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Many studies have highlighted the significant roles of intronic mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s and their host genes in various disease, few was mentioned in <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content> although. In this study, miR\u2010483\u20103p along with its host gene <jats:styled-content style=\"fixed-case\">IGF<\/jats:styled-content>2 (Insulin\u2010like growth factor 2) was found down\u2010regulated in 60 <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content> aganglionic colon tissues compared with 60 normal controls. <jats:styled-content style=\"fixed-case\">FHL<\/jats:styled-content>1 (Four and a half <jats:styled-content style=\"fixed-case\">LIM<\/jats:styled-content> domains 1) was determined as a target gene of miR\u2010483\u20103p via dual\u2010luciferase reporter assay, and its expression was at a higher level in <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content> tissues. Here, we study cell migration and proliferation in human 293T and <jats:styled-content style=\"fixed-case\">SH<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">SY<\/jats:styled-content>5Y cell lines by performing Transwell and <jats:styled-content style=\"fixed-case\">CCK<\/jats:styled-content>8 assays. In conclusion, the knockdown of miR\u2010483\u20103p and <jats:styled-content style=\"fixed-case\">IGF<\/jats:styled-content>2 both suppressed cell migration and proliferation, while the loss of <jats:styled-content style=\"fixed-case\">FHL<\/jats:styled-content>1 leads to opposite outcome. Furthermore, miR\u2010483\u20103p mimics could rescue the negative effects on cell proliferation and migration caused by silencing <jats:styled-content style=\"fixed-case\">IGF<\/jats:styled-content>2, while the <jats:styled-content style=\"fixed-case\">FHL<\/jats:styled-content>1 si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> may inverse the function of miR\u2010483\u20103p inhibitor. This study revealed that miR\u2010483\u20103p derived from <jats:styled-content style=\"fixed-case\">IGF<\/jats:styled-content>2 was associated with Hirschsprung's disease by targeting <jats:styled-content style=\"fixed-case\">FHL<\/jats:styled-content>1 and may provide a new pathway to understand the aetiology of <jats:styled-content style=\"fixed-case\">HSCR<\/jats:styled-content>.<\/jats:p>","DOI":"10.1111\/jcmm.13756","type":"journal-article","created":{"date-parts":[[2018,8,7]],"date-time":"2018-08-07T04:15:59Z","timestamp":1533615359000},"page":"4913-4921","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["<scp>IGF<\/scp>2\u2010derived miR\u2010483\u20103p associated with Hirschsprung's disease by targeting <scp>FHL<\/scp>1"],"prefix":"10.1111","volume":"22","author":[{"given":"Zhengke","family":"Zhi","sequence":"first","affiliation":[{"name":"Department of Pediatric Surgery Children's Hospital of Nanjing 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