-issue":{"issue":"12","published-print":{"date-parts":[[2017,12]]}},"alternative-id":["10.1111\/jcmm.13232"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.13232","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,6,13]]}}}