{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,23]],"date-time":"2026-06-23T22:56:33Z","timestamp":1782255393211,"version":"3.54.5"},"reference-count":32,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,3,24]],"date-time":"2018-03-24T00:00:00Z","timestamp":1521849600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005795","name":"Chang Gung Memorial Hospital, Linkou","doi-asserted-by":"publisher","award":["CMRP No. CMRPG3G1661"],"award-info":[{"award-number":["CMRP No. CMRPG3G1661"]}],"id":[{"id":"10.13039\/501100005795","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01 CA140654"],"award-info":[{"award-number":["R01 CA140654"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2018,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Although tumour PD\u2010L1 (CD274) expression had been used as a predictive biomarker in checkpoint immunotherapy targeting the PD1\/PD\u2010L1 axis in various cancers, the regulation of PD\u2010L1 (CD274) expression is unclear. Yes\u2010associated protein (YAP), an important oncogenic protein in Hippo signalling pathway, reportedly promotes cancer development. We investigated whether inhibition of YAP down\u2010regulates PD\u2010L1 (CD274) in human malignant pleural mesothelioma (MPM). Western blotting showed that 2 human MPM cell lines (H2052 and 211H) had increased PD\u2010L1 protein expression compared to H290, MS\u20101 and H28 cells. In H2052 and 211H cells, PD\u2010L1 mRNA expression was significantly increased compared to other MPM cell lines; YAP knockdown by small interfering RNA decreased PD\u2010L1 protein and mRNA expression. Forced overexpression of the YAP gene increased PD\u2010L1 protein expression in H2452 cells. Chromatin immunoprecipitation (ChIP) assay showed the precipitation of PD\u2010L1 enhancer region encompassing 2 putative YAP\u2010TEAD\u2010binding sites in H2052 cells. We found that, in human MPM tissue microarray samples, YAP and PD\u2010L1 concurrently expressed in immunohistochemistry stain (n\u00a0=\u00a070, <jats:italic>P<\/jats:italic>\u00a0&lt;\u00a0.05, chi\u2010square). We conclude that PD\u2010L1 is correlated with YAP expression, and inhibition of YAP down\u2010regulates PD\u2010L1 expression in human MPM. Further study of how YAP regulates PD\u2010L1 in MPM is warranted.<\/jats:p>","DOI":"10.1111\/jcmm.13593","type":"journal-article","created":{"date-parts":[[2018,3,25]],"date-time":"2018-03-25T02:35:11Z","timestamp":1521945311000},"page":"3139-3148","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":49,"title":["Inhibition of yes\u2010associated protein down\u2010regulates PD\u2010L1 (CD274) expression in human malignant pleural mesothelioma"],"prefix":"10.1111","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0173-7509","authenticated-orcid":false,"given":"Ping\u2010Chih","family":"Hsu","sequence":"first","affiliation":[{"name":"Department of Surgery Helen Diller Family Comprehensive Cancer Center University of California, San Francisco San Francisco CA USA"},{"name":"Department of Thoracic Medicine Chang Gung Memorial Hospital, Linkou Taoyuan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinbai","family":"Miao","sequence":"additional","affiliation":[{"name":"Department of Surgery Helen Diller Family Comprehensive Cancer Center University of California, San Francisco San Francisco CA USA"},{"name":"Department of Thoracic Surgery Beijing Chao\u2010Yang Hospital Affiliated with Capital Medical University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yu\u2010Cheng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Surgery Helen Diller Family Comprehensive Cancer Center University of California, San Francisco San Francisco CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wen\u2010Qian","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Surgery Helen Diller Family Comprehensive Cancer Center University of California, San Francisco San Francisco CA USA"},{"name":"Department of Thoracic Surgery Beijing Chao\u2010Yang Hospital Affiliated with Capital Medical University Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi\u2010Lin","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Surgery Helen Diller Family Comprehensive Cancer Center University of California, San Francisco San Francisco CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chih\u2010Wei","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathology Chang Gung Memorial Hospital, Linkou Taoyuan Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cheng\u2010Ta","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Surgery Helen Diller Family Comprehensive Cancer Center University of California, San Francisco San Francisco CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery National Cancer Center\/Cancer Hospital Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joanna","family":"You","sequence":"additional","affiliation":[{"name":"Department of Surgery Helen Diller Family Comprehensive Cancer Center University of California, San Francisco San Francisco CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhidong","family":"Xu","sequence":"additional","affiliation":[{"name":"Department of Surgery Helen Diller Family Comprehensive Cancer Center University of California, San Francisco San Francisco CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David M.","family":"Jablons","sequence":"additional","affiliation":[{"name":"Department of Surgery 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