{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,13]],"date-time":"2026-01-13T16:04:28Z","timestamp":1768320268558,"version":"3.49.0"},"reference-count":26,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2018,9,14]],"date-time":"2018-09-14T00:00:00Z","timestamp":1536883200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010883","name":"Traditional Chinese Medicine Bureau of Guangdong Province","doi-asserted-by":"publisher","award":["20172072"],"award-info":[{"award-number":["20172072"]}],"id":[{"id":"10.13039\/501100010883","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010618","name":"Guangzhou University of Chinese Medicine","doi-asserted-by":"publisher","award":["YN2015MS21"],"award-info":[{"award-number":["YN2015MS21"]}],"id":[{"id":"10.13039\/501100010618","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2018,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>micro<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s (mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s) post\u2010transcriptionally regulate cardiac repair following myocardial infarction (<jats:styled-content style=\"fixed-case\">MI<\/jats:styled-content>). Omega\u20103 polyunsaturated fatty acid (\u03c9\u20103 <jats:styled-content style=\"fixed-case\">PUFA<\/jats:styled-content>s) may support cardiac healing after <jats:styled-content style=\"fixed-case\">MI<\/jats:styled-content>, but the mechanism is unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The <jats:italic>fat\u20101<\/jats:italic> transgenic mouse expresses a \u03c9\u20103 fatty acid desaturase which converts \u03c9\u20106 <jats:styled-content style=\"fixed-case\">PUFA<\/jats:styled-content>s to \u03c9\u20103 <jats:styled-content style=\"fixed-case\">PUFA<\/jats:styled-content>s in\u00a0vivo. <jats:styled-content style=\"fixed-case\">MI<\/jats:styled-content> was induced in <jats:italic>fat\u20101<\/jats:italic> transgenic (n\u00a0=\u00a030) and wild\u2010type (<jats:styled-content style=\"fixed-case\">WT<\/jats:styled-content>) mice (n\u00a0=\u00a030) using permanent ligation. Other transgenic and <jats:styled-content style=\"fixed-case\">WT<\/jats:styled-content> mice underwent sham procedure (n\u00a0=\u00a030 and n\u00a0=\u00a030, respectively). One week after occlusion, cardiac function was measured by echocardiography and the infarct size was assessed using histology and mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> microarray profiling. Expression of selected mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> was confirmed using quantitative real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>One week following <jats:styled-content style=\"fixed-case\">MI<\/jats:styled-content>, the <jats:italic>fat\u20101<\/jats:italic> transgenic myocardium had better cardiac function, a smaller fibrotic area, and fewer apoptotic cardiomyocytes than <jats:styled-content style=\"fixed-case\">WT<\/jats:styled-content> myocardium. Post\u2010<jats:styled-content style=\"fixed-case\">MI<\/jats:styled-content> profiling showed 33 mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s that were significantly up\u2010regulated, and 35 were down\u2010regulated, in <jats:italic>fat\u20101<\/jats:italic> group compared to the <jats:styled-content style=\"fixed-case\">WT<\/jats:styled-content> group (n\u00a0=\u00a03 and n\u00a0=\u00a02 mice, respectively). Among selected apoptosis\u2010associated mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s, 9 mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s were up\u2010regulated (miR\u2010101a\u20103p, miR\u2010128\u20103p,miR\u2010133a\u20105p,miR\u2010149\u20105p,miR\u2010192\u20105p,miR\u20101a\u20103p,miR\u2010208a\u20103p,miR\u201029c\u20105p,miR\u201030c\u20102\u20103p), and 3 were down\u2010regulated (miR\u2010210\u20103p,miR\u201021a\u20103p,miR\u2010214\u20103p) in <jats:italic>fat\u20101<\/jats:italic> transgenic mice compared with <jats:styled-content style=\"fixed-case\">WT<\/jats:styled-content> mice. Kyoto encyclopaedia of genes and genomes (<jats:styled-content style=\"fixed-case\">KEGG<\/jats:styled-content>) pathway analysis indicated likely roles for these mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s in <jats:styled-content style=\"fixed-case\">MI<\/jats:styled-content>. Furthermore, Bcl\u20102 expression was increased, and caspase\u20103 decreased, in infarcted <jats:italic>fat\u20101<\/jats:italic> transgenic mouse hearts compared to <jats:styled-content style=\"fixed-case\">WT<\/jats:styled-content> hearts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>\u03c9\u20103 <jats:styled-content style=\"fixed-case\">PUFA<\/jats:styled-content>s may have a protective effect on cardiomyocytes following <jats:styled-content style=\"fixed-case\">MI<\/jats:styled-content> through their modulation of apoptosis\u2010related mi<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s and target genes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/jcmm.13846","type":"journal-article","created":{"date-parts":[[2018,9,16]],"date-time":"2018-09-16T05:37:08Z","timestamp":1537076228000},"page":"5698-5707","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Modulation of 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