{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,19]],"date-time":"2026-09-19T08:56:03Z","timestamp":1789808163696,"version":"4.0.1"},"reference-count":41,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,2,7]],"date-time":"2018-02-07T00:00:00Z","timestamp":1517961600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004462","name":"Consiglio Nazionale delle Ricerche","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004462","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005075","name":"Universitair Medisch Centrum Groningen","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005075","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2018,4]]},"abstract":"Abstract<\/jats:title>\n \n Inflammation and ageing are intertwined in chronic obstructive pulmonary disease (\n COPD<\/jats:styled-content>\n ). The histone deacetylase\n SIRT<\/jats:styled-content>\n 1 and the related activation of FoxO3 protect from ageing and regulate inflammation. The role of\n SIRT<\/jats:styled-content>\n 1\/FoxO3 in\n COPD<\/jats:styled-content>\n is largely unknown. This study evaluated whether cigarette smoke, by modulating the\n SIRT<\/jats:styled-content>\n 1\/FoxO3 axis, affects airway epithelial pro\u2010inflammatory responses. Human bronchial epithelial cells (16\n HBE<\/jats:styled-content>\n ) and primary bronchial epithelial cells (\n PBEC<\/jats:styled-content>\n s) from\n COPD<\/jats:styled-content>\n patients and controls were treated with\/without cigarette smoke extract (\n CSE<\/jats:styled-content>\n ), Sirtinol or FoxO3 si\n RNA<\/jats:styled-content>\n .\n SIRT<\/jats:styled-content>\n 1, FoxO3 and\n NF<\/jats:styled-content>\n \u2010\u03baB nuclear accumulation,\n SIRT<\/jats:styled-content>\n 1 deacetylase activity,\n IL<\/jats:styled-content>\n \u20108 and\n CCL<\/jats:styled-content>\n 20 expression\/release and the release of 12 cytokines, neutrophil and lymphocyte chemotaxis were assessed. In\n PBEC<\/jats:styled-content>\n s, the constitutive FoxO3 expression was lower in patients with\n COPD<\/jats:styled-content>\n than in controls. Furthermore,\n CSE<\/jats:styled-content>\n reduced FoxO3 expression only in\n PBEC<\/jats:styled-content>\n s from controls. In 16\n HBE<\/jats:styled-content>\n ,\n CSE<\/jats:styled-content>\n decreased\n SIRT<\/jats:styled-content>\n 1 activity and nuclear expression, enhanced\n NF<\/jats:styled-content>\n \u2010\u03baB binding to the\n IL<\/jats:styled-content>\n \u20108 gene promoter thus increasing\n IL<\/jats:styled-content>\n \u20108 expression, decreased\n CCL<\/jats:styled-content>\n 20 expression, increased the neutrophil chemotaxis and decreased lymphocyte chemotaxis. Similarly,\n SIRT<\/jats:styled-content>\n 1 inhibition reduced FoxO3 expression and increased nuclear\n NF<\/jats:styled-content>\n \u2010\u03baB. FoxO3 si\n RNA<\/jats:styled-content>\n treatment increased\n IL<\/jats:styled-content>\n \u20108 and decreased\n CCL<\/jats:styled-content>\n 20 expression in 16\n HBE<\/jats:styled-content>\n . In conclusion,\n CSE<\/jats:styled-content>\n impairs the function of\n SIRT<\/jats:styled-content>\n 1\/FoxO3 axis in bronchial epithelium, dysregulating\n NF<\/jats:styled-content>\n \u2010\u03baB activity and inducing pro\u2010inflammatory responses.\n <\/jats:p>","DOI":"10.1111\/jcmm.13509","type":"journal-article","created":{"date-parts":[[2018,2,7]],"date-time":"2018-02-07T04:02:38Z","timestamp":1517976158000},"page":"2272-2282","source":"Crossref","is-referenced-by-count":56,"title":["SIRT<\/scp>\n 1\/FoxO3 axis alteration leads to aberrant immune responses in bronchial epithelial cells"],"prefix":"10.1111","volume":"22","author":[{"given":"Serena","family":"Di Vincenzo","sequence":"first","affiliation":[{"name":"Istituto di Biomedicina e Immunologia Molecolare\u2010Consiglio Nazionale delle Ricerche Palermo Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irene H.","family":"Heijink","sequence":"additional","affiliation":[{"name":"Department of Pathology 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