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Micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010200b (miR\u2010200b) has been reported as a tumour suppressor, but its role in the precancerous <jats:styled-content style=\"fixed-case\">OSF<\/jats:styled-content> remains unknown. In this study, we investigated the impact of miR\u2010200b on myofibroblastic differentiation activity. Arecoline is a major areca nut alkaloid and has been employed to induce the elevated myofibroblast activity in human buccal mucosal fibroblasts (<jats:styled-content style=\"fixed-case\">BMF<\/jats:styled-content>s). Treatment of arecoline in <jats:styled-content style=\"fixed-case\">BMF<\/jats:styled-content>s dose\u2010dependently reduced gene expression of miR\u2010200b, which corresponded with the decreased expression of miR\u2010200b in <jats:styled-content style=\"fixed-case\">fBMF<\/jats:styled-content>s. The arecoline\u2010induced myofibroblast activities were abolished by overexpression of miR\u2010200b in <jats:styled-content style=\"fixed-case\">BMF<\/jats:styled-content>s, and the same results were observed in <jats:styled-content style=\"fixed-case\">fBMF<\/jats:styled-content>s. In addition, \u03b1\u2010<jats:styled-content style=\"fixed-case\">SMA<\/jats:styled-content> was inhibited by an increase in miR\u2010200b. We further demonstrated that miR\u2010200b\u2010mediated decrease in <jats:styled-content style=\"fixed-case\">ZEB<\/jats:styled-content>2 led to down\u2010regulation of \u03b1\u2010<jats:styled-content style=\"fixed-case\">SMA<\/jats:styled-content>, vimentin. Loss of miR\u2010200b resulted in enhanced collagen contraction and migration capabilities, and knockdown of <jats:styled-content style=\"fixed-case\">ZEB<\/jats:styled-content>2 reversed these phenomena. Lastly, we showed the expression of miR\u2010200b was significantly less and <jats:styled-content style=\"fixed-case\">ZEB<\/jats:styled-content>2 was markedly higher in <jats:styled-content style=\"fixed-case\">OSF<\/jats:styled-content> tissues. These results suggested that down\u2010regulation of miR\u2010200b may contribute to the pathogenesis of areca quid\u2010associated <jats:styled-content style=\"fixed-case\">OSF<\/jats:styled-content> through the regulation of <jats:styled-content style=\"fixed-case\">ZEB<\/jats:styled-content>2 and myofibroblast hallmarks.<\/jats:p>","DOI":"10.1111\/jcmm.13690","type":"journal-article","created":{"date-parts":[[2018,6,12]],"date-time":"2018-06-12T09:08:51Z","timestamp":1528794531000},"page":"4130-4138","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":42,"title":["miR\u2010200b ameliorates myofibroblast transdifferentiation in precancerous oral submucous fibrosis through targeting <scp>ZEB<\/scp>2"],"prefix":"10.1111","volume":"22","author":[{"given":"Yi\u2010Wen","family":"Liao","sequence":"first","affiliation":[{"name":"School of Dentistry Chung Shan Medical University Taichung 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