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In parallel, the effects of such a loss were evaluated on vascular endothelial growth factor (VEGF) and transforming growth factor \u03b21 (TGF\u03b21) gene and protein expression in fibroblasts and macrophages in cell culture. Histological, immunohistochemical and quantitative RT\u2010PCR changes determined if <jats:italic>Tnx<\/jats:italic> gene ablation on angiogenic gene expression, inflammatory cell infiltration and neovascularization induced by central corneal stromal cauterization. The role was determined of <jats:italic>Tnx<\/jats:italic> function in controlling VEGF\u2010A or TGF\u03b21 gene expression by comparing their expression levels in ocular fibroblasts and macrophages obtained from wild\u2010type (WT) and body\u2010wide <jats:italic>Tnx<\/jats:italic> KO mice. Tnx was up\u2010regulated in cauterized cornea. In Tnx KO, macrophage invasion was attenuated, VEGF\u2010A and its cognate receptor mRNA expression along with neovascularization were lessened in <jats:italic>Tnx<\/jats:italic> KOs relative to the changes occurring in their WT counterpart. Loss of <jats:italic>Tnx<\/jats:italic> instead up\u2010regulated <jats:italic>in\u00a0vivo<\/jats:italic> mRNA expression of anti\u2010angiogenic VEGF\u2010B but not VEGF\u2010A. On the other hand, TGF\u03b21 mRNA expression declined in <jats:italic>Tnx<\/jats:italic> KO cultured ocular fibroblasts. Loss of <jats:italic>Tnx<\/jats:italic> gene expression caused VEGF\u2010A expression to decline in macrophages. <jats:italic>Tnx<\/jats:italic> gene expression contributes to promoting TGF\u03b21 mRNA expression in ocular fibroblasts and VEGF\u2010A in macrophages, macrophage invasion, up\u2010regulation of VEGF\u2010A expression and neovascularization in an injured corneal stroma. On the other hand, it suppresses anti\u2010angiogenic VEGF\u2010B mRNA expression <jats:italic>in\u00a0vivo<\/jats:italic>.<\/jats:p>","DOI":"10.1111\/jcmm.13397","type":"journal-article","created":{"date-parts":[[2017,11,21]],"date-time":"2017-11-21T05:12:45Z","timestamp":1511241165000},"page":"948-956","source":"Crossref","is-referenced-by-count":13,"title":["Loss of tenascin X gene function impairs injury\u2010induced stromal angiogenesis in mouse corneas"],"prefix":"10.1111","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8061-0041","authenticated-orcid":false,"given":"Takayoshi","family":"Sumioka","sequence":"first","affiliation":[{"name":"Department of Ophthalmology Wakayama Medical University School of Medicine Wakayama Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroki","family":"Iwanishi","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology Wakayama Medical University School of Medicine Wakayama 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