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Although recent findings demonstrate that catalase, autophagy and the nuclear factor \u03baB (NF\u2010\u03baB) signalling pathway are centrally involved in diabetic cardiomyopathy (DCM), the interplay between the three has not been fully characterized. Thus, the mechanism responsible for catalase\u2010mediated protection against heart injury in diabetic mice was investigated in this study, as well as the role of NF\u2010\u03baB\u2010p65 in the regulation of autophagic flux was investigated in this study. Western blot analysis revealed that catalase inhibited NF\u2010\u03baB activity and decreased LC3\u2010II (microtubule\u2010associated protein 1 light chain 3) and beclin\u20101 (Atg6) expression. Furthermore, up\u2010regulation of autophagy was detrimental for cardiac function in diabetic mice. Catalase overexpression reduced the level of NF\u2010\u03baB subunit in the nucleus, where it initiates autophagy through activation of the key autophagy gene <jats:italic>BECN1<\/jats:italic>. To evaluate the role of the NF\u2010\u03baB pathway in diabetes\u2010induced autophagy, Bay11\u20107082, an NF\u2010\u03baB inhibitor, was injected into diabetic mice, which suppressed NF\u2010\u03baB and attenuated diabetes\u2010induced autophagy and myocardial apoptosis. In agreement with the <jats:italic>in vivo<\/jats:italic> results, Bay11\u20107082 also inhibited high\u2010glucose\u2010induced activation of NF\u2010\u03baB and the up\u2010regulation of LC3\u2010II and beclin\u20101 expression in H9c2 cells. In addition, high\u2010glucose\u2010induced activation of autophagic flux and apoptosis were largely attenuated by p65 siRNA, suggesting that catalase ameliorates diabetes\u2010induced autophagy, at least in part by increasing the activity of the NF\u2010\u03baB pathway and p65\u2010mediated transcription of BECN1.<\/jats:p>","DOI":"10.1111\/jcmm.13252","type":"journal-article","created":{"date-parts":[[2017,6,23]],"date-time":"2017-06-23T07:23:20Z","timestamp":1498202600000},"page":"3420-3434","source":"Crossref","is-referenced-by-count":18,"title":["Catalase ameliorates diabetes\u2010induced cardiac injury through reduced p65\/RelA\u2010 mediated transcription of <i>BECN1<\/i>"],"prefix":"10.1111","volume":"21","author":[{"given":"Xu","family":"Wang","sequence":"first","affiliation":[{"name":"School of Pharmaceutical Science Wenzhou Medical University Wenzhou 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