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Here, we hypothesize that the in\u00a0vitro exposure of human hypopharyngeal cells to acidic bile deregulates cancer\u2010related mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> markers that can be reversed by <jats:styled-content style=\"fixed-case\">BAY<\/jats:styled-content> 11\u20107082, a pharmacologic <jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>\u2010\u03baB inhibitor. We repetitively exposed normal human hypopharyngeal primary cells and human hypopharyngeal keratinocytes to bile fluid (400\u00a0\u03bcmol\/L), at <jats:styled-content style=\"fixed-case\">pH<\/jats:styled-content> 4.0 and 7.0, with\/without <jats:styled-content style=\"fixed-case\">BAY<\/jats:styled-content> 11\u20107082 (20\u00a0\u03bcmol\/L). We centred our study on the transcriptional activation of oncogenic miR\u201021, miR\u2010155, miR\u2010192, miR\u201034a, miR\u2010375, miR\u2010451a and <jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>\u2010\u03baB\u2010related genes, previously linked to acidic bile\u2010induced pre\u2010neoplastic events. Our novel findings in\u00a0vitro are consistent with our hypothesis demonstrating that <jats:styled-content style=\"fixed-case\">BAY<\/jats:styled-content> 11\u20107082 significantly reverses the acidic bile\u2010induced oncogenic mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> phenotype, in normal hypopharyngeal cells. <jats:styled-content style=\"fixed-case\">BAY<\/jats:styled-content> 11\u20107082 strongly inhibits the acidic bile\u2010induced up\u2010regulation of miR\u2010192 and down\u2010regulation of miR\u2010451a and significantly decreases the miR\u201021\/375 ratios, previously related to poor prognosis in hypopharyngeal cancer. This is the first in\u00a0vitro report that <jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>\u2010\u03baB inhibition reverses acidic bile\u2010induced miR\u201021, miR\u2010155, miR\u2010192, miR\u201034a, miR\u2010375 and miR\u2010451a deregulations in normal human hypopharyngeal cells, suggesting that acidic bile\u2010induced events are directly or indirectly dependent on <jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>\u2010\u03baB signalling.<\/jats:p>","DOI":"10.1111\/jcmm.13591","type":"journal-article","created":{"date-parts":[[2018,3,8]],"date-time":"2018-03-08T06:23:24Z","timestamp":1520490204000},"page":"2922-2934","source":"Crossref","is-referenced-by-count":27,"title":["<scp>NF<\/scp>\u2010\u03baB inhibition reverses acidic bile\u2010induced miR\u201021, miR\u2010155, miR\u2010192, miR\u201034a, miR\u2010375 and miR\u2010451a deregulations in human hypopharyngeal cells"],"prefix":"10.1111","volume":"22","author":[{"given":"Sotirios 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