{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T04:24:43Z","timestamp":1789705483502,"version":"4.0.1"},"reference-count":34,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,11,5]],"date-time":"2018-11-05T00:00:00Z","timestamp":1541376000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["8150021"],"award-info":[{"award-number":["8150021"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81102706"],"award-info":[{"award-number":["81102706"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Picroside <jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> (P\u2010<jats:styled-content style=\"fixed-case\">II<\/jats:styled-content>), one of the main active components of scrophularia extract, which have anti\u2010oxidative, anti\u2010inflammatory effects, but its effect on hyperhomocysteinemia (<jats:styled-content style=\"fixed-case\">HH<\/jats:styled-content>cy) induced endothelial injury remains to be determined. Here, we test whether P\u2010<jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> protects <jats:styled-content style=\"fixed-case\">HH<\/jats:styled-content>cy\u2010induced endothelial dysfunction against oxidative stress, inflammation and cell apoptosis. In vitro study using <jats:styled-content style=\"fixed-case\">HUVEC<\/jats:styled-content>s, and in hyperhomocysteinemia mouse models, we found that <jats:styled-content style=\"fixed-case\">HH<\/jats:styled-content>cy decreased endothelial <jats:styled-content style=\"fixed-case\">SIRT<\/jats:styled-content>1 expression and increased <jats:styled-content style=\"fixed-case\">LOX<\/jats:styled-content>\u20101 expression, subsequently causing reactive oxygen species generation, up\u2010regulation of <jats:styled-content style=\"fixed-case\">NADPH<\/jats:styled-content> oxidase activity and <jats:styled-content style=\"fixed-case\">NF<\/jats:styled-content>\u2010\u03baB activation, thereby promoting pro\u2010inflammatory response and cell apoptosis. Blockade of Sirt1 with Ex527 or si<jats:styled-content style=\"fixed-case\">RNASIRT<\/jats:styled-content>1 increased <jats:styled-content style=\"fixed-case\">LOX<\/jats:styled-content>\u20101 expression, whereas overexpression of <jats:styled-content style=\"fixed-case\">SIRT<\/jats:styled-content>1 decreased <jats:styled-content style=\"fixed-case\">LOX<\/jats:styled-content>\u20101 expression markedly. P\u2010<jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> treatment significantly increased <jats:styled-content style=\"fixed-case\">SIRT<\/jats:styled-content>1 expression and reduced <jats:styled-content style=\"fixed-case\">LOX<\/jats:styled-content>\u20101 expression, and protected against endothelial cells from Hcy\u2010induced oxidative injury, inflammation and apoptosis. However, blockade of <jats:styled-content style=\"fixed-case\">SIRT<\/jats:styled-content>1 or overexpression of <jats:styled-content style=\"fixed-case\">LOX<\/jats:styled-content>\u20101 attenuated the therapeutic effects of P\u2010<jats:styled-content style=\"fixed-case\">II<\/jats:styled-content>. In conclusion, our results suggest that P\u2010<jats:styled-content style=\"fixed-case\">II<\/jats:styled-content> prevents the Hcy induced endothelial damage probably through regulating the <jats:styled-content style=\"fixed-case\">SIRT<\/jats:styled-content>1\/<jats:styled-content style=\"fixed-case\">LOX<\/jats:styled-content>\u20101 signaling pathway.<\/jats:p>","DOI":"10.1111\/jcmm.13949","type":"journal-article","created":{"date-parts":[[2018,11,5]],"date-time":"2018-11-05T12:03:47Z","timestamp":1541419427000},"page":"464-475","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Picroside <scp>II<\/scp> attenuates hyperhomocysteinemia\u2010induced endothelial injury by reducing inflammation, oxidative stress and cell 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