{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,9,17]],"date-time":"2025-09-17T16:04:00Z","timestamp":1758125040715,"version":"3.37.3"},"reference-count":43,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,11,1]],"date-time":"2018-11-01T00:00:00Z","timestamp":1541030400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Basic Research Program of China","doi-asserted-by":"crossref","award":["2017YFA0504201"],"award-info":[{"award-number":["2017YFA0504201"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001809","name":"National Nature Science Foundation of China","doi-asserted-by":"crossref","award":["81720108017"],"award-info":[{"award-number":["81720108017"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Shanghai Jiao Tong University Medicine-Engineering Fund","award":["YG2017ZD11"],"award-info":[{"award-number":["YG2017ZD11"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>We previously identified the mouse dynein axonemal intermediate chain 2 (<jats:italic>Dnaic2<\/jats:italic>) gene. This gene expresses a component of the axonemal dynein complex that functions in cilia or flagella. We found that overexpression of <jats:italic>Dnaic2<\/jats:italic> results in female subfertility and male infertility. In this study, we generated <jats:italic>Dnaic2<\/jats:italic> knockdown (<jats:styled-content style=\"fixed-case\">KD<\/jats:styled-content>) mice and identified the potential regulatory mechanisms involved in <jats:italic>Dnaic2<\/jats:italic> function. For phenotype analysis, we found that body weight was lighter and size was smaller in <jats:italic>Dnaic2 <\/jats:italic><jats:styled-content style=\"fixed-case\">KD<\/jats:styled-content> mice than in wild\u2010type mice. A total of 45% of these <jats:italic>Dnaic2 <\/jats:italic><jats:styled-content style=\"fixed-case\">KD<\/jats:styled-content> mice were infertile due to sperm abnormalities in males, or had oocyte abnormalities and pathological changes in the tunica mucosa in the oviduct of females. Moreover, <jats:italic>Dnaic2<\/jats:italic> overexpression enhanced the expression of proliferating cell nuclear antigen (<jats:styled-content style=\"fixed-case\">PCNA<\/jats:styled-content>) in the ovaries, which suggested that <jats:italic>Dnaic2<\/jats:italic> stimulated proliferation of cells in the ovaries. However, <jats:styled-content style=\"fixed-case\">PCNA<\/jats:styled-content> expression in the testis of <jats:italic>Dnaic2<\/jats:italic>\u2010overexpressed mice was lower than that in controls. Additionally, the ratio of <jats:italic>Bax<\/jats:italic>\/B\u2010cell lymphoma\u20102<jats:italic>(Bcl\u20102)<\/jats:italic> in the testis of <jats:italic>Dnaic2<\/jats:italic>\u2010overexpressed mice was higher than that in controls, which suggested that <jats:italic>Dnaic2<\/jats:italic> inhibited cellular proliferation in the testis. To examine the molecular action of <jats:italic>Dnaic2<\/jats:italic>, immunoprecipitation analysis was used and showed that Dnaic2 protein interacted with signal transducer and activator of transcription 3 (Stat3). Molecular modelling analysis showed that Dnaic2 bound with the linker and <jats:styled-content style=\"fixed-case\">SH<\/jats:styled-content>2 domains of Stat3. Furthermore, overexpression of <jats:italic>Dnaic2<\/jats:italic> promoted phosphorylation of Stat3. In conclusion, our study suggests that <jats:italic>Dnaic2<\/jats:italic> plays a role in oogenesis and spermatogenesis by activation of Stat3.<\/jats:p>","DOI":"10.1111\/jcmm.13945","type":"journal-article","created":{"date-parts":[[2018,11,2]],"date-time":"2018-11-02T04:10:57Z","timestamp":1541131857000},"page":"417-425","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Dynein axonemal intermediate chain 2 plays a role in gametogenesis by activation of Stat3"],"prefix":"10.1111","volume":"23","author":[{"given":"Zhaojuan","family":"Yang","sequence":"first","affiliation":[{"name":"Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education) Bio\u2010X Institutes Shanghai Jiao Tong University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bo","family":"Xu","sequence":"additional","affiliation":[{"name":"Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education) Bio\u2010X Institutes Shanghai Jiao Tong University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaopeng","family":"Hu","sequence":"additional","affiliation":[{"name":"Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education) Bio\u2010X Institutes Shanghai Jiao Tong University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoying","family":"Yao","sequence":"additional","affiliation":[{"name":"Obstetrics and Gynecology Hospital Fudan University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunhui","family":"Tang","sequence":"additional","affiliation":[{"name":"Obstetrics and Gynecology Hospital Fudan University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cuifeng","family":"Qian","sequence":"additional","affiliation":[{"name":"Obstetrics and Gynecology Hospital Fudan University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shuzeng","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education) Bio\u2010X Institutes Shanghai Jiao Tong University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haifeng","family":"Chen","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Biotechnology Shanghai Jiao Tong University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaohui","family":"Bai","sequence":"additional","affiliation":[{"name":"School of Life Sciences and Biotechnology Shanghai Jiao Tong University Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6424-9783","authenticated-orcid":false,"given":"Ji","family":"Wu","sequence":"additional","affiliation":[{"name":"Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders (Ministry of Education) Bio\u2010X Institutes Shanghai Jiao Tong University Shanghai China"},{"name":"Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education Ningxia Medical University Yinchuan China"},{"name":"Shanghai Key Laboratory of Reproductive Medicine Shanghai China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2018,11]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.2741\/2339"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.052658699"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1186\/1477-7827-1-107"},{"volume-title":"The Physiology of Reproduction","year":"1994","author":"Sharpe RM","key":"e_1_2_7_5_1"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0015-0282(98)00401-4"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1242\/dev.122.6.1703"},{"key":"e_1_2_7_8_1","doi-asserted-by":"crossref","first-page":"841","DOI":"10.1210\/mend.13.6.0306","article-title":"Targeted expression of Bcl\u20102 in mouse oocytes inhibits ovarian follicle atresia and prevents spontaneous and chemotherapy\u2010induced oocyte apoptosis in\u00a0vitro","volume":"13","author":"Morita Y","year":"1999","journal-title":"Mol Endocrinol"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1038\/5985"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod63.6.1683"},{"key":"e_1_2_7_11_1","first-page":"3","article-title":"Control of early ovarian follicular development","volume":"54","author":"McNatty KP","year":"1999","journal-title":"J Reprod Fertil Suppl"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1038\/330181a0"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cb.10.110194.002011"},{"key":"e_1_2_7_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(02)01303-9"},{"key":"e_1_2_7_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(02)01302-7"},{"key":"e_1_2_7_16_1","doi-asserted-by":"crossref","first-page":"2409","DOI":"10.1242\/dev.124.12.2409","article-title":"The microtubule motor cytoplasmic dynein is required for spindle orientation during germline cell divisions and oocyte differentiation in Drosophila","volume":"124","author":"McGrail M","year":"1997","journal-title":"Development"},{"key":"e_1_2_7_17_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7696(08)62098-7"},{"issue":"3","key":"e_1_2_7_18_1","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1242\/jcs.107.3.497","article-title":"Molecular analysis of the gamma heavy chain of Chlamydomonas flagellar outer\u2010arm dynein","volume":"107","author":"Wilkerson CG","year":"1994","journal-title":"J Cell Sci"},{"key":"e_1_2_7_19_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.physiol.69.040705.141253"},{"key":"e_1_2_7_20_1","doi-asserted-by":"publisher","DOI":"10.1095\/biolreprod.105.041152"},{"key":"e_1_2_7_21_1","first-page":"457","article-title":"Primary ciliary dyskinesia (the immotile cilia syndrome)","volume":"73","author":"Schidlow DV","year":"1994","journal-title":"Ann Allergy"},{"key":"e_1_2_7_22_1","doi-asserted-by":"publisher","DOI":"10.1089\/dna.2008.0752"},{"key":"e_1_2_7_23_1","doi-asserted-by":"publisher","DOI":"10.1093\/jmcb\/mjq043"},{"key":"e_1_2_7_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajhg.2008.10.001"},{"key":"e_1_2_7_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2008.04.049"},{"key":"e_1_2_7_26_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gku340"},{"key":"e_1_2_7_27_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bti770"},{"key":"e_1_2_7_28_1","doi-asserted-by":"publisher","DOI":"10.1002\/elps.200900140"},{"key":"e_1_2_7_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu097"},{"key":"e_1_2_7_30_1","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.113.4.835"},{"key":"e_1_2_7_31_1","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.9.9.2337"},{"key":"e_1_2_7_32_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI0215650"},{"key":"e_1_2_7_33_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI119869"},{"key":"e_1_2_7_34_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI3785"},{"key":"e_1_2_7_35_1","doi-asserted-by":"publisher","DOI":"10.1097\/TP.0b013e3181739d25"},{"key":"e_1_2_7_36_1","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.18.5.2553"},{"key":"e_1_2_7_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.lungcan.2008.05.025"},{"key":"e_1_2_7_38_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.15.4261"},{"key":"e_1_2_7_39_1","doi-asserted-by":"publisher","DOI":"10.1101\/gad.12.13.2048"},{"key":"e_1_2_7_40_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.6.2846"},{"key":"e_1_2_7_41_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80011-4"},{"key":"e_1_2_7_42_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.1.527"},{"key":"e_1_2_7_43_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M407094200"},{"key":"e_1_2_7_44_1","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.1999.0365"}],"container-title":["Journal of Cellular and Molecular Medicine"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fjcmm.13945","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/jcmm.13945","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,15]],"date-time":"2023-09-15T20:26:29Z","timestamp":1694809589000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/jcmm.13945"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,11]]},"references-count":43,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2019,1]]}},"alternative-id":["10.1111\/jcmm.13945"],"URL":"https:\/\/doi.org\/10.1111\/jcmm.13945","archive":["Portico"],"relation":{},"ISSN":["1582-1838","1582-4934"],"issn-type":[{"type":"print","value":"1582-1838"},{"type":"electronic","value":"1582-4934"}],"subject":[],"published":{"date-parts":[[2018,11]]},"assertion":[{"value":"2018-07-09","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-09-10","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2018-11-01","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}