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Although obtaining normal and pathological reticulocyte transcriptome profiles should contribute greatly to our understanding of the molecular bases of terminal erythroid differentiation as well as the mechanisms of the hematological diseases, a basic limitation of these studies is the difficulty of efficient reticulocyte <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> isolation from human peripheral blood. The restricted number of possible parallel experiments primarily concern healthy individuals with the lowest number of reticulocytes in the peripheral blood and a low <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> content. In the present study, an efficient method for reticulocyte <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> isolation from healthy individuals and hemolytic anaemia patients is presented. The procedure includes leukofiltration, Ficoll\u2010Paque gradient centrifugation, Percoll gradient centrifugation, and negative (<jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>45 and <jats:styled-content style=\"fixed-case\">CD<\/jats:styled-content>61) immunomagnetic separation. This relatively fast and simple four\u2010stage method was successfully applied to obtain a reticulocyte\u2010rich population from healthy subjects, which was used to efficiently isolate the high\u2010quality <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> essential for successful <jats:styled-content style=\"fixed-case\">NGS<\/jats:styled-content>\u2010based transcriptome analysis.<\/jats:p>","DOI":"10.1111\/jcmm.13951","type":"journal-article","created":{"date-parts":[[2018,11,19]],"date-time":"2018-11-19T06:44:12Z","timestamp":1542609852000},"page":"487-496","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":8,"title":["Efficient method for isolation of reticulocyte RNA from healthy individuals and hemolytic anaemia patients"],"prefix":"10.1111","volume":"23","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9609-2979","authenticated-orcid":false,"given":"Micha\u0142","family":"Skulski","sequence":"first","affiliation":[{"name":"Department of Cytobiochemistry Faculty of Biotechnology University of Wroc\u0142aw Wroc\u0142aw Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2864-6757","authenticated-orcid":false,"given":"Rafa\u0142","family":"Bartoszewski","sequence":"additional","affiliation":[{"name":"Department of Biology and Pharmaceutical Botany Faculty of Pharmacy with Subfaculty of Laboratory Medicine Medical University of Gda\u0144sk Gda\u0144sk Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9118-0005","authenticated-orcid":false,"given":"Micha\u0142","family":"Majkowski","sequence":"additional","affiliation":[{"name":"Department of Cytobiochemistry Faculty of Biotechnology 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