{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,16]],"date-time":"2026-07-16T12:19:50Z","timestamp":1784204390130,"version":"3.55.0"},"reference-count":50,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2018,9,6]],"date-time":"2018-09-06T00:00:00Z","timestamp":1536192000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Shire Innovation Fund"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Intestinal mesenchymal cells deposit extracellular matrix in fibrotic Crohn's disease (CD). The contribution of epithelial to mesenchymal transition (EMT) to the mesenchymal cell pool in CD fibrosis remains obscure. The miR\u2010200 family regulates fibrosis\u2010related EMT in organs other than the gut. E\u2010cadherin, cytokeratin\u201018 and vimentin expression was assessed using immunohistochemistry on paired strictured (SCD) and non\u2010strictured (NSCD) ileal CD resections and correlated with fibrosis grade. MiR\u2010200 expression was measured in paired SCD and NSCD tissue compartments using laser capture microdissection and RT\u2010qPCR. Serum miR\u2010200 expression was also measured in healthy controls and CD patients with stricturing and non\u2010stricturing phenotypes. Extra\u2010epithelial cytokeratin\u201018 staining and vimentin\u2010positive epithelial staining were significantly greater in SCD samples (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.04 and <jats:italic>P<\/jats:italic>\u00a0=\u00a00.03, respectively). Cytokeratin\u201018 staining correlated positively with subserosal fibrosis (<jats:italic>P<\/jats:italic>\u00a0&lt;\u00a00.001). Four miR\u2010200 family members were down\u2010regulated in fresh SCD samples (miR\u2010141, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.002; miR\u2010200a, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.002; miR\u2010200c, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.001; miR\u2010429; <jats:italic>P<\/jats:italic>\u00a0=\u00a00.004); miR\u2010200 down\u2010regulation in SCD tissue was localised to the epithelium (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.001\u20100.015). The miR\u2010200 target <jats:italic>ZEB1<\/jats:italic> was up\u2010regulated in SCD samples (<jats:italic>P<\/jats:italic>\u00a0=\u00a00.035). No difference in serum expression between patient groups was observed. Together, these observations suggest the presence of EMT in CD strictures and implicate the miR\u2010200 family as regulators. Functional studies to prove this relationship are now warranted.<\/jats:p>","DOI":"10.1111\/jcmm.13836","type":"journal-article","created":{"date-parts":[[2018,9,6]],"date-time":"2018-09-06T12:00:35Z","timestamp":1536235235000},"page":"5617-5628","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":32,"title":["Epithelial down\u2010regulation of the miR\u2010200 family in fibrostenosing Crohn's disease is associated with features of epithelial to mesenchymal transition"],"prefix":"10.1111","volume":"22","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7002-293X","authenticated-orcid":false,"given":"Shameer J.","family":"Mehta","sequence":"first","affiliation":[{"name":"Centre for Genomics and Child Health Blizard Institute Barts and The London School of Medicine &amp; Dentistry London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy","family":"Lewis","sequence":"additional","affiliation":[{"name":"Centre 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