{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T01:09:25Z","timestamp":1787015365235,"version":"build-2736575974"},"reference-count":25,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2018,7,5]],"date-time":"2018-07-05T00:00:00Z","timestamp":1530748800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100008530","name":"European Regional Development Fund","doi-asserted-by":"publisher","award":["A1.1.4. P_37_798, Ctr. 149\/26.10.2016, SMIS 106774"],"award-info":[{"award-number":["A1.1.4. 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Variations in circulating proteins might reflect local disease processes and plasma proteome profiling could serve to identify possible diagnostic and prognostic biomarkers. We employed a human cytokine array to screen for 105 distinct analytes in pooled plasma samples obtained from untreated young\n                    <jats:styled-content style=\"fixed-case\">MPN<\/jats:styled-content>\n                    patients (&lt;35\u00a0years) with different clinical phenotypes and driver mutations, as well as from healthy individuals. Among molecules that exhibited significantly increased levels in\n                    <jats:styled-content style=\"fixed-case\">MPN<\/jats:styled-content>\n                    patients versus controls, the top of the list was represented by Dickkopf\u2010related protein 1 (Dkk\u20101), which also showed the highest potential for discrimination between\n                    <jats:styled-content style=\"fixed-case\">MPN<\/jats:styled-content>\n                    subtypes. In the next step, a quantitative\n                    <jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>\n                    was used to measure plasma Dkk\u20101 levels in 30 young\u2010onset\n                    <jats:styled-content style=\"fixed-case\">MPN<\/jats:styled-content>\n                    \u201410 essential thrombocythemia (\n                    <jats:styled-content style=\"fixed-case\">ET<\/jats:styled-content>\n                    ), 10 polycythemia vera (\n                    <jats:styled-content style=\"fixed-case\">PV<\/jats:styled-content>\n                    ), 10 pre\u2010fibrotic primary myelofibrosis (pre\u2010\n                    <jats:styled-content style=\"fixed-case\">PMF<\/jats:styled-content>\n                    )\u2014and 10 controls. The results suggested that plasma Dkk\u20101 levels could differentiate\n                    <jats:styled-content style=\"fixed-case\">ET<\/jats:styled-content>\n                    from pre\u2010\n                    <jats:styled-content style=\"fixed-case\">PMF<\/jats:styled-content>\n                    , in\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">JAK<\/jats:styled-content>\n                      2\n                    <\/jats:italic>\n                    V617F\u2010positive as well as in\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">CALR<\/jats:styled-content>\n                    <\/jats:italic>\n                    \u2010positive patients, and also\n                    <jats:styled-content style=\"fixed-case\">ET<\/jats:styled-content>\n                    from\n                    <jats:styled-content style=\"fixed-case\">PV<\/jats:styled-content>\n                    in\n                    <jats:italic>\n                      <jats:styled-content style=\"fixed-case\">JAK<\/jats:styled-content>\n                      2\n                    <\/jats:italic>\n                    V617F\u2010positive patients.\n                  <\/jats:p>","DOI":"10.1111\/jcmm.13753","type":"journal-article","created":{"date-parts":[[2018,7,5]],"date-time":"2018-07-05T08:06:20Z","timestamp":1530777980000},"page":"4005-4011","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Increased Dkk\u20101 plasma levels may discriminate disease subtypes in myeloproliferative neoplasms"],"prefix":"10.1111","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8495-7704","authenticated-orcid":false,"given":"Cristina","family":"Mambet","sequence":"first","affiliation":[{"name":"MyeloAL Program Stefan S Nicolau Institute of Virology Bucharest 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Coltea Clinical Hospital Bucharest Romania"},{"name":"Carol Davila University of Medicine and Pharmacy Bucharest Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2122-0235","authenticated-orcid":false,"given":"Cristiana","family":"Tanase","sequence":"additional","affiliation":[{"name":"Biochemistry\u2010Proteomics Laboratory \u201cVictor Babes\u201d National Institute of Pathology Bucharest Romania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2259-1425","authenticated-orcid":false,"given":"Carmen C.","family":"Diaconu","sequence":"additional","affiliation":[{"name":"MyeloAL Program Stefan S Nicolau Institute of Virology Bucharest 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