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Here, we investigated the contribution of specific components of the gastric refluxate on adhesion molecules involved in epithelial barrier maintenance. Cell line models of squamous epithelium (<jats:styled-content style=\"fixed-case\">HET<\/jats:styled-content>\u20101A) and <jats:styled-content style=\"fixed-case\">BO<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">QH<\/jats:styled-content>) were used to examine the effects of bile acids on cell adhesion to extracellular matrix proteins (Collagen, laminin, vitronectin, fibronectin) and expression of integrin ligands (\u03b1<jats:sub>3<\/jats:sub>, \u03b1<jats:sub>4,<\/jats:sub> \u03b1<jats:sub>5<\/jats:sub>, \u03b1<jats:sub>6<\/jats:sub> and \u03b1<jats:sub>\u03bd<\/jats:sub>). Experimental findings were validated in human explant oesophageal biopsies, a rat model of gastroesophageal reflux disease (<jats:styled-content style=\"fixed-case\">GORD<\/jats:styled-content>) and in patient tissue microarrays. The bile acid deoxycholic acid (<jats:styled-content style=\"fixed-case\">DCA<\/jats:styled-content>) specifically reduced adhesion of <jats:styled-content style=\"fixed-case\">HET<\/jats:styled-content>\u20101A cells to vitronectin and reduced cell\u2010surface expression of integrin\u2010\u03b1<jats:sub>\u03bd<\/jats:sub> <jats:italic>via<\/jats:italic> effects on endocytic recycling processes. Increased expression of integrin\u2010\u03b1<jats:sub>v<\/jats:sub> was observed in ulcerated tissue in a rat model of <jats:styled-content style=\"fixed-case\">GORD<\/jats:styled-content> and in oesophagitis and Barrett's intestinal metaplasia patient tissue compared to normal squamous epithelium. Increased expression of integrin\u2010\u03b1<jats:sub>\u03bd<\/jats:sub> was observed in <jats:styled-content style=\"fixed-case\">QH BO<\/jats:styled-content> cells compared to <jats:styled-content style=\"fixed-case\">HET<\/jats:styled-content>\u20101A cells. <jats:styled-content style=\"fixed-case\">QH<\/jats:styled-content> cells were resistant to <jats:styled-content style=\"fixed-case\">DCA<\/jats:styled-content>\u2010mediated loss of adhesion and reduction in cell\u2010surface expression of integrin\u2010\u03b1<jats:sub>\u03bd<\/jats:sub>. We demonstrated that a specific component of the gastric refluxate, <jats:styled-content style=\"fixed-case\">DCA<\/jats:styled-content>, affects the epithelial barrier through modulation of integrin \u03b1<jats:sub>\u03bd<\/jats:sub> expression, providing a novel mechanism for bile acid\u2010mediated erosion of oesophageal squamous epithelium and promotion of <jats:styled-content style=\"fixed-case\">BO<\/jats:styled-content>. Strategies aimed at preventing bile acid\u2010mediated erosion should be considered in the clinical management of patients with <jats:styled-content style=\"fixed-case\">GORD<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/jcmm.13271","type":"journal-article","created":{"date-parts":[[2017,9,23]],"date-time":"2017-09-23T04:10:38Z","timestamp":1506139838000},"page":"3612-3625","source":"Crossref","is-referenced-by-count":21,"title":["Deoxycholic acid promotes development of gastroesophageal reflux disease and Barrett's oesophagus by modulating integrin\u2010\u03b1v trafficking"],"prefix":"10.1111","volume":"21","author":[{"given":"David O.","family":"Prichard","sequence":"first","affiliation":[{"name":"Cell and Molecular Biology Group Department of Clinical Medicine Trinity Translational Medicine Institute Trinity College Dublin St James's Hospital Dublin 8 Ireland"},{"name":"Division of Gastroenterology Mayo Clinic Health System La Crosse \u2013 Franciscan Healthcare La Crosse WI USA"},{"name":"Division of Gastroenterology Mayo Clinic Rochester MN USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne Marie","family":"Byrne","sequence":"additional","affiliation":[{"name":"Cell and Molecular Biology Group Department of Clinical Medicine Trinity Translational Medicine Institute Trinity College Dublin St James's Hospital Dublin 8 Ireland"},{"name":"Department of Surgery Trinity Translational Medicine Institute Trinity College Dublin St James's Hospital Dublin 8 Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"James O.","family":"Murphy","sequence":"additional","affiliation":[{"name":"Department of Surgery Trinity Translational Medicine Institute Trinity College Dublin St James's Hospital Dublin 8 Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"John V.","family":"Reynolds","sequence":"additional","affiliation":[{"name":"Department of Surgery Trinity Translational Medicine Institute Trinity College Dublin St James's Hospital Dublin 8 Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jacintha","family":"O'Sullivan","sequence":"additional","affiliation":[{"name":"Department of Surgery Trinity Translational Medicine Institute Trinity College Dublin St James's Hospital Dublin 8 Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronan","family":"Feighery","sequence":"additional","affiliation":[{"name":"Department of Surgery Trinity Translational Medicine Institute Trinity College Dublin St James's Hospital Dublin 8 Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Brendan","family":"Doyle","sequence":"additional","affiliation":[{"name":"Department of Histopathology St James's Hospital Dublin 8 Ireland"},{"name":"Department of Histopathology Beaumont Hospital Dublin 9 Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Osama Sharaf","family":"Eldin","sequence":"additional","affiliation":[{"name":"Department of Histopathology St James's Hospital Dublin 8 Ireland"},{"name":"Faculty of Medicine Mansoura University Mansoura Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stephen P.","family":"Finn","sequence":"additional","affiliation":[{"name":"Department of Histopathology St James's Hospital Dublin 8 Ireland"},{"name":"Department of Histopathology and Morbid Anatomy Trinity College Dublin Dublin Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aoife","family":"Maguire","sequence":"additional","affiliation":[{"name":"Department of Histopathology St James's Hospital Dublin 8 Ireland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Deirdre","family":"Duff","sequence":"additional","affiliation":[{"name":"Cell and Molecular Biology Group Department of Clinical Medicine Trinity Translational Medicine Institute Trinity College Dublin St James's Hospital 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