{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T10:43:21Z","timestamp":1789987401345,"version":"4.0.1"},"reference-count":27,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2018,7,25]],"date-time":"2018-07-25T00:00:00Z","timestamp":1532476800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81500280"],"award-info":[{"award-number":["81500280"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2018,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>This study was aimed at investigating the effects of lnc<jats:styled-content style=\"fixed-case\">RNA AK<\/jats:styled-content>139328 on myocardial ischaemia\/reperfusion injury (<jats:styled-content style=\"fixed-case\">MIRI<\/jats:styled-content>) in diabetic mice. Ischaemia\/reperfusion (I\/R) model was constructed in normal mice (<jats:styled-content style=\"fixed-case\">NM<\/jats:styled-content>) and diabetic mice (<jats:styled-content style=\"fixed-case\">DM<\/jats:styled-content>). Microarray analysis was utilized to identify lnc<jats:styled-content style=\"fixed-case\">RNA AK<\/jats:styled-content>139328 overexpressed in <jats:styled-content style=\"fixed-case\">DM<\/jats:styled-content> after myocardial ischaemia\/reperfusion (<jats:styled-content style=\"fixed-case\">MI<\/jats:styled-content>\/R). <jats:styled-content style=\"fixed-case\">RT<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">qPCR<\/jats:styled-content> assay was utilized to investigate the expressions of lnc<jats:styled-content style=\"fixed-case\">RNA AK<\/jats:styled-content>139328 and miR\u2010204\u20103p in cardiomyocyte and tissues. Left ventricular end diastolic diameter (<jats:styled-content style=\"fixed-case\">LVEDD<\/jats:styled-content>), left ventricular end systolic diameter (<jats:styled-content style=\"fixed-case\">LVESD<\/jats:styled-content>), left ventricular ejection fraction (<jats:styled-content style=\"fixed-case\">LVEF<\/jats:styled-content>) and fractioning shortening (<jats:styled-content style=\"fixed-case\">FS<\/jats:styled-content>) were obtained by transthoracic echocardiography. Haematoxylin\u2010eosin (<jats:styled-content style=\"fixed-case\">HE<\/jats:styled-content>) staining and Masson staining were utilized to detect the damage of myocardial tissues degradation of myocardial fibres and integrity of myocardial collagen fibres. Evans Blue\/<jats:styled-content style=\"fixed-case\">TTC<\/jats:styled-content> staining was used to determine the myocardial infarct size. <jats:styled-content style=\"fixed-case\">TUNEL<\/jats:styled-content> staining was utilized to investigate cardiomyocyte apoptosis. The targeted relationship between lnc<jats:styled-content style=\"fixed-case\">RNA AK<\/jats:styled-content>139328 and miR\u2010204\u20103p was confirmed by dual\u2010luciferase reporter gene assay. <jats:styled-content style=\"fixed-case\">MTT<\/jats:styled-content> assay was used for analysis of cardiomyocyte proliferation. Western blot was utilized to investigate the expression of alpha smooth muscle actin (\u03b1\u2010<jats:styled-content style=\"fixed-case\">SMA<\/jats:styled-content>), Atg7, Atg5, <jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>3\u2010<jats:styled-content style=\"fixed-case\">II<\/jats:styled-content>\/<jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>3\u2010I and p62 marking autophagy. Knockdown of lnc<jats:styled-content style=\"fixed-case\">RNA AK<\/jats:styled-content>139328 relieved myocardial ischaemia\/reperfusion injury in <jats:styled-content style=\"fixed-case\">DM<\/jats:styled-content> and inhibited cardiomyocyte autophagy as well as apoptosis of <jats:styled-content style=\"fixed-case\">DM<\/jats:styled-content>. Lnc<jats:styled-content style=\"fixed-case\">RNA AK<\/jats:styled-content>139328 modulated miR\u2010204\u20103p directly. MiR\u2010204\u20103p and knockdown of lnc<jats:styled-content style=\"fixed-case\">RNA AK<\/jats:styled-content>139328 relieved hypoxia\/reoxygenation injury via inhibiting cardiomyocyte autophagy. Silencing lnc<jats:styled-content style=\"fixed-case\">RNA AK<\/jats:styled-content>139328 significantly increased miR\u2010204\u20103p expression and inhibited cardiomyocyte autophagy, thereby attenuating <jats:styled-content style=\"fixed-case\">MIRI<\/jats:styled-content> in <jats:styled-content style=\"fixed-case\">DM<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/jcmm.13754","type":"journal-article","created":{"date-parts":[[2018,7,26]],"date-time":"2018-07-26T04:09:41Z","timestamp":1532578181000},"page":"4886-4898","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":132,"title":["Knockdown of lnc<scp>RNA AK<\/scp>139328 alleviates myocardial ischaemia\/reperfusion injury in diabetic mice via modulating miR\u2010204\u20103p and inhibiting autophagy"],"prefix":"10.1111","volume":"22","author":[{"given":"Si\u2010yang","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Cardiovascular Medicine The Second Xiangya Hospital Central South University Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Dong","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Medicine The Second Xiangya Hospital Central South University Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhen\u2010fei","family":"Fang","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Medicine The Second Xiangya Hospital Central South University Changsha Hunan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin\u2010qun","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Medicine The Second Xiangya Hospital Central South University Changsha Hunan 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