{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,24]],"date-time":"2026-08-24T19:44:32Z","timestamp":1787600672097,"version":"build-2736575974"},"reference-count":29,"publisher":"Wiley","issue":"12","license":[{"start":{"date-parts":[[2018,10,19]],"date-time":"2018-10-19T00:00:00Z","timestamp":1539907200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Sciences Foundation of China","doi-asserted-by":"crossref","award":["81202352"],"award-info":[{"award-number":["81202352"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Sciences Foundation of China","doi-asserted-by":"crossref","award":["81171504"],"award-info":[{"award-number":["81171504"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Oxidative stress leads to melanocyte death and has been implicated in the pathogenesis of vitiligo. The nuclear factor, E2\u2010related factor 2 (Nrf2), is a critical transcription factor in protecting cells from oxidative damage. High\u2010mobility group box 1 (<jats:styled-content style=\"fixed-case\">HMGB<\/jats:styled-content>1) is a chromatin\u2010associated nuclear protein and an extracellular damage\u2010associated molecular pattern molecule. Extracellular <jats:styled-content style=\"fixed-case\">HMGB<\/jats:styled-content>1 released from activated immune cells, necrotic or injured cells, becomes a proinflammatory mediator through binding to cell\u2010surface receptors of responding cells. In this study, we investigated the role of <jats:styled-content style=\"fixed-case\">HMGB<\/jats:styled-content>1 from melanocytes in the response to oxidative stress and the mechanism involved. We showed that <jats:styled-content style=\"fixed-case\">HMGB<\/jats:styled-content>1 is expressed by primary normal human epidermal melanocytes (<jats:styled-content style=\"fixed-case\">NHEM<\/jats:styled-content>s). H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub> treatment increased cytoplasmic translocation and extracellular release of <jats:styled-content style=\"fixed-case\">HMGB<\/jats:styled-content>1. <jats:styled-content style=\"fixed-case\">HMGB<\/jats:styled-content>1 knockdown by small interfering <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> (si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>) led to decreased apoptosis of <jats:styled-content style=\"fixed-case\">NHEM<\/jats:styled-content>s. <jats:styled-content style=\"fixed-case\">HMGB<\/jats:styled-content>1 inhibition enhanced the expression of Nrf2 and its target genes. The expression of Nrf2 and its downstream antioxidant genes was downregulated after the supernatant of H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>\u2010treated <jats:styled-content style=\"fixed-case\">NHEM<\/jats:styled-content>s was added to <jats:styled-content style=\"fixed-case\">HMGB<\/jats:styled-content>1\u2010deficient cells. <jats:styled-content style=\"fixed-case\">HMGB<\/jats:styled-content>1 knockdown by si<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> suppressed the expression of the autophagosome marker, <jats:styled-content style=\"fixed-case\">LC<\/jats:styled-content>3, and enhanced p62 expression. Coimmunoprecipitation with Keap1 showed a reduced Nrf2\u2010Keap1 interaction and an increased p62\u2010Keap1 interaction under oxidative stress. These data demonstrated that external stimuli (eg, oxidative stress) may trigger autocrine <jats:styled-content style=\"fixed-case\">HMGB<\/jats:styled-content>1 translocation and release by melanocytes, suppressing the expression of Nrf2 and downstream antioxidant genes to induce melanocyte apoptosis, and thereby participate in the pathological process of vitiligo.<\/jats:p>","DOI":"10.1111\/jcmm.13895","type":"journal-article","created":{"date-parts":[[2018,10,19]],"date-time":"2018-10-19T09:30:51Z","timestamp":1539941451000},"page":"6148-6156","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":38,"title":["<scp>HMGB<\/scp>1 deficiency reduces H<sub>2<\/sub>O<sub>2<\/sub>\u2010induced oxidative damage in human melanocytes via the Nrf2 pathway"],"prefix":"10.1111","volume":"22","author":[{"given":"Kuanhou","family":"Mou","sequence":"first","affiliation":[{"name":"Department of Dermatology The First Affiliated Hospital of Xi'an Jiaotong University Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Dermatology The First Affiliated Hospital of Xi'an Jiaotong University Xi'an Shaanxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Miao","sequence":"additional","affiliation":[{"name":"Center for Translational Medicine The First Affiliated Hospital of Xi'an Jiaotong University Xi'an Shaanxi China"},{"name":"Key Laboratory for Tumor Precision Medicine of Shaanxi Province The First Affiliated Hospital of Xi'an Jiaotong University Xi'an China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fang","family":"Cao","sequence":"additional","affiliation":[{"name":"Center for Translational Medicine The First Affiliated Hospital of Xi'an Jiaotong University Xi'an Shaanxi China"},{"name":"Key Laboratory for Tumor Precision Medicine of Shaanxi Province The 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