{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,26]],"date-time":"2026-10-26T10:55:41Z","timestamp":1793012141739,"version":"4.5.0"},"reference-count":21,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2018,12,7]],"date-time":"2018-12-07T00:00:00Z","timestamp":1544140800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004622","name":"KWF Kankerbestrijding","doi-asserted-by":"publisher","award":["13\u20106001"],"award-info":[{"award-number":["13\u20106001"]}],"id":[{"id":"10.13039\/501100004622","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005357","name":"Agent\u00fara na Podporu V\u00fdskumu a V\u00fdvoja","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100005357","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["J Cellular Molecular Medi"],"published-print":{"date-parts":[[2019,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Germ cell tumours predominantly of the testis ((T)GCTs) are remarkably chemotherapy sensitive. However, a small proportion of patients fail to be cured with cisplatin\u2010based combination chemotherapy. miR\u2010371a\u20103p is a new liquid biopsy biomarker for (T)GCTs. The aim of this study was to evaluate clinical utility of plasma miR\u2010371a\u20103p level in patients starting systemic chemotherapy. Patients were included before the first cycle (N\u00a0=\u00a0180) and second cycle (N\u00a0=\u00a0101) of systemic first line chemotherapy, treated between July 2010 and May 2017. Plasma miR\u2010371a\u20103p levels were measured with the ampTSmiR test and compared to disease characteristics and outcome. Pretreatment plasma miR\u2010371a\u20103p levels were increased in 51.7% of cases and associated with number of metastatic sites, presence of lung, retroperitoneal, and mediastinal lymph node metastases, S \u2013 stage, IGCCCG risk group, and response to therapy. Patients with a negative pretreatment plasma level had better progression\u2010free survival (<jats:styled-content style=\"fixed-case\">PFS<\/jats:styled-content>) and overall survival (<jats:styled-content style=\"fixed-case\">OS<\/jats:styled-content>) compared to patients being positive for miR\u2010371a\u20103p (hazard ratio [HR]\u00a0=\u00a00.26, 95% confidence interval [CI] 0.09\u20100.71, <jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.02 for PFS and HR\u00a0=\u00a00.21, 95% CI 0.07\u20100.67, <jats:italic>P\u00a0<\/jats:italic>=<jats:italic>\u00a0<\/jats:italic>0.03 for OS, respectively). Patients negative for miR\u2010371a\u20103p in both samples had a superior PFS (HR\u00a0=\u00a00.10, 95% CI 0.01\u201021.49, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.02) and OS (HR\u00a0=\u00a00.08, 95% CI 0.01\u201027.81, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.008) compared to patients with miR\u2010371a\u20103p positive in both samples (multivariate analyses were non\u2010significant). In total 68% of the patients were S0. This study demonstrates clinical value of plasma miR\u2010371a\u20103p level in chemotherapy na\u00efve (T)GCT patients starting first line of chemotherapy to predict prognosis.<\/jats:p>","DOI":"10.1111\/jcmm.14013","type":"journal-article","created":{"date-parts":[[2018,12,8]],"date-time":"2018-12-08T05:10:38Z","timestamp":1544245838000},"page":"1128-1136","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":61,"title":["Clinical utility of plasma miR\u2010371a\u20103p in germ cell tumors"],"prefix":"10.1111","volume":"23","author":[{"given":"Michal","family":"Mego","sequence":"first","affiliation":[{"name":"Translational Research Unit Faculty of Medicine Comenius University and National Cancer Institute Bratislava Slovakia"},{"name":"2nd Department of Oncology Faculty of Medicine Comenius University and National Cancer Institute Bratislava Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8795-109X","authenticated-orcid":false,"given":"Ton","family":"van Agthoven","sequence":"additional","affiliation":[{"name":"Department of Pathology Laboratory for Experimental Patho\u2010Oncology Erasmus MC University Medical Center Cancer Institute Rotterdam The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paulina","family":"Gronesova","sequence":"additional","affiliation":[{"name":"Cancer Research Institute Biomedical Research Center Slovak Academy of Sciences Bratislava Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michal","family":"Chovanec","sequence":"additional","affiliation":[{"name":"2nd Department of Oncology Faculty of Medicine Comenius University and National Cancer Institute Bratislava Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vera","family":"Miskovska","sequence":"additional","affiliation":[{"name":"1st Department of Oncology Faculty of Medicine Comenius University and St. Elisabeth Cancer Institute Bratislava Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jozef","family":"Mardiak","sequence":"additional","affiliation":[{"name":"2nd Department of Oncology Faculty of Medicine Comenius University and National Cancer Institute Bratislava Slovakia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8146-1911","authenticated-orcid":false,"given":"Leendert H. 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