{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:08:17Z","timestamp":1787015297672,"version":"build-2736575974"},"reference-count":25,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,3,8]],"date-time":"2018-03-08T00:00:00Z","timestamp":1520467200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2018,5]]},"abstract":"Abstract<\/jats:title>\n \n This study aimed to explore the effects of\n CLIC<\/jats:styled-content>\n 1 gene silencing on proliferation, migration, invasion and apoptosis of human gallbladder cancer (\n GBC<\/jats:styled-content>\n ).\n GBC<\/jats:styled-content>\n and normal gallbladder tissues were extracted for the detection of\n mRNA<\/jats:styled-content>\n and protein expressions of\n CLIC<\/jats:styled-content>\n 1.\n GBC<\/jats:styled-content>\n \u2010\n SD<\/jats:styled-content>\n and\n NOZ<\/jats:styled-content>\n cells in the logarithmic growth phase were selected to conduct the experiment. Three different si\n RNA<\/jats:styled-content>\n recombined expression vectors were established using\n CLIC<\/jats:styled-content>\n 1 as a target at different sites. Reverse transcription quantitative polymerase chain reaction (\n RT<\/jats:styled-content>\n \u2010\n qPCR<\/jats:styled-content>\n ) and Western blotting were, respectively, used to detect the\n CLIC<\/jats:styled-content>\n 1\n mRNA<\/jats:styled-content>\n and protein expressions.\n MTT<\/jats:styled-content>\n assay was performed to detect the cell proliferation. Flow cytometry was applied to measure the cell apoptosis and cell cycle distribution. The variations of cell migration and invasion were evaluated using Transwell assay.\n GBC<\/jats:styled-content>\n tissues showed higher\n CLIC<\/jats:styled-content>\n 1\n mRNA<\/jats:styled-content>\n and protein expressions than normal gallbladder tissues. The\n CLIC<\/jats:styled-content>\n 1\n mRNA<\/jats:styled-content>\n and protein expressions in the\n CLIC<\/jats:styled-content>\n 1 si\n RNA<\/jats:styled-content>\n group were significantly lower than those in the\n NC<\/jats:styled-content>\n and blank groups. Compared with the\n NC<\/jats:styled-content>\n and blank groups, the\n CLIC<\/jats:styled-content>\n 1 si\n RNA<\/jats:styled-content>\n group showed a significant decrease in cell proliferation but an obvious increase in apoptosis rate in\n GBC<\/jats:styled-content>\n cells. Besides, in the\n CLIC<\/jats:styled-content>\n 1 si\n RNA<\/jats:styled-content>\n group, cell percentage in G0\/G1 and G2\/M phase was gradually increased but decreased in S phases. The migration and invasion abilities in\n GBC<\/jats:styled-content>\n cells were significantly lower than those in the\n NC<\/jats:styled-content>\n and blank groups. Our study demonstrates that\n CLIC<\/jats:styled-content>\n 1 gene silencing could promote apoptosis and inhibit proliferation migration and invasion of GBC cells.\n <\/jats:p>","DOI":"10.1111\/jcmm.13499","type":"journal-article","created":{"date-parts":[[2018,3,8]],"date-time":"2018-03-08T01:05:21Z","timestamp":1520471121000},"page":"2569-2579","source":"Crossref","is-referenced-by-count":18,"title":["Retracted:<\/i>\n Effect of\n CLIC<\/scp>\n 1 gene silencing on proliferation, migration, invasion and apoptosis of human gallbladder cancer cells"],"prefix":"10.1111","volume":"22","author":[{"given":"Yue\u2010Ming","family":"He","sequence":"first","affiliation":[{"name":"Department of Hepato\u2010Pancreato\u2010Biliary Surgery Zhongnan Hospital of Wuhan University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhong\u2010Lin","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Hepato\u2010Pancreato\u2010Biliary Surgery Zhongnan Hospital of Wuhan University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Quan\u2010Yan","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Hepato\u2010Pancreato\u2010Biliary Surgery Zhongnan Hospital of Wuhan University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Sha","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Hepato\u2010Pancreato\u2010Biliary Surgery Zhongnan Hospital of Wuhan University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Wei","sequence":"additional","affiliation":[{"name":"Department of Hepato\u2010Pancreato\u2010Biliary Surgery Zhongnan Hospital of Wuhan University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9064-4963","authenticated-orcid":false,"given":"Chen","family":"Xi","sequence":"additional","affiliation":[{"name":"College of Life Science Wuhan University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiang","family":"Nan","sequence":"additional","affiliation":[{"name":"College of Life Science Wuhan University Wuhan China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,3,8]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-016-4852-1"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11033-014-3428-7"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.14694\/EdBook_AM.2014.34.e165"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0039-6060(05)80072-8"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1155\/2005\/565479"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/annonc\/10.suppl_4.S129"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10616-014-9763-7"},{"key":"e_1_2_7_9_1","first-page":"72","article-title":"Inhibitory effect of norcantharidin on the growth of human gallbladder carcinoma GBC\u2010SD cells in\u00a0vitro","volume":"6","author":"Fan YZ","year":"2007","journal-title":"Hepatobiliary Pancreat Dis Int"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0166790"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djt278"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12032-015-0616-9"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.3892\/mmr.2016.4972"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2011.10.002"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-012-1271-5"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s13277-015-3052-8"},{"key":"e_1_2_7_17_1","first-page":"99","article-title":"Gallbladder cancer: epidemiology and outcome","volume":"6","author":"Hundal R","year":"2014","journal-title":"Clin Epidemiol"},{"key":"e_1_2_7_18_1","doi-asserted-by":"publisher","DOI":"10.1097\/IGC.0b013e3182022997"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v18.i24.3070"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v20.i8.2071"},{"key":"e_1_2_7_21_1","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.2431-08.2008"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2007.06145.x"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1021\/acs.biochem.6b00080"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0074523"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/3928714"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00249-009-0450-0"}],"updated-by":[{"DOI":"10.1111\/jcmm.16517","type":"retraction","label":"Retraction","source":"retraction-watch","updated":{"date-parts":[[2021,5,4]],"date-time":"2021-05-04T00:00:00Z","timestamp":1620086400000},"record-id":"28320"},{"DOI":"10.1111\/jcmm.16517","type":"retraction","label":"Retraction","source":"publisher","updated":{"date-parts":[[2021,5,4]],"date-time":"2021-05-04T00:00:00Z","timestamp":1620086400000}}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjcmm.13499","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.13499","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jcmm.13499","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.13499","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,1]],"date-time":"2023-09-01T14:48:00Z","timestamp":1693579680000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.13499"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,3,8]]},"references-count":25,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2018,5]]}},"alternative-id":["10.1111\/jcmm.13499"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.13499","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,3,8]]}}}