{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,29]],"date-time":"2026-07-29T17:30:45Z","timestamp":1785346245655,"version":"3.55.0"},"reference-count":45,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,6,13]],"date-time":"2017-06-13T00:00:00Z","timestamp":1497312000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["nsfc 81260286"],"award-info":[{"award-number":["nsfc 81260286"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study was aimed to explore the role of miR\u201029b\u20103p and <jats:styled-content style=\"fixed-case\">PGRN<\/jats:styled-content> in chondrocyte apoptosis and the initiation and progress of osteoarthritis (<jats:styled-content style=\"fixed-case\">OA<\/jats:styled-content>). Both miR\u201029b\u20103p and <jats:styled-content style=\"fixed-case\">PGRN<\/jats:styled-content> were up\u2010regulated in cartilage tissue from patients with <jats:styled-content style=\"fixed-case\">OA<\/jats:styled-content>. Transfection of miR\u201029b\u20103p mimic into rat primary chondrocytes and <jats:styled-content style=\"fixed-case\">SW<\/jats:styled-content>1353 chondrosarcoma cells significantly suppressed <jats:styled-content style=\"fixed-case\">PGRN<\/jats:styled-content> expression and release, induced apoptosis, inhibited proliferation and scratch wound closure. By contrast, transfection of miR\u201029b\u20103p inhibitor exhibited the opposite effects. Moreover, the expression and secretion of cartilaginous degeneration\u2010related molecules were also altered by miR\u201029b\u20103p. Luciferase reporter gene assay showed rat <jats:styled-content style=\"fixed-case\">GRN mRNA<\/jats:styled-content> is directly targeted and repressed by miR\u201029b\u20103p. The fact that recombinant <jats:styled-content style=\"fixed-case\">PGRN<\/jats:styled-content> or sh<jats:styled-content style=\"fixed-case\">PGRN<\/jats:styled-content>\u2010mediated <jats:styled-content style=\"fixed-case\">PGRN<\/jats:styled-content> interference abolished miR\u201029b\u20103p mimic\u2010induced cell apoptosis and growth inhibition suggested miR\u201029b\u20103p affect the cellular functions of chondrocyte through regulating <jats:styled-content style=\"fixed-case\">PGRN<\/jats:styled-content> expression. <jats:italic>In vivo<\/jats:italic>, joint cavity injection of miR\u201029b\u20103p antagomir prior to surgical induction of <jats:styled-content style=\"fixed-case\">OA<\/jats:styled-content> significantly suppressed the upregulation of miR\u201029b\u20103p, whereas further promoted the increased expression of <jats:styled-content style=\"fixed-case\">PGRN<\/jats:styled-content>. Articular chondrocytes apoptosis and cartilage loss in the knee joint of surgically induced <jats:styled-content style=\"fixed-case\">OA<\/jats:styled-content> rats were also ameliorated by the injection of miR\u201029b\u20103p antagomir, demonstrated by <jats:styled-content style=\"fixed-case\">TUNEL<\/jats:styled-content> and safranin O\u2010fast green staining. This work showed miR\u201029b\u20103p facilitates chondrocyte apoptosis and OA by targeting <jats:styled-content style=\"fixed-case\">PGRN<\/jats:styled-content>, and miR\u201029b\u20103p or <jats:styled-content style=\"fixed-case\">PGRN<\/jats:styled-content> may be the potential target for <jats:styled-content style=\"fixed-case\">OA<\/jats:styled-content> treatments.<\/jats:p>","DOI":"10.1111\/jcmm.13237","type":"journal-article","created":{"date-parts":[[2017,6,13]],"date-time":"2017-06-13T13:12:47Z","timestamp":1497359567000},"page":"3347-3359","source":"Crossref","is-referenced-by-count":110,"title":["MiR\u201029b\u20103p promotes chondrocyte apoptosis and facilitates the occurrence and development of osteoarthritis by targeting <scp>PGRN<\/scp>"],"prefix":"10.1111","volume":"21","author":[{"given":"Lingqiang","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Orthopaedics The First Affiliated Hospital of Kunming Medical University Kunming Yunnan Province 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