{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T17:28:39Z","timestamp":1790270919715,"version":"4.1.0"},"reference-count":58,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2018,9,6]],"date-time":"2018-09-06T00:00:00Z","timestamp":1536192000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81600220"],"award-info":[{"award-number":["81600220"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100007129","name":"Natural Science Foundation of Shandong Province","doi-asserted-by":"publisher","award":["BS2014YY020"],"award-info":[{"award-number":["BS2014YY020"]}],"id":[{"id":"10.13039\/501100007129","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Podocyte injury is an early pathological change characteristic of various glomerular diseases, and apoptosis and F\u2010actin cytoskeletal disruption are typical features of podocyte injury. In this study, we found that adriamycin (<jats:styled-content style=\"fixed-case\">ADR<\/jats:styled-content>) treatment resulted in typical podocyte injury and repressed plectin expression. Restoring plectin expression protected against <jats:styled-content style=\"fixed-case\">ADR<\/jats:styled-content>\u2010induced podocyte injury whereas si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>\u2010mediated plectin silencing produced similar effects as <jats:styled-content style=\"fixed-case\">ADR<\/jats:styled-content>\u2010induced podocyte injury, suggesting that plectin plays a key role in preventing podocyte injury. Further analysis showed that plectin repression induced significant integrin \u03b16\u03b24, focal adhesion kinase (<jats:styled-content style=\"fixed-case\">FAK<\/jats:styled-content>) and p38 <jats:styled-content style=\"fixed-case\">MAPK<\/jats:styled-content> phosphorylation. Mutating Y1494, a key tyrosine residue in the integrin \u03b24 subunit, blocked <jats:styled-content style=\"fixed-case\">FAK<\/jats:styled-content> and p38 phosphorylation, thereby alleviating podocyte injury. Inhibitor studies demonstrated that <jats:styled-content style=\"fixed-case\">FAK<\/jats:styled-content> Y397 phosphorylation promoted p38 activation, resulting in podocyte apoptosis and F\u2010actin cytoskeletal disruption. In vivo studies showed that administration of <jats:styled-content style=\"fixed-case\">ADR<\/jats:styled-content> to rats resulted in significantly increased 24\u2010hour urine protein levels along with decreased plectin expression and activated integrin \u03b16\u03b24, <jats:styled-content style=\"fixed-case\">FAK<\/jats:styled-content>, and p38. Taken together, these findings indicated that plectin protects podocytes from <jats:styled-content style=\"fixed-case\">ADR<\/jats:styled-content>\u2010induced apoptosis and F\u2010actin cytoskeletal disruption by inhibiting integrin \u03b16\u03b24\/<jats:styled-content style=\"fixed-case\">FAK<\/jats:styled-content>\/p38 pathway activation and that plectin may be a therapeutic target for podocyte injury\u2010related glomerular diseases.<\/jats:p>","DOI":"10.1111\/jcmm.13816","type":"journal-article","created":{"date-parts":[[2018,9,6]],"date-time":"2018-09-06T11:50:22Z","timestamp":1536234622000},"page":"5450-5467","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":41,"title":["Plectin protects podocytes from adriamycin\u2010induced apoptosis and F\u2010actin cytoskeletal disruption through the integrin \u03b16\u03b24\/<scp>FAK<\/scp>\/p38 <scp>MAPK<\/scp> pathway"],"prefix":"10.1111","volume":"22","author":[{"given":"Yongliang","family":"Ni","sequence":"first","affiliation":[{"name":"Department of Geriatrics Qilu Hospital of Shandong University Jinan Shandong China"},{"name":"Department of Urology Shandong Provincial Third Hospital Jinan Shandong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Urology Tengzhou Central People's Hospital affiliated to Jining Medical College Xintan Road 181 Tengzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoxuan","family":"Yin","sequence":"additional","affiliation":[{"name":"Department of Traditional Chinese Medicine Yankuang Group General Hospital Zoucheng China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Urology Qilu Hospital of Shandong University Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xigao","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Urology Qilu Hospital of Shandong University Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haixin","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Urology Yankuang Group General Hospital Zoucheng China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangjv","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Geriatrics Qilu Hospital of Shandong University Jinan Shandong China"},{"name":"Key Laboratory of Cardiovascular Proteomics of Shandong Province Qilu Hospital of Shandong University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jun","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Geriatrics Qilu Hospital of Shandong University Jinan Shandong China"},{"name":"Key Laboratory of Cardiovascular Proteomics of Shandong Province Qilu Hospital of Shandong University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haiqing","family":"Gao","sequence":"additional","affiliation":[{"name":"Department of Geriatrics Qilu Hospital of Shandong University Jinan Shandong China"},{"name":"Key Laboratory of Cardiovascular Proteomics of Shandong Province Qilu Hospital of Shandong University"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benkang","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Urology Qilu Hospital of Shandong University Jinan China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shaohua","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Geriatrics Qilu Hospital of Shandong University Jinan Shandong China"},{"name":"Key Laboratory of Cardiovascular Proteomics of Shandong Province Qilu Hospital of Shandong University"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,9,6]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.2337\/db15-0473"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2014090916"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrneph.2015.79"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.13001"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3843"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2015.05.008"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/jid.2013.498"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2009.48"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.str.2015.01.011"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1096\/fj.13-231829"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/s12935-015-0177-1"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.tcb.2016.02.001"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb3333"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2009090991"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00380-011-0219-0"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1097\/HJH.0b013e32835d6a56"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-med-050311-163340"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1080\/09687680802269886"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI71195"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI43721"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2015.478"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1349-7006.2009.01282.x"},{"key":"e_1_2_8_24_1","first-page":"549","article-title":"Cell pleomorphism and cytoskeleton disorganization in human liver cancer","volume":"30","author":"Cheng CC","year":"2016","journal-title":"In Vivo"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jprot.2011.12.018"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2010.12.008"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1158\/0008-5472.CAN-08-2125"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-013-0860-4"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2016.335"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2004121084"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI58552"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI79504"},{"key":"e_1_2_8_33_1","first-page":"319","article-title":"Perinuclear distribution of plectin characterizes visceral epithelial cells of rat glomeruli","volume":"149","author":"Yaoita E","year":"1996","journal-title":"Am J Pathol"},{"issue":"3","key":"e_1_2_8_34_1","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1242\/jcs.113.3.483","article-title":"Dose\u2010dependent linkage, assembly inhibition and disassembly of vimentin and cytokeratin 5\/14 filaments through plectin's intermediate filament\u2010binding domain","volume":"113","author":"Steinbock FA","year":"2000","journal-title":"J Cell Sci"},{"key":"e_1_2_8_35_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI71919"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm.1857"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2016070709"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/jb\/mvv020"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11010-014-2220-2"},{"key":"e_1_2_8_40_1","first-page":"1059","article-title":"[Transforming growth factor\u2010beta1 regulates renal alpha3 and beta1 integrin expressions in diabetic rats: a new insight into the renoprotective effect of irbesartan]","volume":"31","author":"Zhu Y","year":"2011","journal-title":"Nan Fang Yi Ke Da Xue Xue Bao"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI57673"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2014.380"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.2015.82"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.141.1.209"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0969-2126(03)00090-X"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.113.6.963"},{"key":"e_1_2_8_47_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M411818200"},{"key":"e_1_2_8_48_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109.008474"},{"key":"e_1_2_8_49_1","doi-asserted-by":"publisher","DOI":"10.1158\/1541-7786.MCR-09-0102"},{"key":"e_1_2_8_50_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.00326-10"},{"key":"e_1_2_8_51_1","doi-asserted-by":"publisher","DOI":"10.18632\/oncotarget.7646"},{"key":"e_1_2_8_52_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.E11-11-0957"},{"key":"e_1_2_8_53_1","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/135608"},{"key":"e_1_2_8_54_1","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2015.300"},{"key":"e_1_2_8_55_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2011.03.048"},{"key":"e_1_2_8_56_1","doi-asserted-by":"crossref","first-page":"51955","DOI":"10.18632\/oncotarget.10192","article-title":"ST6Gal\u2010I modulates docetaxel sensitivity in human hepatocarcinoma cells via the p38 MAPK\/caspase pathway","volume":"7","author":"Chen X","year":"2016","journal-title":"Oncotarget"},{"key":"e_1_2_8_57_1","doi-asserted-by":"publisher","DOI":"10.1038\/ncb1400"},{"key":"e_1_2_8_58_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI71165"},{"key":"e_1_2_8_59_1","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2016040414"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjcmm.13816","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.13816","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,14]],"date-time":"2023-09-14T17:07:15Z","timestamp":1694711235000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.13816"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,9,6]]},"references-count":58,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2018,11]]}},"alternative-id":["10.1111\/jcmm.13816"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.13816","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,9,6]]},"assertion":[{"value":"2017-06-20","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-06-29","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-09-06","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}