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As <jats:styled-content style=\"fixed-case\">TPN<\/jats:styled-content>\u2010associated complications are partially related to choline deficiency, we aimed to address whether choline supplementation could attenuate <jats:styled-content style=\"fixed-case\">OOLE<\/jats:styled-content>\u2010induced enterocyte apoptosis. Herein we present evidence that supplementary choline exhibits protective effect against <jats:styled-content style=\"fixed-case\">OOLE<\/jats:styled-content>\u2010induced enterocyte apoptosis both <jats:italic>in vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic>. In a rat model of <jats:styled-content style=\"fixed-case\">TPN<\/jats:styled-content>, substantial reduction in apoptotic rate along with decreased expression of <jats:styled-content style=\"fixed-case\">CELF<\/jats:styled-content>1 was observed when supplementary choline was added to <jats:styled-content style=\"fixed-case\">OOLE<\/jats:styled-content>. In cultured Caco\u20102 cells, supplementary choline attenuated <jats:styled-content style=\"fixed-case\">OOLE<\/jats:styled-content>\u2010induced apoptosis and mitochondria dysfunction by suppressing <jats:styled-content style=\"fixed-case\">CELF<\/jats:styled-content>1\/<jats:styled-content style=\"fixed-case\">AIF<\/jats:styled-content> pathway. Compared to <jats:styled-content style=\"fixed-case\">OOLE<\/jats:styled-content> alone, the expression of <jats:styled-content style=\"fixed-case\">CELF<\/jats:styled-content>1 and <jats:styled-content style=\"fixed-case\">AIF<\/jats:styled-content> was significantly decreased by supplementary choline, whereas the expression of Bcl\u20102 was evidently increased. No obvious alterations were observed in Bax expression and caspase\u20103 activation. Mechanistically, supplementary choline repressed the expression of <jats:styled-content style=\"fixed-case\">CELF<\/jats:styled-content>1 by increasing the recruitment of <jats:styled-content style=\"fixed-case\">CELF<\/jats:styled-content>1 <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> to processing bodies, thus resulting in suppression of its protein translation. Taken together, our data suggest that supplementary choline exhibits effective protection against <jats:styled-content style=\"fixed-case\">OOLE<\/jats:styled-content>\u2010induced enterocyte apoptosis, and thus, it has the potential to be used for the prevention and treatment of <jats:styled-content style=\"fixed-case\">TPN<\/jats:styled-content>\u2010induced intestinal atrophy.<\/jats:p>","DOI":"10.1111\/jcmm.13430","type":"journal-article","created":{"date-parts":[[2017,11,6]],"date-time":"2017-11-06T11:46:43Z","timestamp":1509968803000},"page":"1562-1573","source":"Crossref","is-referenced-by-count":13,"title":["Supplementary choline attenuates olive oil lipid emulsion\u2010induced enterocyte apoptosis through suppression of <scp>CELF<\/scp>1\/<scp>AIF<\/scp> pathway"],"prefix":"10.1111","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6593-9279","authenticated-orcid":false,"given":"Jun\u2010Kai","family":"Yan","sequence":"first","affiliation":[{"name":"Xin Hua 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