{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T13:42:16Z","timestamp":1740145336032,"version":"3.37.3"},"reference-count":15,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2018,7,25]],"date-time":"2018-07-25T00:00:00Z","timestamp":1532476800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000268","name":"Biotechnology and Biological Sciences Research Council","doi-asserted-by":"publisher","award":["BB\/G015988\/1","BB\/GO15147\/1"],"award-info":[{"award-number":["BB\/G015988\/1","BB\/GO15147\/1"]}],"id":[{"id":"10.13039\/501100000268","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2018,10]]},"abstract":"Abstract<\/jats:title>Ageing is associated with impaired neuromuscular function of the terminal gastrointestinal (GI<\/jats:styled-content>) tract, which can result in chronic constipation, faecal impaction and incontinence. Interstitial cells of cajal (ICC<\/jats:styled-content>) play an important role in regulation of intestinal smooth muscle contraction. However, changes in ICC<\/jats:styled-content> volume with age in the terminal GI<\/jats:styled-content> tract (the anal canal including the anal sphincter region and rectum) have not been studied. Here, the distribution, morphology and network volume of ICC<\/jats:styled-content> in the terminal GI<\/jats:styled-content> tract of 3\u2010 to 4\u2010month\u2010old and 26\u2010 to 28\u2010month\u2010old C57BL<\/jats:styled-content>\/6 mice were investigated. ICC<\/jats:styled-content> were identified by immunofluorescence labelling of wholemount preparations with an antibody against c\u2010Kit. ICC<\/jats:styled-content> network volume was measured by software\u2010based 3D volume rendering of confocal Z stacks. A significant reduction in ICC<\/jats:styled-content> network volume per unit volume of muscle was measured in aged animals. No age\u2010associated change in ICC<\/jats:styled-content> morphology was detected. The thickness of the circular muscle layer of the anal sphincter region and rectum increased with age, while that in the distal colon decreased. These results suggest that ageing is associated with a reduction in the network volume of ICC<\/jats:styled-content> in the terminal GI<\/jats:styled-content> tract, which may influence the normal function of these regions.<\/jats:p>","DOI":"10.1111\/jcmm.13794","type":"journal-article","created":{"date-parts":[[2018,7,26]],"date-time":"2018-07-26T04:09:11Z","timestamp":1532578151000},"page":"5160-5164","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Interstitial cell network volume is reduced in the terminal bowel of ageing mice"],"prefix":"10.1111","volume":"22","author":[{"given":"Prasanna P. K. M.","family":"Gamage","sequence":"first","affiliation":[{"name":"School of Life, Health and Chemical Sciences Open University Milton Keynes UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bhavik A.","family":"Patel","sequence":"additional","affiliation":[{"name":"School of Pharmacy and Biomolecular Sciences University of Brighton Brighton UK"},{"name":"Centre for Stress and Age\u2010Related Disease University of Brighton Brighton UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mark S.","family":"Yeoman","sequence":"additional","affiliation":[{"name":"School of Pharmacy and Biomolecular Sciences University of Brighton Brighton UK"},{"name":"Centre for Stress and Age\u2010Related Disease University of Brighton Brighton UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rachel N.","family":"Ranson","sequence":"additional","affiliation":[{"name":"Department of Applied Sciences Faculty of Health and Life Sciences Northumbria University Newcastle upon Tyne UK"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6786-2922","authenticated-orcid":false,"given":"M. Jill","family":"Saffrey","sequence":"additional","affiliation":[{"name":"School of Life, Health and Chemical Sciences Open University Milton Keynes UK"},{"name":"Department of Applied Sciences Faculty of Health and Life Sciences Northumbria University Newcastle upon Tyne UK"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,7,25]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cger.2013.10.003"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11357-013-9603-2"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.exger.2014.02.007"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep42754"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1152\/physiol.00006.2016"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-981-10-1061-3_7"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2010.01590.x"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00304.2012"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0169113"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.5056\/jnm17061"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00196.2011"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2014.272542"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00331.2014"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-011-1138-1"},{"key":"e_1_2_7_16_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2982.2008.01109.x"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjcmm.13794","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.13794","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/jcmm.13794","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.13794","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,2]],"date-time":"2023-09-02T06:57:59Z","timestamp":1693637879000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.13794"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,7,25]]},"references-count":15,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2018,10]]}},"alternative-id":["10.1111\/jcmm.13794"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.13794","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"type":"print","value":"1582-1838"},{"type":"electronic","value":"1582-4934"}],"subject":[],"published":{"date-parts":[[2018,7,25]]},"assertion":[{"value":"2018-05-17","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-05-18","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-07-25","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}