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Gypenosides<\/jats:italic> (Gps<\/jats:italic>), the major ingredients of Gynostemma pentaphylla<\/jats:italic> (Thunb<\/jats:italic>.) Makino<\/jats:italic>, have exerted the properties of anti\u2010hyperglycaemia and anti\u2010inflammation, but whether Gps<\/jats:italic> improve myocardial damage and the mechanism remains unclear. Here, we found that high glucose (HG<\/jats:styled-content>) induced myocardial damage by activating the NLRP<\/jats:styled-content>3 inflammasome and then promoting IL<\/jats:styled-content>\u20101\u03b2 and IL<\/jats:styled-content>\u201018 secretion in H9C2 cells and NRVM<\/jats:styled-content>s. Meanwhile, HG<\/jats:styled-content> elevated the production of ROS<\/jats:styled-content>, which was vital to NLRP<\/jats:styled-content>3 inflammasome activation. Moreover, the ROS<\/jats:styled-content> activated the NLRP<\/jats:styled-content>3 inflammasome mainly by cytochrome c<\/jats:italic> influx into the cytoplasm and binding to NLRP<\/jats:styled-content>3. Inhibition of ROS<\/jats:styled-content> and cytochrome c<\/jats:italic> dramatically down\u2010regulated NLRP<\/jats:styled-content>3 inflammasome activation and improved the cardiomyocyte damage induced by HG<\/jats:styled-content>, which was also detected in cells treated by Gps<\/jats:italic>. Furthermore, Gps<\/jats:italic> also reduced the levels of the C\u2010reactive proteins (CRP<\/jats:styled-content>s), IL<\/jats:styled-content>\u20101\u03b2 and IL<\/jats:styled-content>\u201018, inhibited NLRP<\/jats:styled-content>3 inflammasome activation and consequently improved myocardial damage in\u00a0vivo. These findings provide a mechanism that ROS<\/jats:styled-content> induced by HG<\/jats:styled-content> activates the NLRP<\/jats:styled-content>3 inflammasome by cytochrome c<\/jats:italic> binding to NLRP<\/jats:styled-content>3 and that Gps<\/jats:italic> may be potential and effective drugs for DCM<\/jats:styled-content> via the inhibition of ROS<\/jats:styled-content>\u2010mediated NLRP<\/jats:styled-content>3 inflammasome activation.<\/jats:p>","DOI":"10.1111\/jcmm.13743","type":"journal-article","created":{"date-parts":[[2018,7,11]],"date-time":"2018-07-11T10:52:51Z","timestamp":1531306371000},"page":"4437-4448","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":138,"title":["Gypenosides improve diabetic cardiomyopathy by inhibiting ROS<\/scp>\u2010mediated NLRP<\/scp>3 inflammasome 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