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This study aimed to further elucidate the pro\u2010angiogenic effect and mechanism ofHSYA<\/jats:styled-content>on ischaemic cardiac dysfunction. A C57 mouse model of acute myocardial infarction (AMI<\/jats:styled-content>) was firstly established, and 25\u00a0mg\/kgHSYA<\/jats:styled-content>was intraperitoneally injected immediately after operation and given once, respectively, each morning and evening for 2\u00a0weeks. It was found thatHSYA<\/jats:styled-content>significantly improved ischaemia\u2010induced cardiac haemodynamics, enhanced the survival rate, alleviated the myocardial injury and increased the expressions ofCD<\/jats:styled-content>31, vascular endothelial growth factor\u2010A (VEGF<\/jats:styled-content>\u2010A) and nucleolin in the ischaemic myocardium. In addition,HSYA<\/jats:styled-content>promoted the migration and tube formation of human umbilical vein endothelial cells (HUVEC<\/jats:styled-content>s), enhanced the expressions of nucleolin,VEGF<\/jats:styled-content>\u2010A and matrix metalloproteinase\u20109 (MMP<\/jats:styled-content>\u20109) in a dose\u2010 and time\u2010dependent manner. However, down\u2010regulation of nucleolin expression sharply abrogated the effect mentioned above ofHSYA<\/jats:styled-content>. Further protein\u2010RNA<\/jats:styled-content>coimmunoprecipitation and immunoprecipitation\u2010RT<\/jats:styled-content>\u2010PCR<\/jats:styled-content>assay showed that nucleolin binded toVEGF<\/jats:styled-content>\u2010A andMMP<\/jats:styled-content>\u20109mRNA<\/jats:styled-content>and overexpression of nucleolin up\u2010regulated themRNA<\/jats:styled-content>expressions ofVEGF<\/jats:styled-content>\u2010A andMMP<\/jats:styled-content>\u20109 in theHUVEC<\/jats:styled-content>s through enhancing the stability ofVEGF<\/jats:styled-content>\u2010A andMMP<\/jats:styled-content>\u20109mRNA<\/jats:styled-content>. Furthermore,HSYA<\/jats:styled-content>increased themRNA<\/jats:styled-content>expressions ofVEGF<\/jats:styled-content>\u2010A andMMP<\/jats:styled-content>\u20109 in the extract of antinucleolin antibody\u2010precipitated protein from the heart ofAMI<\/jats:styled-content>mice. Our data revealed that nucleolin mediated the pro\u2010angiogenic effect ofHSYA<\/jats:styled-content>through post\u2010transcriptional regulation ofVEGF<\/jats:styled-content>\u2010A andMMP<\/jats:styled-content>\u20109 expression, which contributed to the protective effect ofHSYA<\/jats:styled-content>on ischaemic cardiac dysfunction.<\/jats:p>","DOI":"10.1111\/jcmm.13552","type":"journal-article","created":{"date-parts":[[2018,3,7]],"date-time":"2018-03-07T12:52:41Z","timestamp":1520427161000},"page":"2692-2705","source":"Crossref","is-referenced-by-count":54,"title":["Nucleolin mediated pro\u2010angiogenic role of Hydroxysafflor Yellow A in ischaemic cardiac dysfunction: Post\u2010transcriptional regulation ofVEGF<\/scp>\u2010A andMMP<\/scp>\u20109"],"prefix":"10.1111","volume":"22","author":[{"given":"Jiang","family":"Zou","sequence":"first","affiliation":[{"name":"Department of Pathophysiology Xiangya School of Medicine Central South University Changsha 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