{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T10:36:03Z","timestamp":1769164563251,"version":"3.49.0"},"reference-count":60,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2018,7,11]],"date-time":"2018-07-11T00:00:00Z","timestamp":1531267200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["NIH\/R01 #112029"],"award-info":[{"award-number":["NIH\/R01 #112029"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Pulmonary angiogenesis is essential for alveolarization, the final stage of lung development that markedly increases gas exchange surface area. We recently demonstrated that activation of the nuclear factor kappa\u2010B (NF\u03baB) pathway promotes pulmonary angiogenesis during alveolarization. However, the mechanisms activating NF\u03baB in the pulmonary endothelium, and its downstream targets are not known. In this study, we sought to delineate the specific roles for the NF\u03baB activating kinases, IKK\u03b1 and IKK\u03b2, in promoting developmental pulmonary angiogenesis. Microarray analysis of primary pulmonary endothelial cells (PECs) after silencing IKK\u03b1 or IKK\u03b2 demonstrated that the 2 kinases regulate unique panels of genes, with few shared targets. Although silencing IKK\u03b1 induced mild impairments in angiogenic function, silencing IKK\u03b2 induced more severe angiogenic defects and decreased vascular cell adhesion molecule expression, an IKK\u03b2 regulated target essential for both PEC adhesion and migration. Taken together, these data show that IKK\u03b1 and IKK\u03b2 regulate unique genes in PEC, resulting in differential effects on angiogenesis upon inhibition, and identify IKK\u03b2 as the predominant regulator of pulmonary angiogenesis during alveolarization. These data suggest that therapeutic strategies to specifically enhance IKK\u03b2 activity in the pulmonary endothelium may hold promise to enhance lung growth in diseases marked by altered alveolarization.<\/jats:p>","DOI":"10.1111\/jcmm.13741","type":"journal-article","created":{"date-parts":[[2018,7,11]],"date-time":"2018-07-11T09:26:26Z","timestamp":1531301186000},"page":"4410-4422","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Distinct roles for I\u03baB kinases alpha and beta in regulating pulmonary endothelial angiogenic function during late lung development"],"prefix":"10.1111","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0535-8605","authenticated-orcid":false,"given":"Cristiana","family":"Iosef","sequence":"first","affiliation":[{"name":"Department of Pharmacology Faculty of Medicine University of Nevada Reno Reno NV USA"},{"name":"Department of Pediatrics Center for Excellence in 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