{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T19:49:30Z","timestamp":1773776970840,"version":"3.50.1"},"reference-count":55,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,3,13]],"date-time":"2018-03-13T00:00:00Z","timestamp":1520899200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Gamlatj\u00e4rnarinor"},{"name":"Gun and Bertil Stohne's"},{"name":"Olle Engkvist Byggm\u00e4stare Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2018,6]]},"abstract":"Abstract<\/jats:title>Cerebral autosomal\u2010dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL<\/jats:styled-content>) is a familial fatal progressive degenerative disorder. One of the pathological hallmarks of CADASIL<\/jats:styled-content> is a dramatic reduction of vascular smooth muscle cells (VSMC<\/jats:styled-content>s) in cerebral arteries. Using VSMC<\/jats:styled-content>s from the vasculature of the human umbilical cord, placenta and cerebrum of CADASIL<\/jats:styled-content> patients, we found that CADASIL VSMC<\/jats:styled-content>s had a lower proliferation rate compared to control VSMC<\/jats:styled-content>s. Exposure of control VSMC<\/jats:styled-content>s and endothelial cells (EC<\/jats:styled-content>s) to media derived from CADASIL VSMC<\/jats:styled-content>s lowered the proliferation rate of all cells examined. By quantitative RT<\/jats:styled-content>\u2010PCR<\/jats:styled-content> analysis, we observed increased Transforming growth factor\u2010\u03b2 (TGF<\/jats:styled-content>\u03b2) gene expression in CADASIL VSMC<\/jats:styled-content>s. Adding TGF<\/jats:styled-content>\u03b2\u2010neutralizing antibody restored the proliferation rate of CADASIL VSMC<\/jats:styled-content>s. We assessed proliferation differences in the presence or absence of TGF<\/jats:styled-content>\u03b2\u2010neutralizing antibody in EC<\/jats:styled-content>s co\u2010cultured with VSMC<\/jats:styled-content>s. EC<\/jats:styled-content>s co\u2010cultured with CADASIL VSMC<\/jats:styled-content>s exhibited a lower proliferation rate than those co\u2010cultured with control VSMC<\/jats:styled-content>s, and neutralization of TGF<\/jats:styled-content>\u03b2 normalized the proliferation rate of EC<\/jats:styled-content>s co\u2010cultured with CADASIL VSMC<\/jats:styled-content>s. We suggest that increased TGF<\/jats:styled-content>\u03b2 expression in CADASIL VSMC<\/jats:styled-content>s is involved in the reduced VSMC<\/jats:styled-content> proliferation in CADASIL<\/jats:styled-content> and may play a role in situ in altered proliferation of neighbouring cells in the vasculature.<\/jats:p>","DOI":"10.1111\/jcmm.13534","type":"journal-article","created":{"date-parts":[[2018,3,15]],"date-time":"2018-03-15T07:32:16Z","timestamp":1521099136000},"page":"3016-3024","source":"Crossref","is-referenced-by-count":16,"title":["Differences in proliferation rate between CADASIL<\/scp> and control vascular smooth muscle cells are related to increased TGF<\/scp>\u03b2 expression"],"prefix":"10.1111","volume":"22","author":[{"given":"Mahmod","family":"Panahi","sequence":"first","affiliation":[{"name":"Karolinska Institute Department of Neurobiology, Care Sciences and Society Division of Neurogeriatrics Center for Alzheimer Research Huddinge Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naeimeh","family":"Yousefi Mesri","sequence":"additional","affiliation":[{"name":"Karolinska Institute Department of Neurobiology, Care Sciences and Society Division of Neurogeriatrics Center for Alzheimer Research Huddinge Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eva\u2010Britt","family":"Samuelsson","sequence":"additional","affiliation":[{"name":"Division of Neurodegeneration Huddinge Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kirsten G.","family":"Coupland","sequence":"additional","affiliation":[{"name":"Karolinska Institute Department of Neurobiology, Care Sciences and Society Division of Neurogeriatrics Center for Alzheimer Research Huddinge Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charlotte","family":"Forsell","sequence":"additional","affiliation":[{"name":"Karolinska Institute Department of Neurobiology, Care Sciences and Society Division of Neurogeriatrics Center for Alzheimer Research Huddinge Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Caroline","family":"Graff","sequence":"additional","affiliation":[{"name":"Karolinska Institute Department of Neurobiology, Care Sciences and Society Division of Neurogeriatrics Center for Alzheimer Research Huddinge Sweden"},{"name":"Department of Geriatric Medicine Genetics Unit Karolinska University Hospital Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Saara","family":"Tikka","sequence":"additional","affiliation":[{"name":"Medicum Biochemistry\/Developmental Biology Meilahti Clinical Proteomics Core Facility University of Helsinki Helsinki Finland"},{"name":"Folkh\u00e4lsan Institute of Genetics Helsinki Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bengt","family":"Winblad","sequence":"additional","affiliation":[{"name":"Karolinska Institute Department of Neurobiology, Care Sciences and Society Division of Neurogeriatrics Center for Alzheimer Research Huddinge Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Matti","family":"Viitanen","sequence":"additional","affiliation":[{"name":"Department of Geriatrics Turun Kaupunginsairaala University Hospital of Turku University of Turku Turku Finland"},{"name":"Karolinska Institute Department of Neurobiology, Care Sciences and Society Division of Clinical Geriatrics Karolinska University Hospital Huddinge Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helena","family":"Karlstr\u00f6m","sequence":"additional","affiliation":[{"name":"Karolinska Institute Department of Neurobiology, Care Sciences and Society Division of Neurogeriatrics Center for Alzheimer Research Huddinge Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Erik","family":"Sundstr\u00f6m","sequence":"additional","affiliation":[{"name":"Division of Neurodegeneration Huddinge Sweden"},{"name":"Stockholms Sjukhem R&D unit Stockholm Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2612-0858","authenticated-orcid":false,"given":"Homira","family":"Behbahani","sequence":"additional","affiliation":[{"name":"Karolinska Institute Department of Neurobiology, Care Sciences and Society Division of Neurogeriatrics Center for Alzheimer Research Huddinge Sweden"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,3,13]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(09)70127-9"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(97)08083-5"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.51.2.452"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(95)91557-5"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.30.6.1230"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410440506"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1212\/01.wnl.0000344166.03470.f8"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI8047"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-3639.2002.tb00451.x"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M202224200"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1097\/nen.0b013e318093e574"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.STR.25.11.2291"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00296354"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-3639.2004.tb00078.x"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.45.6.1086"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.1997.tb48463.x"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.063610"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.284.5415.770"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/bpa.12181"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.57.9.1714"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ejhg.5200460"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1001\/archneur.61.6.942"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.54.9.1874"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1001\/archneur.62.7.1091"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/bpa.12177"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0b013e3182407968"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1161\/hs0102.100885"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jstrokecerebrovasdis.2016.08.007"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2012.09.015"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.20558"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1186\/s40478-014-0096-8"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1124\/mol.107.039602"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cardiores.2004.10.036"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.2119\/2006-00069.Ihalainen"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/jcbfm.2012.123"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2004.13.337"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1350705"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1749-6632.2000.tb06377.x"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-3639.2004.tb00497.x"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(00)00418-0"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.86.2.913"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpcell.00048.2001"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10620-014-3439-1"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bjps.2005.04.009"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1101964108"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1789.2011.01290.x"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.115.003042"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexcr.2013.02.015"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.20311"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.1096\/fasebj.14.1.55"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600386"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M503471200"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12012-015-9341-z"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-017-00204-4"},{"key":"e_1_2_8_56_1","doi-asserted-by":"publisher","DOI":"10.1186\/bcr43"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjcmm.13534","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.13534","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jcmm.13534","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.13534","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,15]],"date-time":"2025-10-15T07:00:20Z","timestamp":1760511620000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.13534"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,3,13]]},"references-count":55,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2018,6]]}},"alternative-id":["10.1111\/jcmm.13534"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.13534","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,3,13]]}}}