{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T00:34:52Z","timestamp":1790814892380,"version":"4.1.0"},"reference-count":44,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2018,7,11]],"date-time":"2018-07-11T00:00:00Z","timestamp":1531267200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31600806"],"award-info":[{"award-number":["31600806"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81303286"],"award-info":[{"award-number":["81303286"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2018,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">IR<\/jats:styled-content>\u2010783 is a kind of heptamethine cyanine dye that exhibits imaging, cancer targeting and anticancer properties. A previous study reported that its imaging and targeting properties were related to mitochondria. However, the molecular mechanism behind the anticancer activity of <jats:styled-content style=\"fixed-case\">IR<\/jats:styled-content>\u2010783 has not been well demonstrated. In this study, we showed that <jats:styled-content style=\"fixed-case\">IR<\/jats:styled-content>\u2010783 inhibits cell viability and induces mitochondrial apoptosis in human breast cancer cells. Exposure of <jats:styled-content style=\"fixed-case\">MDA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MB<\/jats:styled-content>\u2010231 cells to <jats:styled-content style=\"fixed-case\">IR<\/jats:styled-content>\u2010783 resulted in the loss of mitochondrial membrane potential (<jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>), adenosine triphosphate (<jats:styled-content style=\"fixed-case\">ATP<\/jats:styled-content>) depletion, mitochondrial permeability transition pore (<jats:styled-content style=\"fixed-case\">mPTP<\/jats:styled-content>) opening and cytochrome c (Cyto C) release. Furthermore, we found that <jats:styled-content style=\"fixed-case\">IR<\/jats:styled-content>\u2010783 induced dynamin\u2010related protein 1 (Drp1) translocation from the cytosol to the mitochondria, increased the expression of mitochondrial fission proteins mitochondrial fission factor (<jats:styled-content style=\"fixed-case\">MFF<\/jats:styled-content>) and fission\u20101 (Fis1), and decreased the expression of mitochondrial fusion proteins mitofusin1 (Mfn1) and optic atrophy 1 (<jats:styled-content style=\"fixed-case\">OPA<\/jats:styled-content>1). Moreover, knockdown of Drp1 markedly blocked <jats:styled-content style=\"fixed-case\">IR<\/jats:styled-content>\u2010783\u2010mediated mitochondrial fission, loss of <jats:styled-content style=\"fixed-case\">MMP<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> ATP<\/jats:styled-content> depletion, <jats:styled-content style=\"fixed-case\">mPTP<\/jats:styled-content> opening and apoptosis. Our in\u00a0vivo study confirmed that <jats:styled-content style=\"fixed-case\">IR<\/jats:styled-content>\u2010783 markedly inhibited tumour growth and induced apoptosis in an <jats:styled-content style=\"fixed-case\">MDA<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">MB<\/jats:styled-content>\u2010231 xenograft model in association with the mitochondrial translocation of Drp1. Taken together, these findings suggest that <jats:styled-content style=\"fixed-case\">IR<\/jats:styled-content>\u2010783 induces apoptosis in human breast cancer cells by increasing Drp1\u2010mediated mitochondrial fission. Our study uncovered the molecular mechanism of the anti\u2010breast cancer effects of <jats:styled-content style=\"fixed-case\">IR<\/jats:styled-content>\u2010783 and provided novel perspectives for the application of <jats:styled-content style=\"fixed-case\">IR<\/jats:styled-content>\u2010783 in the treatment of breast cancer.<\/jats:p>","DOI":"10.1111\/jcmm.13749","type":"journal-article","created":{"date-parts":[[2018,7,11]],"date-time":"2018-07-11T12:02:47Z","timestamp":1531310567000},"page":"4474-4485","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":59,"title":["Dynamin\u2010related protein 1\u2010mediated mitochondrial fission contributes to <scp>IR<\/scp>\u2010783\u2010induced apoptosis in human breast cancer cells"],"prefix":"10.1111","volume":"22","author":[{"given":"Qin","family":"Tang","sequence":"first","affiliation":[{"name":"Department of Pharmacy The Second Affiliated Hospital of Army Medical University 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