{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,16]],"date-time":"2025-10-16T20:32:04Z","timestamp":1760646724664,"version":"3.37.3"},"reference-count":36,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2017,10,27]],"date-time":"2017-10-27T00:00:00Z","timestamp":1509062400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","award":["NRF\u20102014R1A2A1A10050406"],"award-info":[{"award-number":["NRF\u20102014R1A2A1A10050406"]}],"id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The G12 family of G protein alpha subunits has been shown to participate in the regulation of various physiological processes. However, the role of G\u03b112 in bone physiology has not been well described. Here, by micro\u2010<jats:styled-content style=\"fixed-case\">CT<\/jats:styled-content> analysis, we discovered that G\u03b112\u2010knockout mice have an osteopetrotic phenotype. Histological examination showed lower osteoclast number in femoral tissue of G\u03b112\u2010knockout mice compared to wild\u2010type mice. Additionally, <jats:italic>in vitro<\/jats:italic> osteoclastic differentiation of precursor cells with receptor activator of nuclear factor\u2010\u03baB ligand (<jats:styled-content style=\"fixed-case\">RANKL<\/jats:styled-content>) showed that G\u03b112 deficiency decreased the number of osteoclast generated and the bone resorption activity. The induction of nuclear factor of activated T\u2010cell c1 (<jats:styled-content style=\"fixed-case\">NFAT<\/jats:styled-content>c1), the key transcription factor of osteoclastogenesis, and the activation of RhoA by <jats:styled-content style=\"fixed-case\">RANKL<\/jats:styled-content> was also significantly suppressed by G\u03b112 deficiency. We further found that the <jats:styled-content style=\"fixed-case\">RANKL<\/jats:styled-content> induction of <jats:styled-content style=\"fixed-case\">NFAT<\/jats:styled-content>c1 was not dependent on RhoA signalling, while osteoclast precursor migration and bone resorption required RhoA in the G\u03b112\u2010mediated regulation of osteoclasts. Therefore, G\u03b112 plays a role in differentiation through <jats:styled-content style=\"fixed-case\">NFAT<\/jats:styled-content>c1 and in cell migration and resorption activity through RhoA during osteoclastogenesis.<\/jats:p>","DOI":"10.1111\/jcmm.13370","type":"journal-article","created":{"date-parts":[[2017,10,27]],"date-time":"2017-10-27T13:52:39Z","timestamp":1509112359000},"page":"849-860","source":"Crossref","is-referenced-by-count":16,"title":["G\u03b112 regulates osteoclastogenesis by modulating <scp>NFAT<\/scp>c1 expression"],"prefix":"10.1111","volume":"22","author":[{"given":"Min\u2010Kyoung","family":"Song","sequence":"first","affiliation":[{"name":"Department of Cell and Developmental Biology BK21 Program and Dental Research Institute Seoul National University Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cheolkyu","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology BK21 Program and Dental Research Institute Seoul National University Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yong Deok","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology BK21 Program and Dental Research Institute Seoul National University Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haemin","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology BK21 Program and Dental Research Institute Seoul National University Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min Kyung","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology BK21 Program and Dental Research Institute Seoul National University Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun\u2010Oh","family":"Kwon","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology BK21 Program and Dental Research Institute Seoul National University Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5738-1105","authenticated-orcid":false,"given":"Ja Hyun","family":"Koo","sequence":"additional","affiliation":[{"name":"College of Pharmacy and Research Institute of Pharmaceutical Sciences Seoul National University Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min Sung","family":"Joo","sequence":"additional","affiliation":[{"name":"College of Pharmacy and Research Institute of Pharmaceutical Sciences Seoul National University Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sang Geon","family":"Kim","sequence":"additional","affiliation":[{"name":"College of Pharmacy and Research Institute of Pharmaceutical Sciences Seoul National University Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1411-6106","authenticated-orcid":false,"given":"Hong\u2010Hee","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Cell and Developmental Biology BK21 Program and Dental Research Institute Seoul National University Seoul Korea"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,10,27]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0301-472X(99)00061-2"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.12.7724"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00109-004-0612-6"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.11005\/jbm.2014.21.4.233"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1902986"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.88.13.5582"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1994.1129"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.14.6741"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2008.08.002"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm1666"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.5.2985"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.280.5372.2109"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0234057100"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.9.5868"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi700235f"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.021350998"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1038\/27454"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.102291599"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1204189"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.1860"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1208665"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.42.24631"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M508690200"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.275.16.11993"},{"issue":"6","key":"e_1_2_7_26_1","doi-asserted-by":"crossref","first-page":"2285","DOI":"10.1242\/jcs.108.6.2285","article-title":"The small GTP\u2010binding protein, rho p21, is involved in bone resorption by regulating cytoskeletal organization in osteoclasts","volume":"108","author":"Zhang D","year":"1995","journal-title":"J Cell Sci"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1236"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1101254"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M113.461848"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms4773"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2016.11.005"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tips.2005.01.007"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvn118"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1210\/en.2004-1283"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0898-6568(03)00131-1"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.22883"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1517\/17460441.2.12.1591"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjcmm.13370","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.13370","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T12:12:23Z","timestamp":1695903143000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.13370"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,10,27]]},"references-count":36,"journal-issue":{"issue":"2","published-print":{"date-parts":[[2018,2]]}},"alternative-id":["10.1111\/jcmm.13370"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.13370","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"type":"print","value":"1582-1838"},{"type":"electronic","value":"1582-4934"}],"subject":[],"published":{"date-parts":[[2017,10,27]]}}}