{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T06:30:53Z","timestamp":1787725853742,"version":"build-2784847793"},"reference-count":38,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2018,1,12]],"date-time":"2018-01-12T00:00:00Z","timestamp":1515715200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81273533"],"award-info":[{"award-number":["81273533"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81473238"],"award-info":[{"award-number":["81473238"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["ZR2014HM087"],"award-info":[{"award-number":["ZR2014HM087"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2018,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Therapeutic agents are urgently needed for treating metastatic castration\u2010refractory prostate cancer (<jats:styled-content style=\"fixed-case\">mCRPC<\/jats:styled-content>) that is unresponsive to androgen deprivation and chemotherapy. Our screening assays demonstrated that chemotherapy\u2010resistant prostate cancer (<jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content>a) cells are more sensitive to <jats:styled-content style=\"fixed-case\">HDAC<\/jats:styled-content> inhibitors than paired sensitive <jats:styled-content style=\"fixed-case\">PC<\/jats:styled-content>a cells, as demonstrated by cell proliferation and apoptosis <jats:italic>in\u00a0vitro<\/jats:italic> and <jats:italic>in\u00a0vivo<\/jats:italic>. Kinetic study revealed that <jats:styled-content style=\"fixed-case\">TSA<\/jats:styled-content>\u2010induced apoptosis was significantly dependent on enhanced transcription and protein synthesis in an early stage, which subsequently caused <jats:styled-content style=\"fixed-case\">ER<\/jats:styled-content> stress and apoptosis. Ch<jats:styled-content style=\"fixed-case\">IP<\/jats:styled-content> analysis indicated that <jats:styled-content style=\"fixed-case\">TSA<\/jats:styled-content> increased H4K16 acetylation, promoting <jats:styled-content style=\"fixed-case\">ER<\/jats:styled-content> stress gene transcription. The changes in Ac\u2010H4K16, <jats:styled-content style=\"fixed-case\">ATF<\/jats:styled-content>3 and <jats:styled-content style=\"fixed-case\">ATF<\/jats:styled-content>4 were also validated in <jats:styled-content style=\"fixed-case\">TSA<\/jats:styled-content>\u2010treated animals. Further study revealed the higher enzyme activity of <jats:styled-content style=\"fixed-case\">HDAC<\/jats:styled-content>s and an increase in acetylated proteins in resistant cells. The higher nucleocytoplasmic acetyl\u2010CoA in resistant cells was responsible for elevated acetylation status of protein and a more vigorous growth state. These results strongly support the pre\u2010clinical application of <jats:styled-content style=\"fixed-case\">HDAC<\/jats:styled-content> inhibitors for treating chemotherapy\u2010resistant <jats:styled-content style=\"fixed-case\">mCRPC<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/jcmm.13475","type":"journal-article","created":{"date-parts":[[2018,1,12]],"date-time":"2018-01-12T11:38:33Z","timestamp":1515757113000},"page":"1909-1922","source":"Crossref","is-referenced-by-count":24,"title":["Hyper\u2010acetylation contributes to the sensitivity of chemo\u2010resistant prostate cancer cells to histone deacetylase inhibitor Trichostatin A"],"prefix":"10.1111","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7703-5872","authenticated-orcid":false,"given":"Qingqing","family":"Xu","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Shandong University School of Medicine Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofei","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Shandong University School of Medicine Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shiqin","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Shandong University School of Medicine Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xuelei","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Shandong University School of Medicine Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huanmin","family":"Niu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Shandong University School of Medicine Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiulei","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Shandong University School of Medicine Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Deyu","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Shandong University School of Medicine Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Effat Un","family":"Nesa","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Shandong University School of Medicine Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Keli","family":"Tian","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Shandong University School of Medicine Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiqing","family":"Yuan","sequence":"additional","affiliation":[{"name":"Department of Biochemistry and Molecular Biology Shandong University School of Medicine Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,1,12]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1470-2045(15)70033-1"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrclinonc.2010.136"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2011.02.013"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2013.07.003"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.bjc.6604199"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bmcl.2003.10.055"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-394387-3.00007-0"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.10998"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-06-3996"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.12583"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejcb.2014.05.004"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2013.285"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2013.05.022"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-477-3_24"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(89)90625-9"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2005.03.051"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.182372299"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4402125"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI0216784"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST0340007"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1209888"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/cdd.2012.3"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ceb.2015.02.003"},{"key":"e_1_2_7_25_1","first-page":"273","article-title":"Screening of histone deacetylases (HDAC) expression in human prostate cancer reveals distinct class I HDAC profiles between epithelial and stromal cells","volume":"48","author":"Waltregny D","year":"2004","journal-title":"Eur J Histochem"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1158\/1535-7163.MCT-04-0287"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1177\/1756287215597637"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0145985"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.10327"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1186\/1476-4598-10-126"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/pros.20581"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00018-002-8498-3"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11060-008-9709-z"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2016.409"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2015.328"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2013.01.029"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ccr.2005.09.008"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST20140135"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1101\/sqb.2011.76.010769"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjcmm.13475","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.13475","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,24]],"date-time":"2023-09-24T12:59:52Z","timestamp":1695560392000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.13475"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,1,12]]},"references-count":38,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2018,3]]}},"alternative-id":["10.1111\/jcmm.13475"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.13475","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,1,12]]}}}