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Selenocysteine (Sec) Insertion Sequence (<jats:styled-content style=\"fixed-case\">SECIS<\/jats:styled-content>) and <jats:styled-content style=\"fixed-case\">SECIS<\/jats:styled-content> binding protein 2 (<jats:styled-content style=\"fixed-case\">SBP<\/jats:styled-content>2) interaction is a very critical node for the metabolic balance between Se and selenoproteins. The <jats:italic>Gpx1<\/jats:italic>,<jats:italic> Gpx4<\/jats:italic> and <jats:italic>SelS<\/jats:italic> have different binding affinities with <jats:styled-content style=\"fixed-case\">SBP<\/jats:styled-content>2 in cells. According to our results, both <jats:italic>miR\u2010181a\u20105p<\/jats:italic> and <jats:styled-content style=\"fixed-case\">SBP<\/jats:styled-content>2 appeared to be selenium\u2010sensitive and regulated the expression of selenoproteins in C28\/I2 cells under Se sufficient environment. However, they showed significantly opposite expression trend in Se deficiency rats cartilage and SeD C28\/I2 cells. The <jats:italic><jats:styled-content style=\"fixed-case\">SBP<\/jats:styled-content>2<\/jats:italic> is a direct target gene of <jats:italic>miR\u2010181a\u20105p<\/jats:italic> in C28\/I2 cells as determined by reporter gene and off\u2010target experiments. And the <jats:italic>miR\u2010181a\u20105p<\/jats:italic> could regulate <jats:styled-content style=\"fixed-case\">SBP<\/jats:styled-content>2 and the selenoproteins in C28\/I2 cells. Depending upon the Se supply levels, C28\/I2 cells were divided into three groups, that is normal Se, SeD and SeS, which underwent through a 7\u2010day Se deprivation process, then <jats:italic><jats:styled-content style=\"fixed-case\">SBP<\/jats:styled-content>2<\/jats:italic> was knocked\u2010down and overexpressed in all the groups. Moreover, the selected selenoproteins were down\u2010regulated in second\u2010generation low Se diet rat cartilage. The selenoproteins expression was decreased by Se deficiency which depended on the Selenium\u2010sensitive <jats:italic>miR\u2010181a\u20105p<\/jats:italic> to participate and regulate <jats:styled-content style=\"fixed-case\">SBP<\/jats:styled-content>2 at post\u2010transcriptional level. It involves a series of antioxidant and <jats:styled-content style=\"fixed-case\">ECM<\/jats:styled-content> (extracellular matrix) genes, to overcome the <jats:styled-content style=\"fixed-case\">ROS<\/jats:styled-content>\u2010related stress for the protection of essential physiological functions and to maintain the balance between anabolism and catabolism of the cartilage.<\/jats:p>","DOI":"10.1111\/jcmm.13858","type":"journal-article","created":{"date-parts":[[2018,9,24]],"date-time":"2018-09-24T12:37:26Z","timestamp":1537792646000},"page":"5888-5898","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Selenium\u2010sensitive <i>mi<scp>RNA<\/scp>\u2010181a\u20105p<\/i> targeting <i><scp>SBP<\/scp>2<\/i> regulates selenoproteins expression in cartilage"],"prefix":"10.1111","volume":"22","author":[{"given":"Zixin","family":"Min","sequence":"first","affiliation":[{"name":"Department of Biochemistry and Molecular 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