{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,18]],"date-time":"2026-01-18T12:12:22Z","timestamp":1768738342362,"version":"3.49.0"},"reference-count":24,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2018,9,4]],"date-time":"2018-09-04T00:00:00Z","timestamp":1536019200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>The treatment of complicated long segment strictures remains to a challenge, and the substitution urethroplasty treatment is often accompanied by subsequent tissue fibrosis and secondary stricture formation. In situ injection of human adipose tissue\u2010derived stem cells (hADSC) could potential be applied for prevention of urethral fibrosis, but the cells transplantation alone may be insufficient because of the complicated histopathological micro\u2010environmental changes in the injury site. This study investigated whether miR\u201021 modification can improve the therapeutic efficacy of ADSCs against urethral fibrosis to limit urethral stricture recurrence. MiR\u201021\u2010modified ADSCs (miR\u201021) were constructed via lentivirus\u2010mediated transfer of pre\u2010miR\u201021 and GFP reporter gene. In vitro results suggested that miR\u201021 modification can increase the angiogenesis genes expression of ADSCs and enhance its anti\u2010oxidative effects against reactive oxygen species (ROS) damage. In vivo results showed that miR\u201021 modification contributes to increased urodynamic parameters and better formation of the epithelium and the muscle layer as compared to ADSCs transplantation alone groups. The results demonstrated that miR\u201021 modification in ADSCs could improve urethral wound healing microenvironment, enhance stem cell survival through ROS scavenging and promote the neovascularization via regulating angiogenic genes expression, which eventually increase the ADSCs' therapeutic potential for urethral wound healing.<\/jats:p>","DOI":"10.1111\/jcmm.13834","type":"journal-article","created":{"date-parts":[[2018,9,4]],"date-time":"2018-09-04T14:08:13Z","timestamp":1536070093000},"page":"5607-5616","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["miR\u201021 modification enhances the performance of adipose tissue\u2010derived mesenchymal stem cells for counteracting urethral stricture 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