{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,25]],"date-time":"2026-08-25T07:06:07Z","timestamp":1787641567595,"version":"build-2736575974"},"reference-count":45,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,11,5]],"date-time":"2018-11-05T00:00:00Z","timestamp":1541376000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81070633"],"award-info":[{"award-number":["81070633"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81360060"],"award-info":[{"award-number":["81360060"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["31660323"],"award-info":[{"award-number":["31660323"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["30860111"],"award-info":[{"award-number":["30860111"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100010857","name":"Jiangxi Provincial Department of Science and Technology","doi-asserted-by":"publisher","award":["20123BCB22005"],"award-info":[{"award-number":["20123BCB22005"]}],"id":[{"id":"10.13039\/501100010857","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2019,1]]},"abstract":"Abstract<\/jats:title>Previous study has shown that thiazolidinediones (TZD<\/jats:styled-content>s) improved endothelium insulin resistance (IR<\/jats:styled-content>) induced by high glucose concentration (HG<\/jats:styled-content>)\/hyperglycaemia through aPPAR<\/jats:styled-content>\u03b3\u2010dependent\u2010NF<\/jats:styled-content>\u03baB trans\u2010repression mechanism. However, it is unclear, whether changes inPPAR<\/jats:styled-content>\u03b3 expression affect the endotheliumIR<\/jats:styled-content>and what the underlying mechanism is. In the present study, we aimed to address this issue.HG<\/jats:styled-content>\u2010treated human umbilical vascular endothelial cells (HUVEC<\/jats:styled-content>) were transfected by eitherPPAR<\/jats:styled-content>\u03b3\u2010overexpressing (Ad\u2010PPAR<\/jats:styled-content>\u03b3) orPPAR<\/jats:styled-content>\u03b3\u2010shRNA<\/jats:styled-content>\u2010containing (Ad\u2010PPAR<\/jats:styled-content>\u03b3\u2010shRNA<\/jats:styled-content>) adenoviral vectors. Likewise, the rats fed by high\u2010fat diet (HFD<\/jats:styled-content>) were infected by intravenous administration of Ad\u2010PPAR<\/jats:styled-content>\u03b3 or Ad\u2010PPAR<\/jats:styled-content>\u03b3\u2010shRNA<\/jats:styled-content>. The levels of nitric oxide (NO<\/jats:styled-content>), endothelin\u20101 (ET<\/jats:styled-content>\u20101) and cytokines (TNF<\/jats:styled-content>\u03b1,IL<\/jats:styled-content>\u20106,sICAM<\/jats:styled-content>\u20101 andsVCAM<\/jats:styled-content>\u20101) and the expression levels ofPPAR<\/jats:styled-content>\u03b3,eNOS<\/jats:styled-content>,AKT<\/jats:styled-content>, p\u2010AKT<\/jats:styled-content>,IKK<\/jats:styled-content>\u03b1\/\u03b2 and p\u2010IKK<\/jats:styled-content>\u03b1\/\u03b2 and I\u03baB\u03b1 were examined; and the interaction betweenPPAR<\/jats:styled-content>\u03b3 andNF<\/jats:styled-content>\u03baB\u2010P65 as well as vascular function were evaluated. Our present results showed that overexpression ofPPAR<\/jats:styled-content>\u03b3 notably increased the levels ofNO<\/jats:styled-content>,eNOS<\/jats:styled-content>, p\u2010AKT<\/jats:styled-content>and I\u03baB\u03b1 as well as the interaction ofPPAR<\/jats:styled-content>\u03b3 andNF<\/jats:styled-content>\u03baB\u2010P65, and decreased the levels ofET<\/jats:styled-content>\u20101, p\u2010IKK<\/jats:styled-content>\u03b1\/\u03b2,TNF<\/jats:styled-content>\u03b1,IL<\/jats:styled-content>\u20106,sICAM<\/jats:styled-content>\u20101 andsVCAM<\/jats:styled-content>\u20101. In contrast, down\u2010expression ofPPAR<\/jats:styled-content>\u03b3 displayed the opposite effects. The results demonstrate that the overexpression ofPPAR<\/jats:styled-content>\u03b3 improves while the down\u2010expression worsens the endotheliumIR<\/jats:styled-content>via aPPAR<\/jats:styled-content>\u03b3\u2010mediatedNF<\/jats:styled-content>\u03baB trans\u2010repression dependent manner. The findings suggestPPAR<\/jats:styled-content>\u03b3 is a potential therapeutic target for diabetic vascular complications.<\/jats:p>","DOI":"10.1111\/jcmm.13913","type":"journal-article","created":{"date-parts":[[2018,11,6]],"date-time":"2018-11-06T06:25:44Z","timestamp":1541485544000},"page":"216-226","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Trans\u2010repression ofNF<\/scp>\u03baB pathway mediated byPPAR<\/scp>\u03b3 improves vascular endothelium insulin resistance"],"prefix":"10.1111","volume":"23","author":[{"given":"Ying","family":"Kong","sequence":"first","affiliation":[{"name":"Key Provincial Laboratory of Basic Pharmacology Nanchang University Nanchang Jiangxi China"},{"name":"Department of Pharmacology School of Pharmacy Nanchang University Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Gao","sequence":"additional","affiliation":[{"name":"Key Provincial Laboratory of Basic Pharmacology Nanchang University Nanchang Jiangxi China"},{"name":"Department of Pharmacology School of Pharmacy Nanchang University Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongyi","family":"Lan","sequence":"additional","affiliation":[{"name":"Jiangxi Medical College Nanchang University Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Zhang","sequence":"additional","affiliation":[{"name":"Key Provincial Laboratory of Basic Pharmacology Nanchang University Nanchang Jiangxi China"},{"name":"Department of Pharmacology School of Pharmacy Nanchang University Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rixin","family":"Zhan","sequence":"additional","affiliation":[{"name":"Key Provincial Laboratory of Basic Pharmacology Nanchang University Nanchang Jiangxi China"},{"name":"Department of Pharmacology School of Pharmacy Nanchang University Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meiqi","family":"Liu","sequence":"additional","affiliation":[{"name":"Key Provincial Laboratory of Basic Pharmacology Nanchang University Nanchang Jiangxi China"},{"name":"Department of Pharmacology School of Pharmacy Nanchang University Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhouan","family":"Zhu","sequence":"additional","affiliation":[{"name":"Jiangxi Medical College Nanchang University Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guohua","family":"Zeng","sequence":"additional","affiliation":[{"name":"Key Provincial Laboratory of Basic Pharmacology Nanchang University Nanchang Jiangxi China"},{"name":"Department of Pharmacology School of Pharmacy Nanchang University Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3434-9484","authenticated-orcid":false,"given":"Qiren","family":"Huang","sequence":"additional","affiliation":[{"name":"Key Provincial Laboratory of Basic Pharmacology Nanchang University Nanchang Jiangxi China"},{"name":"Department of Pharmacology School of Pharmacy Nanchang University Nanchang Jiangxi China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,11,5]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1097\/HJH.0000000000000634"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.abb.2014.02.005"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2014.274175"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1113\/jphysiol.2012.234856"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(05)67402-8"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature05488"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/nyas.12395"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1210\/jc.2014-2704"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.3945\/an.115.011403"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2016.02.028"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1517\/17460441.2015.1100164"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2015.9536"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1517\/14656566.2015.1009894"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjmed.2007.07.004"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9150(99)00085-4"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehv239"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.pharmthera.2016.09.007"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2013.05.017"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2015.02.004"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10495-010-0477-9"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2015.02.153"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2014.01.010"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2017.07.037"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1113\/expphysiol.2007.039172"},{"key":"e_1_2_8_26_1","first-page":"1069718","article-title":"Targeting peroxisome proliferator\u2010activated receptors using\u00a0thiazolidinediones: strategy for design of novel antidiabetic drugs","volume":"2017","author":"Thangavel N","year":"2017","journal-title":"Int J Med Chem"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1155\/2017\/8360919"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1155\/2016\/7614270"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1080\/14779072.2016.1207527"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2017.07.007"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00092.2005"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1038\/ajh.2011.114"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1097\/HJH.0000000000000965"},{"key":"e_1_2_8_34_1","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.116.306531"},{"key":"e_1_2_8_35_1","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1515\/hmbci-2014-0001","article-title":"Roles of oxidative stress, adiponectin, and nuclear hormone receptors in obesity\u2010associated\u00a0insulin resistance\u00a0and cardiovascular risk","volume":"19","author":"Matsuda M","year":"2014","journal-title":"Horm Mol Biol Clin Investig"},{"key":"e_1_2_8_36_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.atherosclerosis.2013.12.023"},{"key":"e_1_2_8_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellsig.2015.09.009"},{"key":"e_1_2_8_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2015.05.015"},{"key":"e_1_2_8_39_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00125-014-3438-y"},{"key":"e_1_2_8_40_1","doi-asserted-by":"publisher","DOI":"10.1038\/nature13887"},{"key":"e_1_2_8_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2015.11.014"},{"key":"e_1_2_8_42_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.phrs.2015.12.010"},{"key":"e_1_2_8_43_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbagen.2014.09.027"},{"key":"e_1_2_8_44_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10753-015-0193-2"},{"key":"e_1_2_8_45_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI81656"},{"key":"e_1_2_8_46_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.00490.2015"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjcmm.13913","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.13913","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,7,12]],"date-time":"2024-07-12T03:29:40Z","timestamp":1720754980000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.13913"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,11,5]]},"references-count":45,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,1]]}},"alternative-id":["10.1111\/jcmm.13913"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.13913","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"value":"1582-1838","type":"print"},{"value":"1582-4934","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,11,5]]},"assertion":[{"value":"2018-04-25","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-08-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-11-05","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}