{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T09:38:04Z","timestamp":1790329084011,"version":"4.1.0"},"reference-count":53,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,7,17]],"date-time":"2017-07-17T00:00:00Z","timestamp":1500249600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Telocytes (<jats:styled-content style=\"fixed-case\">TC<\/jats:styled-content>) are typically defined as cells with telopodes by their ultrastructural features. Their presence was reported in the interstitium of various organs in vertebrates, including humans. However, no study has yet described the presence of <jats:styled-content style=\"fixed-case\">TC<\/jats:styled-content> in the human eye and in particular, within the stromal compartment of the cornea. To address this issue, samples of normal and pathologic (keratoconic) human corneas were tested by immunohistochemistry for <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>34, platelet\u2010derived growth factor receptor \u03b1 (<jats:styled-content style=\"fixed-case\">PDGFR<\/jats:styled-content>\u03b1) and c\u2010kit\/<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>117 or examined by transmission electron microscopy. We found that <jats:styled-content style=\"fixed-case\">TC<\/jats:styled-content> coexpressing <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>34 and <jats:styled-content style=\"fixed-case\">PDGFR<\/jats:styled-content>\u03b1 were distributed throughout the whole normal corneal stroma with different <jats:styled-content style=\"fixed-case\">TC<\/jats:styled-content> subtypes being distinguishable on the basis of the expression of the stemness marker c\u2010kit (<jats:italic>i.e<\/jats:italic>. c\u2010kit\u2010positive and c\u2010kit\u2010negative <jats:styled-content style=\"fixed-case\">TC<\/jats:styled-content> subpopulations). Transmission electron microscopy examination confirmed the existence of spindle\u2010shaped and bipolar <jats:styled-content style=\"fixed-case\">TC<\/jats:styled-content> typically displaying two long and thin moniliform telopodes establishing intercellular contacts formed by gap junctions. Keratoconic corneas were characterized by ultrastructural damages and patchy loss of <jats:styled-content style=\"fixed-case\">TC<\/jats:styled-content> with an almost complete depletion of the c\u2010kit\u2010positive <jats:styled-content style=\"fixed-case\">TC<\/jats:styled-content> subpopulation. We propose that <jats:styled-content style=\"fixed-case\">TC<\/jats:styled-content> may contribute to the maintenance of corneal stromal homoeostasis and that, in particular, the c\u2010kit\u2010positive <jats:styled-content style=\"fixed-case\">TC<\/jats:styled-content> subtype might have stemness capacity participating in corneal regeneration and repair processes. Further studies are needed to clarify the differential roles of corneal <jats:styled-content style=\"fixed-case\">TC<\/jats:styled-content> subtypes as well as the possible therapeutic applications of <jats:styled-content style=\"fixed-case\">TC<\/jats:styled-content> in degenerative corneal disorders such as keratoconus.<\/jats:p>","DOI":"10.1111\/jcmm.13270","type":"journal-article","created":{"date-parts":[[2017,7,17]],"date-time":"2017-07-17T11:02:41Z","timestamp":1500289361000},"page":"3602-3611","source":"Crossref","is-referenced-by-count":45,"title":["Telocytes in normal and keratoconic human cornea: an immunohistochemical and transmission electron microscopy study"],"prefix":"10.1111","volume":"21","author":[{"given":"Mirca","family":"Marini","sequence":"first","affiliation":[{"name":"Department of Experimental and Clinical Medicine Section of Anatomy and Histology University of Florence Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rita","family":"Mencucci","sequence":"additional","affiliation":[{"name":"Eye Clinic Department of Surgery and Translational Medicine University of Florence Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene","family":"Rosa","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Medicine Section of Anatomy and Histology University of Florence Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleonora","family":"Favuzza","sequence":"additional","affiliation":[{"name":"Eye Clinic Department of Surgery and Translational Medicine University of Florence Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniele","family":"Guasti","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Medicine Section of Anatomy and Histology University of Florence Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lidia","family":"Ibba\u2010Manneschi","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Medicine Section of Anatomy and Histology University of Florence Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3956-8480","authenticated-orcid":false,"given":"Mirko","family":"Manetti","sequence":"additional","affiliation":[{"name":"Department of Experimental and Clinical Medicine Section of Anatomy and Histology University of Florence Florence Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,7,17]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2014.09.003"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exer.2010.06.021"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.brainresbull.2009.05.019"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1097\/00003226-200003000-00012"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.3341\/kjo.2005.19.3.213"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-9071.2010.02207.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajo.2013.06.025"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00417-014-2836-7"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1442-9071.2004.00805.x"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-10-1061-3_1"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1515\/BMC.2011.039"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1515\/bmc-2014-0029"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.semcdb.2016.05.019"},{"key":"e_1_2_7_15_1","first-page":"7","article-title":"Telocyte \u2010 a particular cell phenotype. 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