{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,19]],"date-time":"2026-02-19T04:42:48Z","timestamp":1771476168976,"version":"3.50.1"},"reference-count":35,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2017,8,1]],"date-time":"2017-08-01T00:00:00Z","timestamp":1501545600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002949","name":"Government of Jiangsu Province","doi-asserted-by":"publisher","award":["RC201152"],"award-info":[{"award-number":["RC201152"]}],"id":[{"id":"10.13039\/501100002949","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81570565"],"award-info":[{"award-number":["81570565"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We explored the role of micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u201030a (miR\u201030a) and the mechanism involved in hepatic fibrosis. MiR\u201030a overexpression was achieved by miR\u201030a mimics transfection in hepatic stellate cells (<jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content>s) (<jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content>\u2010T6, <jats:styled-content style=\"fixed-case\">LX<\/jats:styled-content>\u20102), and miR\u201030a agomir (ago\u2010miR\u201030a) treatment in mice. MiR\u201030a levels were measured using TaqMan mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> assay system, and the localization of miR\u201030a was detected by fluorescence <jats:italic>in\u00a0situ<\/jats:italic> hybridization (<jats:styled-content style=\"fixed-case\">FISH<\/jats:styled-content>). The interaction of miR\u201030a and Beclin1 was confirmed by dual\u2010luciferase reporter assay. Autophagic flux was analysed using tandem <jats:styled-content style=\"fixed-case\">mRFP<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">GFP<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>3 fluorescence microscopy, electron microscopy and Western blot of <jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>3\u2010<jats:styled-content style=\"fixed-case\">II<\/jats:styled-content>\/I ratio. MiR\u201030a was notably down\u2010regulated in activated <jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content>s and <jats:styled-content style=\"fixed-case\">LX<\/jats:styled-content>\u20102\u2010exosomes induced by <jats:styled-content style=\"fixed-case\">TGF<\/jats:styled-content>\u2010\u03b21; overexpression of miR\u201030a down\u2010regulated extracellular matrix (<jats:styled-content style=\"fixed-case\">ECM<\/jats:styled-content>), such as \u03b1\u2010<jats:styled-content style=\"fixed-case\">SMA<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> TIMP<\/jats:styled-content>\u20101, and Collagen I expression, and suppressed cell viability in <jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content>s. MiR\u201030a was significantly down\u2010regulated in hepatic fibrosis mice and overexpression of miR\u201030a prevented <jats:styled-content style=\"fixed-case\">BDL<\/jats:styled-content>\u2010induced fibrogenesis, concomitant with the down\u2010regulation of <jats:styled-content style=\"fixed-case\">ECM<\/jats:styled-content>. MiR\u201030a inhibited <jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content>s autophagy and increased lipid accumulation in <jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content>s and in mice fibrotic hepatic tissues. MiR\u201030a inhibited its downstream effector of Beclin1 by direct targeting its 3\u2032\u2010<jats:styled-content style=\"fixed-case\">UTR<\/jats:styled-content> region. Moreover, Knock\u2010down of Beclin1 by small interfering <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> (si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>) inhibited <jats:styled-content style=\"fixed-case\">HSC<\/jats:styled-content> autophagy and activation in <jats:styled-content style=\"fixed-case\">LX<\/jats:styled-content>\u20102 cells. In conclusion, miR\u201030a is down\u2010regulated in hepatic fibrosis models and its overexpression prevents liver fibrogenesis by directly suppressing Beclin1\u2010mediated autophagy; therefore, miR\u201030a may be a new potential therapeutic target for controlling hepatic fibrosis.<\/jats:p>","DOI":"10.1111\/jcmm.13278","type":"journal-article","created":{"date-parts":[[2017,8,2]],"date-time":"2017-08-02T09:29:52Z","timestamp":1501666192000},"page":"3679-3692","source":"Crossref","is-referenced-by-count":105,"title":["Micro<scp>RNA<\/scp>\u201030a ameliorates hepatic fibrosis by inhibiting Beclin1\u2010mediated autophagy"],"prefix":"10.1111","volume":"21","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9853-5526","authenticated-orcid":false,"given":"Jianliang","family":"Chen","sequence":"first","affiliation":[{"name":"Department of Liver Surgery The First Affiliated Hospital of Nanjing Medical University Nanjing Jiangsu Province China"},{"name":"Key Laboratory of Living Donor Liver Transplantation of Ministry of Public Health Nanjing Jiangsu Province China"},{"name":"Department of General Surgery People's Hospital Jingjiang Jiangsu Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Liver Surgery The First Affiliated Hospital of Nanjing Medical University Nanjing Jiangsu Province China"},{"name":"Key Laboratory of Living Donor Liver Transplantation of Ministry of Public Health Nanjing Jiangsu Province China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shu","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Liver Surgery The First Affiliated Hospital of Nanjing Medical University Nanjing Jiangsu Province China"},{"name":"Key Laboratory of Living Donor Liver Transplantation of Ministry of Public Health Nanjing Jiangsu Province 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