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Paired bladder cancer and adjacent tissue samples (102) and normal bladder tissue samples (106) were obtained. Bladder cancer cell lines (T24, 5637, RT<\/jats:styled-content>4, 253J and J82) were selected and assigned to blank, negative control (NC<\/jats:styled-content>), TGF<\/jats:styled-content>\u2010\u03b2, thrombospondin\u20101 (TSP<\/jats:styled-content>\u20101), TGF<\/jats:styled-content>\u2010\u03b21+ TSP<\/jats:styled-content>\u20101, GP<\/jats:styled-content>73\u2010siRNA<\/jats:styled-content>\u20101, GP<\/jats:styled-content>73\u2010siRNA<\/jats:styled-content>\u20102, GP<\/jats:styled-content>73\u2010siRNA<\/jats:styled-content>\u20101+ TSP<\/jats:styled-content>\u20101, GP<\/jats:styled-content>73\u2010siRNA<\/jats:styled-content>\u20101+ pcDNA<\/jats:styled-content>\u2010GP<\/jats:styled-content>73, WT<\/jats:styled-content>1\u2010siRNA<\/jats:styled-content> and WT<\/jats:styled-content>1\u2010siRNA<\/jats:styled-content> + GP<\/jats:styled-content>73\u2010siRNA<\/jats:styled-content>\u20101 groups. Expressions of GP<\/jats:styled-content>73, TGF<\/jats:styled-content>\u2010\u03b21, Smad2, p\u2010Smad2, E\u2010cadherin and vimentin were detected using RT<\/jats:styled-content>\u2010qPCR<\/jats:styled-content> and Western blotting. Cell proliferation, migration and invasion were determined using MTT<\/jats:styled-content> assay, scratch testing and Transwell assay, respectively. Compared with the blank and NC<\/jats:styled-content> groups, levels of GP<\/jats:styled-content>73, TGF<\/jats:styled-content>\u2010\u03b21, Smad2, p\u2010Smad2, N\u2010cadherin and vimentin decreased, and levels of WT<\/jats:styled-content>1 and E\u2010cadherin increased in the GP<\/jats:styled-content>73\u2010siRNA<\/jats:styled-content>\u20101 and GP<\/jats:styled-content>73\u2010siRNA<\/jats:styled-content>\u20102 groups, while the opposite results were observed in the WT<\/jats:styled-content>1 siRNA<\/jats:styled-content>, TGF<\/jats:styled-content>\u2010\u03b2, TSP<\/jats:styled-content>\u20101 and TGF<\/jats:styled-content>\u2010\u03b2 + TSP<\/jats:styled-content>\u20101 groups. Cell proliferation, migration and invasion notably decreased in the GP<\/jats:styled-content>73\u2010siRNA<\/jats:styled-content>\u20101 and GP<\/jats:styled-content>73\u2010siRNA<\/jats:styled-content>\u20102 groups in comparison with the blank and NC<\/jats:styled-content> groups, while in the WT<\/jats:styled-content>1 siRNA<\/jats:styled-content>, TGF<\/jats:styled-content>\u2010\u03b2, TSP<\/jats:styled-content>\u20101 and TGF<\/jats:styled-content>\u2010\u03b2 + TSP<\/jats:styled-content>\u20101 groups, cell migration, invasion and proliferation showed the reduction after the EMT<\/jats:styled-content>. These results suggest that GP<\/jats:styled-content>73 promotes bladder cancer invasion and metastasis by inducing the EMT<\/jats:styled-content> through down\u2010regulating WT<\/jats:styled-content>1 levels and activating the TGF<\/jats:styled-content>\u2010\u03b21\/Smad2 signalling pathway.<\/jats:p>","DOI":"10.1111\/jcmm.13442","type":"journal-article","created":{"date-parts":[[2018,1,19]],"date-time":"2018-01-19T05:09:18Z","timestamp":1516338558000},"page":"1650-1665","source":"Crossref","is-referenced-by-count":25,"title":["GP<\/scp>73 promotes invasion and metastasis of bladder cancer by regulating the epithelial\u2013mesenchymal transition through the TGF<\/scp>\u2010\u03b21\/Smad2 signalling pathway"],"prefix":"10.1111","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8164-2253","authenticated-orcid":false,"given":"Han\u2010Jie","family":"Yang","sequence":"first","affiliation":[{"name":"Department of Urology Pingxiang Affiliated, Southern Medical University 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