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The S1P1 receptor is associated with nitric oxide (<jats:styled-content style=\"fixed-case\">NO<\/jats:styled-content>)\u2010mediated <jats:styled-content style=\"fixed-case\">SM<\/jats:styled-content> relaxation, while S1P2 &amp; S1P3 receptors are linked to <jats:styled-content style=\"fixed-case\">SM<\/jats:styled-content> contraction <jats:italic>via<\/jats:italic> activation of the Rho\u2010kinase pathway. This study is to determine testosterone (T) modulating the expression and functional activity of S1P receptors in corpus cavernosum (<jats:styled-content style=\"fixed-case\">CC<\/jats:styled-content>). Adult male Sprague\u2010Dawley rats were randomly divided into three groups: sham\u2010operated controls, surgical castration and T supplemented group. Serum S1P levels were detected by high\u2010performance liquid chromatography. The expression of S1P1\u20103 receptors and sphingosine kinases was detected by real\u2010time <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. <jats:italic>In vitro<\/jats:italic> organ bath contractility and <jats:italic>in\u00a0vivo<\/jats:italic> intracavernous pressure (<jats:styled-content style=\"fixed-case\">ICP<\/jats:styled-content>) measurement were also performed. T deprivation significantly decreased <jats:styled-content style=\"fixed-case\">ICP<\/jats:styled-content> rise. Meanwhile, surgical castration induced a significant increase in serum S1P level and the expression of S1P2\u20103 receptors by twofold (<jats:italic>P\u00a0<\/jats:italic>&lt;<jats:italic>\u00a0<\/jats:italic>0.05) but a decrease in the expression of S1P1 receptor. Castration also augmented exogenous phenylephrine (<jats:styled-content style=\"fixed-case\">PE<\/jats:styled-content>), S1P, S1P1,3 receptor agonist <jats:styled-content style=\"fixed-case\">FTY<\/jats:styled-content>720\u2010P contractility and S1P2\u2010specific antagonist <jats:styled-content style=\"fixed-case\">JTE<\/jats:styled-content>013 relaxation effect. T supplemented could restore the aforementioned changes. We provide novel data that castration increased serum S1P concentration and up\u2010regulated the expression of S1P2\u20103 receptors in <jats:styled-content style=\"fixed-case\">CC<\/jats:styled-content>. Consistently, agonizing S1P receptors induced <jats:styled-content style=\"fixed-case\">CCSM<\/jats:styled-content> contraction and antagonizing mediated relaxation were augmented. This provides the first clear evidence that S1P system dysregulation may contribute to hypogonadism\u2010related erectile dysfunction (<jats:styled-content style=\"fixed-case\">ED<\/jats:styled-content>), and S1P receptors may be expected as a potential target for treating <jats:styled-content style=\"fixed-case\">ED<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/jcmm.13416","type":"journal-article","created":{"date-parts":[[2017,12,20]],"date-time":"2017-12-20T06:39:40Z","timestamp":1513751980000},"page":"1507-1516","source":"Crossref","is-referenced-by-count":14,"title":["Testosterone regulates the expression and functional activity of sphingosine\u20101\u2010phosphate receptors in the rat corpus cavernosum"],"prefix":"10.1111","volume":"22","author":[{"given":"Jing","family":"Yin","sequence":"first","affiliation":[{"name":"Department of Rehabilitation Zhongnan Hospital of Wuhan University Wuhan 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