{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,27]],"date-time":"2026-07-27T00:01:28Z","timestamp":1785110488257,"version":"3.55.0"},"reference-count":37,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,1,24]],"date-time":"2018-01-24T00:00:00Z","timestamp":1516752000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81200789"],"award-info":[{"award-number":["81200789"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The objective of this study was to investigate the advantages and feasibility of periodontal tissue regeneration using platelet\u2010rich fibrin (PRF) combined with rat periodontal ligament stem cells (PDLSCs) for the first time. We first determined the effect of PRF on rat PDLSCs <jats:italic>in\u00a0vitro<\/jats:italic>. We next conducted an <jats:italic>in\u00a0vivo<\/jats:italic> study, in which a tissue engineering technique was performed to repair periodontal defects in five groups: a blank group, collagen group (implanted collagen membrane), collagen\u00a0+\u00a0cells group (implanted collagen membrane and rat PDLSCs), PRF group (implanted PRF membrane) and PRF\u00a0+\u00a0cells group (implanted PRF membrane and rat PDLSCs). PRF greatly enhanced cell proliferation, mRNA and protein expression levels of bone sialoprotein (BSP), osteocalcin (OC), and runt\u2010related transcription factor 2 (RUNX2) and activity of alkaline phosphatase (ALP) <jats:italic>in\u00a0vitro<\/jats:italic>. Transplantation of PRF combined with rat PDLSCs resulted in higher expression of osteopontin (Opn), collagen I (COL1A) and RUNX2 at both 12 and 24\u00a0days after surgery. Micro\u2010computed tomography and histological analysis showed substantially more new bone formation in the PRF\u00a0+\u00a0cells group at 24\u00a0days after surgery. Based on these results, we discuss the role of PRF in the proliferation and differentiation of rat PDLSCs and suggest that PRF combined with rat PDLSCs provides a valuable tool for periodontal tissue engineering.<\/jats:p>","DOI":"10.1111\/jcmm.13461","type":"journal-article","created":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T12:08:39Z","timestamp":1695902919000},"page":"1047-1055","source":"Crossref","is-referenced-by-count":41,"title":["Study of platelet\u2010rich fibrin combined with rat periodontal ligament stem cells in periodontal tissue regeneration"],"prefix":"10.1111","volume":"22","author":[{"given":"Xuejing","family":"Duan","sequence":"first","affiliation":[{"name":"School of Stomatology Shandong Provincial Hospital affiliated to Shandong University Jinan Shandong Province China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhiyong","family":"Lin","sequence":"additional","affiliation":[{"name":"School of Stomatology Shandong Provincial Hospital 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